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04-02-2014 21:22
Christian LechatDear friends,I would need the following document:
bonjour à tous,
I wonder if someone can help me with this Ascomycete I have collected from the South of England on Fagus wood....
Collected 14/2/2013 and 2/2/2014
Lat. 51.0949, Long. -0.1832 at 105m elevation
Apothecia up to 1.5mm, whitish, without stalk
Ascospores 18.1-23.3 x 3.7-4.3. Becoming (at least?) 1-septate inside the asci, finally 3-septate
Croziers present. Ascus tips amyloid - Calycina type
Paraphyses more or less vacuolar (vacuoles not strongly refractive)
Excipulum of hyaline cells
Marginal hairs not observed
I think it might fit somewhere in the genus Calycellina, but I'm far from 100% sure....
Merci pour votre aide,
Nick
does the photo on upper right show paraphyses or the margin? Could you try a section showing the excipulum at flanks and margin?
Also helpful would be if you have living asci. The figured one is dead, and it is valuable to know whether the spores are septate already inside the living asci.
Are the vacuoles in the paraphyses of low or high contrast? I assume of low, judging from the elements on your photo.
Prersently I have no idea at all.
Zotto
Hi Zotto (and eveyone),
Aha! After much searching & sectioning I found some hairs at the margin!
I think the ascus pictured is alive (?) - The septae are difficult to see because of the guttules but I'm convinced the spores are 1-septate (rarely 3-septate) before release.
The photo is of paraphyses. Yes, I think low contrast vacuoles.
Several of the apothecia have asci with 8 globose spores - Can this be a Helicogonium?
Thanks again for your help,
Nick
Sorry, I overlooked that you wrote low-refractive for the VBs. I would exclude Calycellina. maybe the fungus could be sought in Pseudohelotium, but this was a waste basket genus in earlier times, not sure how narrow it should be taken today.
In the few Pseudohelotium species for which I have images there occur similar, partly also roughened hairs. So I place your fungus in that folder.
The globose spores are either immature or abnormal, difficult to say.
Is your drawing from 2013 and all photos from 2014?
Zotto
Hi Zotto,
Yes, it's quite possible that spores are 3-septate on discharge.
I must say I interpreted the roughened hairs as smooth ones with small droplets attached - I will try to clarify this. Is there a solution I can use to clear the droplets (if they are indeed there?)
The three images in my original post (macro photo, drawing, collage of photos) are from 2013. Subsequent photos are 2014.
Thanks,
Nick
I would test Melzer or Lactophenol, and also KOH. If it resists in one of these, it should not be resinous, But the classification of these deposits is not really clear.
Zotto
this species give me a strong resemblence to the Arachnopezizoide group although no subiculum is visible, I have no idea about the species but I found the name Arachnopeziza nuda Korf on the internet, this species is described in: Bull. natn. Sci. Mus., Tokyo 4: 393 (1959)?. This is just a wild shot in the dark because I don't have the description. If anyone has this article I am interested in it.
There is one record of it in the Bristol aerea on GBIF: http://www.gbif.org/occurrence/search?taxon_key=3488500&GEOREFERENCED=true&SPATIAL_ISSUES=false?
Regards,
Stip
Hi Zotto & Stip,
Thanks for your input
Great - this description of A.nuda actually fits very well!
The hyphae at the base of the apothecia are quite abundant, and I think I would say they are "fairly thick walled" (at least thicker than the walls of the excipulum cells):
I'll dry my collection to see if it becomes yellow...
Many thanks to the three of you !
Amitiés
Michel
Stip