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09-12-2025 12:06

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

07-12-2025 16:07

Arnold Büschlen

Hallo, ich habe in einer Moos-Aufsammlung (epiphy

08-12-2025 21:04

Mark Stevens

"Hello everyone,I'm relatively new to microscopy (

08-12-2025 18:59

Lothar Krieglsteiner Lothar Krieglsteiner

.. found by a seminar-participant, I do not know t

08-12-2025 21:18

Buckwheat Pete

Hello everyone, Is it possible to at least approx

07-12-2025 17:43

Malcolm  Greaves Malcolm Greaves

This Helvella was in mixed woodland. Uniform cupul

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

16-03-2014 22:00

Ralph Vandiest Ralph Vandiest

Hello,I found this species a few months ago but ha

08-12-2025 13:39

Thomas Læssøe

https://svampe.databasen.org/observations/10572899

07-12-2025 22:43

Andreas Millinger Andreas Millinger

Good evening, tried to determine with Munks Valsa

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Pezizales --Pyronema?
Ethan Crenson, 30-06-2025 06:57
Hi all,

Another find by a friend yesterday in Brooklyn, NY.  This one on wood chips near an area that burned last November. However, there was no evidence that this collection came directly from the burn site. 

The paraphyses cylindrical and are multi septate, but contain guttules only at the apex. about 2µm wide.

Spores hyaline, smooth, about 15x10µm.

Asci clavate, IKI-, about 70x10µm

Does this look familiar to anyone?

Thanks in advance for your help!

Ethan
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Hans-Otto Baral, 30-06-2025 08:22
Hans-Otto Baral
Re : Pezizales --Pyronema?
By bad memory I would compare Pyronema domesticum. These multiguttulate VBs I also remember. But these VBs appear to be inconsistent, photos in my folder show that.
Valencia Lopez Francisco Javier, 30-06-2025 15:46
Re : Pezizales --Pyronema?
Hola a todos 
Me recuerda al género iodophanus, que también pueden crecer en suelos quemado, solo que iodophanus tiene reacción amiloide en las paredes de las ascas y esta colección es negativa, no se....
Saludos 
Curro
Ethan Crenson, 30-06-2025 17:32
Re : Pezizales --Pyronema?
Zotto and Curro, thank you for your help.  I was about to dismiss the idea that this is Iodophanus because I had determined that the asci were inamyloid. But I looked again after reading a few descriptions of species of Iodophanus.  

I soaked a fruiting body in Lugol's, after having read that the whole hymenium of I. carneus will change to blue within 10-30 minutes.  In 10 minutes I got a very dark color. I'm not sure I would describe it as blue. 

I looked at the asci from that fruiting body after that soaking in IKI to see if they were "diffusely amyloid" (fungi of temp europe) or "turn blue all over" (ascomycetes of NA). It looks like they do! I think I missed the blue color because the liquid contents of the asci became red in the iodine, obscuring the blue color of the ascus walls.

I also tried to determine if the spores were textured at all.  In the Lugol's it looks like they might be faintly textured, but about this feature I am not entirely certain. 
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Hans-Otto Baral, 30-06-2025 21:34
Hans-Otto Baral
Re : Pezizales --Pyronema?
It is actually so that Iodophanus has exactly this kind of VB-guttules in the top cell. I thought I see this obscurely in one of the Pyronema pics, but maybe I am wrong. Usually the IKI reaction is stronger blue. Now I also see the warts on the spores.
Ethan Crenson, 01-07-2025 18:27
Re : Pezizales --Pyronema?
It took me quite a few attempts to see the warts on the spores in cotton blue.
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