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Wall or Sheath
Romina Sánchez,
03-05-2010 18:10
Regards
Romina
Christian Lechat,
03-05-2010 18:24
Re:Wall or Sheath
You would have to use Black ink to see the sheath.
Christian
Christian
Romina Sánchez,
03-05-2010 19:00
Re:Wall or Sheath
Thank you Chirstian, I appreciate your quick reply!
Romina
Romina
Hans-Otto Baral,
09-05-2010 00:13
Re:Wall or Sheath
I also recommend to use Cresyl blue (aqueous). Many gel sheaths stain bright lilac herein, but sometimes distinct sheaths are negative.
I also know the problem that the spore wall consists of several layers. F.ex. in Sarcoscypha the two layers are wall material, not gel sheath, but behave different: the outer layer is rigid and gets folded and separates when the inner elastic layer shrinks during turgor loss. The surface of the outer stains lilac in CRB which means a very thin gel on it.
Zotto
I also know the problem that the spore wall consists of several layers. F.ex. in Sarcoscypha the two layers are wall material, not gel sheath, but behave different: the outer layer is rigid and gets folded and separates when the inner elastic layer shrinks during turgor loss. The surface of the outer stains lilac in CRB which means a very thin gel on it.
Zotto
NC NC,
10-05-2010 17:56
Re:Wall or Sheath
thank you very much Zotto!
I have got ascospores with many layers and I whant to differentiate if the outter one is a sheat or not.
Your explanation is very useful, I will try with the cresyl blue.
Romina
I have got ascospores with many layers and I whant to differentiate if the outter one is a sheat or not.
Your explanation is very useful, I will try with the cresyl blue.
Romina
Romina Sánchez,
10-05-2010 18:00
Re:Wall or Sheath
Thank you very much Zotto!
I have got ascospores with many layers and I want to differentiate if the outer one is a sheath or not.
Your explanation is very useful, I will try with the Cresyl Blue.
Warm regards
Romina
I have got ascospores with many layers and I want to differentiate if the outer one is a sheath or not.
Your explanation is very useful, I will try with the Cresyl Blue.
Warm regards
Romina
Jacques Fournier,
10-05-2010 19:11
Re:Wall or Sheath
Hi Romina,
the sheaths of ascospores are of different chemical structure, which implies they react differently with stains. Aqueous nigrosin and chlorazol black also might be very useful, they sometimes also stain the appendages.
Cheers,
Jacques
the sheaths of ascospores are of different chemical structure, which implies they react differently with stains. Aqueous nigrosin and chlorazol black also might be very useful, they sometimes also stain the appendages.
Cheers,
Jacques
Romina Sánchez,
10-05-2010 22:18
Re:Wall or Sheath
Thanks Jacques!
the people in this forum is wonderful!
And and you three (Christian, Zotto and Jacques), I saw that always respond the doubts of everybody. It's great that you share your knowledge.
Thank you again.
Romi
the people in this forum is wonderful!
And and you three (Christian, Zotto and Jacques), I saw that always respond the doubts of everybody. It's great that you share your knowledge.
Thank you again.
Romi