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Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Bernard CLESSE,
22-07-2016 22:56
Voici un petit asco jaune stipité, trouvé dans une ornière forestière humide sur feuilles pourries de peuplier noir (Populus nigra) :a) spores fusiformes, remplies de microguttules, triseptées (parois très fines, pas toujours faciles à voir et déjà présentes dans l'asque) : 16,5-26x5-7
b) paraphyses cylindriques multiguttulées
c) asques IKI+
Bernard
Hans-Otto Baral,
22-07-2016 23:03
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Reminds somewhat a Phaeohelotium, but were the spores septate inside the living asci?
Bernard CLESSE,
23-07-2016 07:24
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Hi Zotto,
Glad to read you.
Yes, spores seem regularly septate in the ascus, as in this picture.
Bernard
Glad to read you.
Yes, spores seem regularly septate in the ascus, as in this picture.
Bernard
Hans-Otto Baral,
23-07-2016 07:44
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
I am sorry, that was not my question :-(
I saw this well but important is: are they septate in the living ascus.
Septa are common in hymenoscyphus and Phaeohelotium, but only in overmature spores. Did you see turgescent asci?
I saw this well but important is: are they septate in the living ascus.
Septa are common in hymenoscyphus and Phaeohelotium, but only in overmature spores. Did you see turgescent asci?
Bernard CLESSE,
23-07-2016 09:25
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Sorry Zotto, I did not understand your request… :o)
I found some living asci and saw spores with septa.
Bernard
I found some living asci and saw spores with septa.
Bernard
Bernard Declercq,
23-07-2016 09:45
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Hi Bernard and Zotto,
This species has been collected several times in Belgium, Flanders , a.o. on leaves of Populus. I tentatively called it Hymenoscyphus cf. strangulatus (Velen.) Svr?ek, the only Western European Hymenoscyphus species I know producing 3-septate spores within living asci, but it needs a new name.
Bernard
This species has been collected several times in Belgium, Flanders , a.o. on leaves of Populus. I tentatively called it Hymenoscyphus cf. strangulatus (Velen.) Svr?ek, the only Western European Hymenoscyphus species I know producing 3-septate spores within living asci, but it needs a new name.
Bernard
Bernard CLESSE,
23-07-2016 09:48
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Bonjour Bernard,
Très content de te lire. Merci pour ton avis sur cet asco !
Amitiés,
Bernard
Très content de te lire. Merci pour ton avis sur cet asco !
Amitiés,
Bernard
Hans-Otto Baral,
23-07-2016 10:50
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Ah, thanks Bernard D., I remember your drawing under the name H. triseptatus.
And thanks Bernard C., the photos are splendid!
What I still wonder:
- B.D. draws an apothecium with a stipe longer than apo diameter, how long are the stipes in your sample?
- I think your asci have croziers, do you have a clearer photo or did you see that?
It seems I never met this fungus, which could belong in the relationship of H. menthae which stands quite apart between Hymenoscyphus and Phaeohelotium.
Zotto
And thanks Bernard C., the photos are splendid!
What I still wonder:
- B.D. draws an apothecium with a stipe longer than apo diameter, how long are the stipes in your sample?
- I think your asci have croziers, do you have a clearer photo or did you see that?
It seems I never met this fungus, which could belong in the relationship of H. menthae which stands quite apart between Hymenoscyphus and Phaeohelotium.
Zotto
Bernard CLESSE,
23-07-2016 10:56
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
OK Zotto, I check the length of the stipes and the presence of croziers at the base of asci.
Bernard
Bernard
Bernard CLESSE,
23-07-2016 12:05
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
I took this third microscopic observation to redo more photos.
a) the mature spores are typically constricted at the septa
b) I think asci have a crozier at the base but it is not very clear to me
c) I also checked the length of the stems/diameter apothecia : 550/810 - 550/720 - 800/750
What do you think about that ?
Bernard
a) the mature spores are typically constricted at the septa
b) I think asci have a crozier at the base but it is not very clear to me
c) I also checked the length of the stems/diameter apothecia : 550/810 - 550/720 - 800/750
What do you think about that ?
Bernard
Hans-Otto Baral,
23-07-2016 15:31
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Yes, clearly with croziers. And the spores, do they produce these large microconidia?
Stalk length is fine!
Stalk length is fine!
Bernard CLESSE,
23-07-2016 17:17
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
I imagine Zotto but I can not confirm because I have more fresh material… :o(
Bernard
Bernard
Hans-Otto Baral,
23-07-2016 17:24
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
ah, it could be that anastomoses are formed between conidia (valuable for nuclear DNA exchange).
Bernard CLESSE,
23-07-2016 23:06
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Hi Bernard & Zotto,
I returned on site tonight and have found several copies of Hymenoscyphus cf. strangulatus. I'll let them ripen to see mature spores with any conidia.
I returned on site tonight and have found several copies of Hymenoscyphus cf. strangulatus. I'll let them ripen to see mature spores with any conidia.
But I redid the macro and micro pictures you can use.
Bernard
Hans-Otto Baral,
24-07-2016 07:27
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
thanks!
Bernard CLESSE,
26-07-2016 21:30
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Hi Zotto and Bernard,
I just make an observation on very mature apothecia.
I just make an observation on very mature apothecia.
Obviously mature spores, triseptate, break up at the walls and give conidia. These are sometimes interconnected by a filament (anastomoses between conidia was talking Zotto?)
What do you think about that ?
Bernard
Hans-Otto Baral,
26-07-2016 21:36
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
The spores disarticulate (break) at the septa, and they form anastomoses (bridges), but I don't see conidia, that was an error of mine.
Bernard CLESSE,
27-07-2016 00:06
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
OK Zotto, thank you for your message ! I had never seen this phenomenon before,
Bernard
Bernard
Luc Bailly,
27-07-2016 11:25
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
Bonjour à tous,
C'est peut-être parce que les spores sont trop mûres, et il y a une ou deux cellules qui sont mortes et se sont vidées de leur cytoplasme.
Amitiés - LUC.
C'est peut-être parce que les spores sont trop mûres, et il y a une ou deux cellules qui sont mortes et se sont vidées de leur cytoplasme.
Amitiés - LUC.
Bernard CLESSE,
27-07-2016 20:02
Re : Asco jaune sur feuilles pourries de peuplier noir (Populus nigra)
OK Luc, merci pour ton avis !
Amitiés,
Bernard
Amitiés,
Bernard