Hi Roland

do you have this find only in the dry state? There are surely paraphyses, and I suspect this is a Mollisia. Living paraphyses would be required to confirm this, although it seems to me quite clear.

Zotto

Hi Zotto !

Yes, we worked only in dry state. The specimens were very brittle.
No paraphyses were found in spite of many observations.
They might be present, but we were unable to find them.
We passed a lot of time to observe this Disco.

Do you have a suggestion for and indentification ? In the Mollisia ?

Thank you Zoto, it's a great pleasure to contact you again !

Roland

You could add KOH during looking through the microscope at 100-400x, some species of Mollisia get yellow for a moment thereby. In KOH you should find paraphyses, if it is a Mollisia.

To identify species of Mollisia is quite difficult. Perhaps it is M. benesuada. You can also try the key of Andreas Gminder.

http://www.mollisia.de/start.html

Zotto

Hi Zotto !

We have looked for paraphyses and it's seem again that are'nt.
There is no yellow reaction with KOK, the color being the same as H2O, ochraceus.
More, we don't see hairs on outside of cup nor on margin.

I tried the Andreas's key on Mollisia.
Too much details are missing for us to get further.

Friendley Hans ! Roland

Bonjour,

j'ai obtenu un exsiccatum par Jacqueline Labrecque avec trois apothecia.

Ma examination a rélévé presque les mêmes dates que Roland a deja donné:
Asques appr. 105 x 5 µm (toutes mortes), avec croziers, réaction au Barals intensement bleu.
Spores 11-15 x 2,5-3 µm (toutes mortes), souvent curvé (reniforme), avec des pols +/- attenué, avec beaucoup de contenu huileux (appr. 4 d'après BARAL), confluent en 3-5 grosses guttules, sans cloissons dans les asques, mais souvent cloisonnées dehors les asques (1-3 septes).
Paraphyses cylndrique, appr. 2-2,5 µm en largeur, sans contenu ou avec quelques diffuses restes de content huileux.
KOH réaction negativ.
Pas de subiculum, mais ca et la quelques hyphes subiculaire, banal
Cellules marginals, banal, brunâtre.

Tout ca juste parfaitement pour Mollisia ventosa. L'image au dessus juste assez bien aussi. A mon avis il n'est pas trop hazardeuse de baptisé cet recolte comme Mollisia ventosa.

amicalement,
Andreas

Merci Andreas !

Votre qualité et précision d'étude nous aident énormément.

Amitiés,
Roland

Hi Roland & Andreas

now I understand the image of the non-septate dead spores: the plasma is stained blue while the hyaline regions correspond to the oil drops which do not stain in such blue basic dyes.


Zotto