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26-01-2015 17:40

Miguel Ãngel Ribes Miguel Ángel Ribes

H againI have this green Propolis in Pinus pinea c

27-01-2015 00:13

Francisco Calaça Francisco Calaça

Hello to all!I'm looking for this paper: "Lundqvis

26-01-2015 13:21

Vasileios Kaounas Vasileios Kaounas

Found 26-01-15, in Greece, in Pistacia lentiscus i

24-01-2015 10:09

Nina Filippova

Dear forum,i am currenly working with collection o

25-01-2015 16:59

Blasco Rafael Blasco Rafael

Hola tengo esta muestra recogida en madera de Popu

25-01-2015 12:02

Leandro Sánchez Leandro Sánchez

Sur terrain sablonneux, dunes établies, près de

23-01-2015 08:26

brigitte vignot

bonjour trouvé sur écorce de feuillus (probable

22-01-2015 20:46

Miguel Ãngel Ribes Miguel Ángel Ribes

Hello, good nightI have this small (1-2 mm broad)

25-01-2015 09:45

Vasileios Kaounas Vasileios Kaounas

I want to ask a question. Can we only from a featu

25-01-2015 09:31

Nina Filippova

I was following Barr (1970) for identification of

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Propolis viridis 170115 416
Miguel Ángel Ribes, 26-01-2015 17:40
Miguel Ãngel RibesH again

I have this green Propolis in Pinus pinea cones and wood in a dune environment in Huelva. I think it fits Ok with P. viridis using Baral's key, but I prefer your opinion because I have a previos collection in Eucalyptus from Tenerife with a dubious identification.

Apos aprox 2-2,5x1 mm, green, in cones and woor of Pinus pinea.
Asci 8 spores, IKI -, without? croziers, (81.1) 84.8 - 104.9 (120.3) x (11.6) 12.3 - 15.9 (17.8) µm; Me = 95.8 x 14.3 µm.
Spores with elipsoidal shape, sometimes slightly alantoid, smooth, (15.9) 16.1 - 19.5 (21.5) x (6.6) 6.7 - 7.6 (8.1) µm; Q = (2.1) 2.2 - 2.7 (3.0); Me = 17.6 x 7.2 µm ; Qe = 2.5, with 2 big LBs, (2.3) 2.8 - 3.7 (3.9) x (2.2) 2.7 - 3.4 (3.8) µm; Q = 1.0 - 1.1 (1.2); Me = 3.3 x 3.1 µm ; Qe = 1.1.
Paraphysis thin, multibranched, not enlarged at top, agglutinated by a dark-green substance.
Excipulum (ectal and medullar) with textura intricata?
Margin green, higher than hymenium, with a palisade of perpendicular pirsmatical cells.

Thank you.

Miguel Á. Ribes
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Hans-Otto Baral, 26-01-2015 18:35
Hans-Otto Baral
Re : Propolis viridis 170115 416
Yes, undoubtedly! Interesting also your remark on croziers. I am not sure in your case, but I have no fro all of my taxa information about them. So far when I saw croziers they were always present in these propoloid genera.

Are your ascus data from dead asci? Because only the right of the free figured asci is alive.

Curious about your sample from Tenerife :-)

Zotto
Miguel Ángel Ribes, 26-01-2015 23:49
Miguel Ãngel Ribes
Re : Propolis viridis 170115 416
I don't know why the asci are dead, the collection is from 17/1/2015 and micro pictures are made in tap water on 20/1/2015, only 3 days latter. I have made new measurements of asci from hymenium cuts and this are, only a little bigger than previous: (87.7) 97.7 - 120.4 (123.5) x (11.7) 12.4 - 16.1 (17.1) µm; Me = 107.9 x 14.1 µm. Anycase, there is a big range of live asci measures, see picture, please.

Thank you.
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Hans-Otto Baral, 27-01-2015 10:59
Hans-Otto Baral
Re : Propolis viridis 170115 416
These are fine photos! They show strong variation in length which is normal, whereas the width is almost the same (ca. 5% difference). In your measurements the width varies between 12 and 17 µm and this is why I guess you included also dead asci. It is normal that dead asci are in a preparation of a fresh fungus.

From your scales I evaluate for the dead asci on your photo a width of 13-14 µ, while the living have 17-18 µm! Those on the present photo have 88-120 x 17.5-18 if I measure corrrectly. On your previous arrangement I measure +82-100 x 13-14 µm, *120 x 17 µm.
Miguel Ángel Ribes, 27-01-2015 14:12
Miguel Ãngel Ribes
Re : Propolis viridis 170115 416
I am going to hydrate some apo and try to find only alive asci this evening.

Thank you.
Miguel Ángel Ribes, 27-01-2015 20:05
Miguel Ãngel Ribes
Re : Propolis viridis 170115 416
Impossible to find another alive asci, nor from hand free cut nor taken a small portion with tweezers. Perhaps samples I found had more than 1 year in the field and they look like "fresh" because of the previous days rain. But I think spores are alive, isnt' it?

But at least the new revision has help me to check again for croziers with Congo Red, and I think it has croziers (picture above).

Thank you.

Miguel Á. Ribes
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Hans-Otto Baral, 27-01-2015 20:27
Hans-Otto Baral
Re : Propolis viridis 170115 416
Yes, surely croziers. And as I said, your beforelast photo shows wonderful living asci, and also the right one  in the compilation is turgescent.

Now I modified my ascus data with data from your collection to
asci *85–120 x 14–18 µm (†80–115 x 11–15)
Miguel Ángel Ribes, 27-01-2015 20:34
Miguel Ãngel Ribes
Re : Propolis viridis 170115 416
Ok, Zotto, many thanks.

Miguel Á. Ribes.
Hans-Otto Baral, 27-01-2015 20:39
Hans-Otto Baral
Re : Propolis viridis 170115 416
I am also thankful!