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21-10-2021 13:57

Miguel Ãngel Ribes Miguel Ángel Ribes

Buenos díasMe han pasado esta Scutellinia recogid

21-10-2021 15:44

François Bartholomeeusen

Dear forum members,On a rotten piece of wood I fou

21-10-2021 13:26

Chris Yeates Chris Yeates

Bonjour tous While processing images of this deli

21-10-2021 21:09

Enrique Rubio Enrique Rubio

I would be grateful for an opinion on this Orbilia

20-10-2021 08:29

Mirek Gryc

Hello all.I would ask for help in identifying this

21-10-2021 20:14

Ronald Morsink

These i found on the side of a dry creek in the Ne

20-10-2021 21:37

Garcia Susana

Hola, He recogido este ascomyceto que crece de m

10-10-2021 17:35

Marek Capoun Marek Capoun

And one more question from me for today.It was the

18-10-2021 23:10

Jorge Hernanz

Hemos encontrado éste Geoglossum en hábitat alpi

18-10-2021 18:59

Thorben Hülsewig

Hi there,3 days ago I found this ascomycet on leav

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Sub-millmeter whitish apothecia on subalpine humus
Edvin Johannesen, 02-09-2021 13:49
Edvin Johannesen
These tiny apothecia (less than 1 mm) were found in a moist depression under Salix in a mountain region in Central Norway. The substrate is nearly-decomposed plant material.  The macro photos are of fresh specimen. Unfortunately, it took som time (in the fridge) before I was able to do microscopy, so deliquescence has started. I was however able to find mature asci and spores (photos in water). Spores measure typically 11x4 microns. Multiple guttules (or spore bodies?) are seen towards the poles. Asci IKI- as far as I can see. Is it possible to ID (at least to genus) from this limited information?

Thanks.
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Mirek Gryc, 02-09-2021 16:08
Re : Sub-millmeter whitish apothecia on subalpine humus
Hi Edvin

Pezoloma?

Mirek
Edvin Johannesen, 02-09-2021 20:23
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
That's an interesting proposal, Mirek. A genus I have not encountered before. I will check further. Sad to say, the specimen is now in a poor condition.  Thanks!
Hans-Otto Baral, 02-09-2021 22:02
Hans-Otto Baral
Re : Sub-millmeter whitish apothecia on subalpine humus
LBs are for sure in the spores (you can test it with KOH, they resist). But how are the paraphyses? are they still alive and do they contain VBs?
Edvin Johannesen, 03-09-2021 14:34
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
Thanks, Zotto.  I was happy to find one apo apparently in good shape, and have studied it in water, KOH and Lugol. Some additional/corrected information.

1. The spore LBs resist KOH

2. Paraphyses contain VBs. These disappear in KOH and the paraphyses also become thinner in KOH.

3. Asci vary in being uni- or biseriate. Also the number of spores seem to vary, the normal being 8 (uni- or bi). In some asci the spores spread througout the asci, in other they tend to aggregate towards the apex.

4. Ascus apparatus is hemiamyloid (brownish in Lugol).

New photos are all in water (except the last one, in Lugol). I had to use phase contrast to get a clear image of the paraphyse VBs.

Does this help?
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Hans-Otto Baral, 03-09-2021 22:10
Hans-Otto Baral
Re : Sub-millmeter whitish apothecia on subalpine humus
Interesting! Could be a Pezoloma indeed. But I see no VBs. Your phase photo seems to refer to dead paraphyses. In water under bright field there are no apparent contents.
Edvin Johannesen, 03-09-2021 23:43
Edvin Johannesen
Re : Sub-millmeter whitish apothecia on subalpine humus
OK, I learn every day :)
Mirek Gryc, 04-09-2021 07:02
Re : Sub-millmeter whitish apothecia on subalpine humus
Hi
In fact ascomata already heavily damaged but I think the pictures show the contents paraphyses.
I allowed Edvin's photo correction.
Mirek
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