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26-09-2014 13:48

Rubén Martínez-Gil Rubén Martínez-Gil

Hola a todos. Subo unas fotos de una Pulvinula qu

26-09-2014 15:13

Alessio Pierotti Alessio Pierotti

can someone help me with this work ?Booth, C.; O'R

25-09-2014 08:22

Blasco Rafael Blasco Rafael

Hola, Tengo ESTAS Muestras Recogidas en Hayedo - a

24-09-2014 20:25

Enrique Rubio Enrique Rubio

Bonsoir les amis Peut etre j'avais recolté le me

24-09-2014 19:43

Bernard Declercq Bernard Declercq

Good evening,does anyone have following paper for

22-09-2014 22:08

Rubén Martínez-Gil Rubén Martínez-Gil

Hola a todos. Subo fotos de una Peziza que he enc

22-09-2014 08:03

Beñat Jeannerot Beñat Jeannerot

Adio à tous, Cette Helvella, récoltée aux Oule

23-09-2014 18:12

Blasco Rafael Blasco Rafael

Hola, esta muestra creo que al final sea Bisporell

23-09-2014 17:34

Blasco Rafael Blasco Rafael

Hola, tengo esta Peziza recogida en Hayedo--Abetal

24-09-2014 05:40

Roland Labbé

Bonjour !Est-ce quelqu'un parmi vous aurait une de

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Help in the identification.
Lepista Zacarias, 18-09-2014 22:32
I thought that this one was a lichen, but it lacks an alga. It was growing on a shrub with smooth bark, in a calcareous habitat, in the margins of a small streamlet without water in summer.

Asci with 8 spores each; The spores are 1-septate when young and then develop other septa, up to 8-septate.
Asci: up to 215 x 12 um;
Spores:
(26.7) 29.2 - 33.7 (34.5) x (6.2) 6.5 - 7.9 (8.1) µm
Q = (3.9) 4 - 4.76 (4.8) ; N = 20
Me = 31.2 x 7.2 µm ; Qe = 4.4,


I will appreciate any hint for the classification.
Thanks in advance,
zaca

  • message #31333
  • message #31333
  • message #31333
  • message #31333
Hans-Otto Baral, 18-09-2014 22:39
Hans-Otto Baral
Re : Help in the identification.
Did you test the iodine reaction? I think it is a lichen with a whitish thallus.
Lepista Zacarias, 18-09-2014 22:44
Re : Help in the identification.
Thanks, Zotto, for your message.
In fact, I thought this was a lichen, but I found no trace of an alga.
The last two sets of photos (except for the  one on the left hand of the last) were taken after join a drop of melzer to the slide. This permits to see clearly the septation of the spores, which was not possible without it.
Thanks again,
zaca
Hans-Otto Baral, 18-09-2014 22:53
Hans-Otto Baral
Re : Help in the identification.
o.k., this would be interesting to look more closely. With oil immersion I am sure you will see the septa, but different. It seems the cells are ocellate, and the septum gets thicker when killed by MLZ.

It is well possible that the asci are inamyloid. Anyhow I would try Lugol for a possible hemiamyloid reaction.

The spores ar very characteristic, I think a lichenologist knows them.
Lepista Zacarias, 18-09-2014 23:08
Re : Help in the identification.
Dear, Zotto,
I will follow your suggestions and try a new preparation, whenever possible. If I'll find anything different, it will be reported here.
Thanks,
zaca
Alain GARDIENNET, 19-09-2014 07:24
Alain GARDIENNET
Re : Help in the identification.

Hi Zaca,


Like Zotto, I think it could be a lichen, with leprose thallus.


You can test chemical reactions on the thallus : K, P, C, KC.


How are ascomatas ? Perithecias ? Apothecias ?


Alain

Lepista Zacarias, 19-09-2014 20:40
Re : Help in the identification.
Problem with solution in sight: Strigula stigmatella.

The ascomata are perithecia. I made the spot tests and all chemical reactions were negative. Moreover, did new microscopic observation and found that there seems to be a brown or golden-green alga (perhaps of the genus Trentepohlia). I checked the reference mentioned below and, taking into account the microscopic data, found two genera of pyrenocarpous to which my specimen can belong: Porina or Strigula. I found, in particular, a species _Strigula stigmatella_ with similar features.
Many Thanks to Zotto and to Alain, for their comments that lead me to the possible solution,
zaca


Ref:
Alan Orange, British and Other Pyrenocarpous Lichens, 2013.
(available at: https://www.museumwales.ac.uk/media/13849/Orange,-A.-(2013)-British-and-other-pyrenocarpous-lichens.pdf)