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18-02-2014 12:34

Peter Thompson

Hello Everyone,I have found a brownish operculate

18-02-2014 10:12

Garcia Susana

Hi all: I found these perithecia growing on the s

18-02-2014 00:31

Iglesias Plácido

Hola, tengo interés en el siguiente artículo: EC

17-02-2014 22:40

Rubén Martínez-Gil Rubén Martínez-Gil

Hola a todos.Pongo unas fotos de unas copitas que

17-02-2014 15:51

Chris Yeates Chris Yeates

Bonjour tousI have collected a Discosia on dead La

17-02-2014 16:18

Christian Lechat Christian Lechat

Hi to all,I would need the following document, doe

17-02-2014 16:57

Chris Yeates Chris Yeates

I have collected a Leptosphaeria on the dead stem-

15-02-2014 18:02

Björn Wergen Björn Wergen

Hi there,does anybody have this paper?MÜLLER, E.

16-02-2014 11:11

Alain GARDIENNET Alain GARDIENNET

Bonjour à tous, Auriez-vous cet article : New

15-02-2014 10:59

FRANCIS FOUCHIER

Bonjour je recherche une clé des Plectania-Donadi

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Possibly Ascobolus brassicae or Pseudombrophila bulbifera
Peter Thompson, 18-02-2014 12:34
Hello Everyone,

I have found a brownish operculate discomycete growing on rabbit dung.

I have had a look at previous postings on Ascofrance and think that either Ascobolus brassicae or Pseudombrophila bulbifera are the likely options. There is, however, a distinctive characteristic of my collection which I can not find in the literature - mature spores become brown in lugol.

An image of the fruit bodies is attached, along with a drawing of the microscopy. I have looked at two apothecia and the second had distinctly purple warts on the spores, whereas in the first sample the warts did not look purple. All other characteristics were the same in the two samples. Purple colouration is, of course, more likely in Ascobolus. 

Pseudombrophila dentata seems to be ruled out by spore diameter.

I would be grateful for confirmation of one of these, or alternative suggestions for my sample.

Thank you,
Peter Thompson.
  • message #27712
  • message #27712
Michel Delpont, 18-02-2014 15:12
Michel Delpont
Re : Possibly Ascobolus brassicae or Pseudombrophila bulbifera
Hello Peter.

In my opinion it is Ascobolus brassicae. Have you controlled the reaction of asci with Melzer, positive or negative?

Michel.
Peter Thompson, 18-02-2014 16:06
Re : Possibly Ascobolus brassicae or Pseudombrophila bulbifera
Hello Michel,

The Melzers reaction is negative on both the ascus tips and the spores. 

I am also seeing some paraphyses which are branched at their tips.

Both characteristics are consistent with Ascobolus brassicae.

With Best Wishes,
Peter.