Hyaloscypha sp. ? - Forum ASCOFrance

11-02-2019 23:39

Hyaloscypha sp. ?

Edouard Evangelisti

Dear all, Would you agree this is likely a Hyalos

11-02-2019 21:13

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Hyaloscypha sp. ?

Edouard Evangelisti, 11-02-2019 23:39

Dear all,

Would you agree this is likely a Hyaloscypha?

Unfortunately I cannot provide good enough data to solve the species since these were found by chance on a wood sample (I still need to check whether it was coniferous) harvested for other purposes (a Mucronella species).

Briefly, apothecia were tiny (< 1 mm), whitish-translucent, with dense hairs on the excipulum. I could check that the asci were IKI+ and developed from croziers. Paraphyses are cylindrical. Hairs were smooth, hyaline, cylindrical, slightly tapering at the top, not reacting to iodine. I could not get mature spores, but a few of them within asci were 8-9 x 1.5-2 µm, hyaline, smooth, fusiforms and often arcuate.

Many thanks for your help.

With my best wishes,

Edouard

Kosonen Timo, 12-02-2019 06:19

Re : Hyaloscypha sp. ?

Hello,

IF substratum happens to be coniferous, H. aureliella is a good alternative. A slide with excipular cells in MLZ reagent would be helpfull (amyloid nodules?). There's no resin visible in the photos provided, but there are always exceptions. Pick the most mature fruitbody and make a careful mount with water.

If hardwood and absolutely no MLZ reactions (hairs, excipula), it could be a Hyaloscypha look-a-like, just "outside" Hyaloscypha :-).

cheers,

Timo

Hans-Otto Baral, 12-02-2019 07:55

Re : Hyaloscypha sp. ?

Did you measure the hairs? Do they have the same scale as the asci? If yes, then the hairs are pretty small. Which region of ?France does it come from?
Was the substate exposed, a still-attached branch? Or on the moist gorund?
The MLZ reaction of hairs and exciple is used in Huhtinen's key as a character but one must know that it is not at all shown in Lugol.

Edouard Evangelisti, 12-02-2019 21:40

Re : Hyaloscypha sp. ?

Dear Timo and Zotto,

Thanks a lot for your messages!

I should have mentioned that, actually these are collections from the Suffolk (Brandon, UK). I am in Cambridge for work at the moment, that is why I do not have access to my usual microscope and reagents.

I will try my best to save some samples for further observations on rehydrated samples (still better than nothing I guess) when I am back to France.

I can confirm this was coniferous wood (picture enclosed). It was on the ground, and quite rotten already. Thanks for the tip regarding the use of Melzer vs Lugol for reaction with hairs, I was not aware of that.

I will go back there and see if I can collect older specimens to do more observations, including a more careful look at the resin and to measure hairs.

Cheers,
Edouard

Hans-Otto Baral, 12-02-2019 22:13

Re : Hyaloscypha sp. ?

Yes, it is coniferous, so Timo's suggestion (aureliella) is not ruled out.

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