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11-11-2024 23:17

B Shelbourne B Shelbourne

• Macro and habitat suggest Hyaloscyphaceae s.l.

13-11-2024 08:01

Stephen Martin Stephen Martin

I am revising some old material again and I have t

10-11-2024 17:09

Malcolm  Greaves Malcolm Greaves

This Geoglossum has 7 septate spores 75-90 long. T

09-11-2024 16:41

Stephen Martin Stephen Martin

Hello everyone, we have recently published a paper

11-11-2024 14:05

Edouard Evangelisti Edouard Evangelisti

Bonjour le forum, Je sollicite votre avis concern

10-11-2024 19:47

B Shelbourne B Shelbourne

• Macro and habitat suggest Phaeohelotium, confi

08-11-2024 17:36

Juuso Äikäs

Recently I posted here my finding of small white a

08-11-2024 16:54

David Chapados David Chapados

Hi, I'm looking for help to identify this specime

08-11-2024 15:44

Laurent LENEL Laurent LENEL

Bonjour à tous, ma découverte du jour, une Helve

23-10-2024 17:27

Enrique Rubio Enrique Rubio

Hi againThese tiny apothecia (100-200 µm) were gr

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Hyaloscypha aureliella on Pinus sylvestris
B Shelbourne, 11-11-2024 23:17
B Shelbourne• Macro and habitat suggest Hyaloscyphaceae s.l.
• Hyaloscypha suggested by hairs and spores.
• Spores, asci, hairs, and substrate, seem to suggest H. aureliella.
• Subiculum and conidia look similar to photos of CBS 126298.

Habitat: On the underside of two pieces of wood from Pinus sylvestris, a part of a small branch and a broken part of a larger piece of wood, mostly on decorticated parts, damp when found, on the floor, grassy and exposed locations, 175 m alt., Old Lodge nature reserve, Ashdown Forest, High Weald, southern England, early-November.

Apothecia: Apparently sometimes arising from a +/- dense subiculum, could be an anamorph, whitish to pinkish-orangish to brownish, appearing downy or foam-like with hyphae difficult to distinguish, gregarious to 6+-caespitose, many 10s of apothecia on the larger piece, diameter (0.35) 0.40-0.53-0.75 (0.93) mm, sessile, superficial, whitish-translucent with some hints of orangish-brown.

Low magnification: Cupulate to slightly urceolate, eventually more discoid, receptacle and margin whitish with many short hairs, often with a moderate number of large drops of golden-brownish exudate around the flanks, disc concave, more translucent with a gelatinous appearance, margin round or occasionally lobate, often compressed by the environment, hairs noticeable and more reflective, apparently remaining raised above the disc.

Asci: Turgid ~54-63 x 7-8 µm, croziers, rings bb, more like Calycina-type?

Spores: Ellipsoid-clavate, several small LBs towards each pole, OCI < 1, asymmetric or sometimes slightly curved in profile view.

Free spores in water or in mature asci:
(8.2) 8.4-9.6 (10.3) × 2.2-2.6 µm, Q = (3.3) 3.4-4.0 (4.4), N = 23, mean = 9.0 × 2.4 µm, Q mean = 3.8.

Paraphyses: Apparently few, filiform, apex not to slightly inflated, width ~2-3 µm, septate, no notable contents.

Hairs: Protruding part ~45-55 (60) x 3.5-4.0 µm, flexible, tapering to a rounded end, aseptate, hyaline, some agglutinated with very large drops of oily golden exudate, leaving many small globules on the hairs, one or two globose to sub-globose basal cells.

Medullary: Not seen clearly, possibly reduced.

Ectal: Text. prismatica to globosa-angularis, many small patches of amyloidity.

Conidia: Stuck to the receptacle, light brown walls, all seen with two globose cells, one appears to be developing another septum, septal pore often noticeable.
  • message #80645
  • message #80645
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Hans-Otto Baral, 12-11-2024 09:11
Hans-Otto Baral
Re : Hyaloscypha aureliella on Pinus sylvestris
For sure. The conidia I do not remember. What is the link to the CBS sample?
B Shelbourne, 12-11-2024 09:27
B Shelbourne
Re : Hyaloscypha aureliella on Pinus sylvestris
There is a figure in your folder (https://drive.google.com/file/d/1RfRKdt0KgJYIKcH83L5Fp7pe1vsFSXly/view?usp=drive_link), with the citation Fehrer et al. 2018. I think this is the paper showing Meliniomyces/Rhizoscyphus is in Hyaloscypha.
Hans-Otto Baral, 12-11-2024 09:48
Hans-Otto Baral
Re : Hyaloscypha aureliella on Pinus sylvestris
Ah o.k., these 3-4-celled and partly branched conidia. I never saw them in nature.
B Shelbourne, 12-11-2024 10:20
B Shelbourne
Re : Hyaloscypha aureliella on Pinus sylvestris
It may be unrelated, but some of the apothecia seem very integrated with the mycelial mat and there are some similarities with the appearance of the culture and conidia.

I am also reminded that Kosonen et al. recommended promotion of subgenus Eupezizella, defined mostly by aseptate hairs with resinous exudates.


B Shelbourne, 14-11-2024 11:04
B Shelbourne
Re : Hyaloscypha aureliella on Pinus sylvestris
I had another look at the substrate and there are many fungi coinhabiting, so I think the conidia probably belong do a different fungus. I also sampled an area of thick orangish mycelium and it appears to be (mostly) a corticoid basidiomycete. There are some very large hyphae without clamps and some smaller hyphae at the edges with clamps.
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