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02-12-2017 08:36

Stephen Mifsud Stephen Mifsud

Hi, I got this mould as a contaminant while studyi

06-12-2017 22:54

Rubén Martínez Gil Rubén Martínez Gil

Hola a todos. Subo unas fotos de un Hymenoscyphus

06-12-2017 11:15

Elisabeth Stöckli

Bonjour, Trouvé au sol sur branche décortiquée

07-12-2017 13:24

Castillo Joseba Castillo Joseba

En hoja de Laurus ...A ver si hay alguna sugerenci

05-12-2017 13:20

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia,  en hoja de Lau

26-11-2017 11:37

Thorben Hülsewig

Hi there,i found this anamorph on a part of a plan

05-12-2017 11:34

Gilles Corriol Gilles Corriol

Bonjour à tous,Je n'arrive à rien pour cette pou

06-12-2017 20:14

Pintos Angel Pintos Angel

Hola, es esto una Keissleriella? Pseudotecio con p

05-12-2017 12:32

Savic Dragisa

Branch of Tamarix gallica lying on the ground (Ser

05-12-2017 20:47

Rubén Martínez Gil Rubén Martínez Gil

Hola a todos. Subo unas fotos de unos ascos que h

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Mould which easily break when mounted in water or Stains
Stephen Mifsud, 02-12-2017 08:36
Stephen MifsudHi, I got this mould as a contaminant while studying another microfungus inoculated from a decaying log. On various media (PDA, SDA, Czapek) it forms an olive green growth  (3cm / 14C / 7 days) with a yellowish tinge. First indication is that of a Penicillium sp. but under the microscope I could not make heads and tails. The prime character I can describe is that the phialides and hypha of the conidiogenesis apparatus breaksing up into  constituent pieces giving this polymorphic observation of spores, hyphae and septate(?) phialides of various shapes and sizes. These septate hyphae have tiny projections indicating that they used to bear spores and hence part of the fruiting organ.

Rarely I see spores in short chains but often they are very hygrophobic and entrapped in tiny bubbles hindering the view of the conidiaphore+apparatus. Is there a specific mould with this character - breaking out easily? I strongly doubt it is a  Penicillium as I managed to see a fruiting part with budding spore.
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Pintos Angel, 02-12-2017 09:32
Pintos Angel
Re : Mould which easily break when mounted in water or Stains
Looks like Cladosporium sp.

regards

Angel
Joey JTan, 02-12-2017 17:03
Re : Mould which easily break when mounted in water or Stains
Yes this is a Cladosporium sp. The conidial chains easily fragment and can be seen best by making a tape mount. Gently press a small piece of transparent tape against the colony and mount it in water (place it on a drop of water, don't just stick it to the slide itself). You should see the chains still connected. The larger, 2-celled, shield-like ramoconidia are indicative of Cladosporium and give rise to branching conidia chains.
Stephen Mifsud, 07-12-2017 23:29
Stephen Mifsud
Re : Mould which easily break when mounted in water or Stains
Hi thank you for your replies. It took time to come back but I did not forget you. The tape method did a lovely job. I cut a small squarish piece of tape using tweezers and clean scissors, laid the sticky surface on the outer part of the colony, gave a very gentle press (like a touch rather than a press), placed the sticky surface of a drop of water on a mounting slide and mounted under a microscope. It was interesting to observe that this species had clamp junctions. 

Now, I could see the conidiogenesis structure very well and I drew a quick illustration :-) 

I don't think I dare to follow the Cladosporium key of a meticulous monograph to reach species level!
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