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13-01-2021 19:14

Edvin Johannesen Edvin Johannesen

I though I'd just ask you about this tiny discomyc

22-12-2020 21:20

Robin Isaksson Robin Isaksson

Someone have a clue what this is? Spores 13-16x

13-01-2021 21:38

Dirk Baert

Dag Allen, Deze gevonden op schors van een afgeva

09-01-2021 15:58

Lothar Krieglsteiner Lothar Krieglsteiner

... collected 23.5.20, Northern Germany, Schorfhei

09-01-2021 16:21

Lothar Krieglsteiner Lothar Krieglsteiner

.. collected 14.2.20, Southern Germany, Baden-Wuer

09-01-2021 14:51

Lothar Krieglsteiner Lothar Krieglsteiner

... collected 9.6.20, Germany, Bavaria, Franconian

13-01-2021 10:54

Castillo Joseba Castillo Joseba

Me mandan elmaterial seco de Galicia,   parece q

12-01-2021 06:29

Khomenko Igor Khomenko Igor

Hi,I found this discomycete in two instances in Se

12-01-2021 22:32

Garcia Susana

Hola, He encontrado este anamorfo creciendo en gr

11-01-2021 22:37

Castillo Joseba Castillo Joseba

Me mandan el material en seco de Galicia,  recole

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Aff. Heterosphaeria on Populus tremula wood
Edvin Johannesen, 13-01-2021 19:14
Edvin JohannesenI though I'd just ask you about this tiny discomycete, which I struggle with. It is only about 0.2-0.4 mm in diameter. It was growing on a Populus tremula log, directly on the wood. The wood is hardly decomposed and very firm. It was collected last May, but I only recently received the dried specimen. It seems like the hymenium in many of the apothecia are now eaten by insects or have decomposed. Thus quite difficult to make nice, thin sections, so I apologise for that.

I am not even sure what family we're in. Initially, when I saw it in wet condition, I thought it was erumpent and was thinking Coccomyces or along those lines. Indeed some of the younger ascomata appear to be erumpent, but in the dried material I see that the dark-rimmed, somewhat dentate apothecia are sitting directly on the wood, anchored by a fringe of white hyphae (anchoring hyphae/subiculum). The hymenium is, when fresh whitish, when dry more greyish and somewhat pruinose.

The spores measure 12-15 x 5 microns, mostly 3-septate, hyaline, smooth. Some spores are smaller (7 x 4) and 1-septate. Many spores are germinating from one or both ends – I guess the specimen is over-mature. What I think are paraphyses are orange-tipped (irregularly flame-like). There are dark, transversely roughened hyphae in the excipulum (fringe) and some spherical cells in what may be subhymenium. The ascus tips are IKI+ (blue in Baral'sche Lösung with no KOH pre-treatment). Structures in the excipulum are stained greenish-blue with IKI, too, but I am not sure that it's due to amyloidity.

Here's a link to some photos. Photo names indicate the medium (KOH, IKI, PC (phase contrast)).

The best fit is in the genus Heterosphaeria, but I'm not sure if any species in that genus grows on wood.  Are we in Phacidiaceae?

Suggestions appreciated.  Thanks!  (I hope the link works)

Hans-Otto Baral, 13-01-2021 21:43
Hans-Otto Baral
Re : Aff. Heterosphaeria on Populus tremula wood
Too overmature to say much. Durella (Xylogramma) atrocyanea would be inamyloid. Any idea of the paraphyses?
Edvin Johannesen, 13-01-2021 22:13
Edvin Johannesen
Re : Aff. Heterosphaeria on Populus tremula wood
Difficult to find an intact hymenium. I thought maybe the three phase contrast images showed paraphyses with the golden structures apically. I will try again to see if I can find intact hymenium with paraphyses, but several attempts have failed so far.