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11-09-2025 16:57

Jason Karakehian Jason Karakehian

Our revision of Marthamycetales (Leotiomycetes) is

10-09-2025 17:18

Blasco Rafael Blasco Rafael

Hola, encontre este estiercol de vaca estos apotec

02-09-2025 11:34

Thomas Læssøe

https://svampe.databasen.org/observations/10527903

10-09-2025 23:53

Marcel Heyligen Marcel Heyligen

Found on Robinia pseudoacasia together with Diapor

07-09-2025 08:19

Josep Torres Josep Torres

Hello.Tiny pinkish discomycetes, photographed and

09-09-2025 12:07

Edmond POINTE Edmond POINTE

Bonjour amis mycologues,Trouvé sur moquette de ch

08-09-2025 19:07

ruiz Jose

Hola me pasan esta recolecta en madera de fraxinus

03-09-2025 21:59

Philippe PELLICIER

La Léchère, Col de la Madeleine, alt 1970m, au s

06-09-2025 21:40

David Chapados David Chapados

Hi, Does somebody know the genus that has those b

07-09-2025 11:34

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello,I have identified this fungus as Hymenoscyph

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Leotiomycetes on Phragmites stem in Ireland
Alden Dirks, 20-08-2024 23:32
Any ideas on what this Leotiomycetes growing on Phragmites stems in Ireland is?
https://www.inaturalist.org/observations/236965918

Unfortunately my desktop computer crashed when I went to plug in a USB stick and the power chord jostled so I don't have any microscopic photos or measurements. But perhaps a general description of the microscopic features is enough for some ideas... 

Spores long, multiseptate, with 9 (?) segments. Amyloid ascus pore. Ectal excipulum of textura prismatica (?).

Tissue was isolated for sequencing.
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Hans-Otto Baral, 21-08-2024 10:10
Hans-Otto Baral
Re : Leotiomycetes on Phragmites stem in Ireland
Quite impossible to say. 9-celled spores would be extraordinary, however. Any idea of the length/width relation? Ascis uodine reacion blue or red? The ectal excipulum I would have expected to be more of globose-angular cells. Anything about parahyses?
Alden Dirks, 21-08-2024 18:27
Re : Leotiomycetes on Phragmites stem in Ireland
Still super bummed about losing all the work I did yesterday but here are a few more photos to continue the discussion. 


Paraphyses about 3.5 µm wide at tips, slightly clavate. Ascospores 35-52 µm long by 4-5 µm wide, with 8 or so septa (not entirely sure), each segment usually with a single large guttule. Asci about 100 µm long by 13 µm wide. I think they might have croziers. Ascus pore blue (amyloid in Melzer's reagent). Yes, you are right on the ectal excipulum - globose-angular cells.
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Hans-Otto Baral, 21-08-2024 20:30
Hans-Otto Baral
Re : Leotiomycetes on Phragmites stem in Ireland
The apical ring should better be tested in Lugol (IKI), and especially a photo of the ring would be valuable. The paraphyses might have contained refractive vacuoles (VBs), but this is now quite uncertain. A possibility would be the genus Niptera. But N. macrospora has 2-spored asci.
Alden Dirks, 21-08-2024 21:06
Re : Leotiomycetes on Phragmites stem in Ireland
Thanks for the thoughts! I don't have Lugol's solution so I'll have to test that later. 

At this stage, would there be anything diagnostic with a clearer picture of the apical ring? 

I have tissue isolated into EnA (extract-n-amp) solution and CTAB. It might be a little while before I have access to a molecular lab again, but eventually I'll be able to sequence these little guys.
Alden Dirks, 21-08-2024 21:18
Re : Leotiomycetes on Phragmites stem in Ireland
Something like Belonopsis excelsior or Neobelonopsis sp. seem like possibilities!
Hans-Otto Baral, 21-08-2024 21:36
Hans-Otto Baral
Re : Leotiomycetes on Phragmites stem in Ireland
You are right, Mollisia excelsior would be a good option, the macroscopy (flat apos) fits. Only the spores are usually 3.5-4 µm wide. M. asteroma has narrower spores and more a raised margin.

There is a CBS sequence in GB (ITS+LSU) and another not uploaded ITS sequence is 100% the same.
Alden Dirks, 21-08-2024 23:02
Re : Leotiomycetes on Phragmites stem in Ireland
Excellent, I'm glad we came to a good candidate, and that there are sequence data to confirm our hypothesis when the time comes. Thanks!