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Margot en Geert Vullings

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Hello.An anamorph located on the surface of a thin

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Malcolm  Greaves Malcolm Greaves

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Hi all,We found picnidia on Protoparmeliopsis mur

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Hardware Tony Hardware Tony

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difficult inoperculate Discomycete
Uwe Lindemann, 06-06-2010 00:11
Uwe LindemannHello Forum,
I recently found an inoperculate discomycete which I can’t determine. I think it’s similar to Psilocistella quercina but I am not sure.
Here are some data:

Macro
Apothecia up to 0,3 mm, whitish-brownish
on wet, rotten wood of a broadleaf tree, between Hyaloscypha albohyalina var. albohyalina (see the red markers)

Micro
Asci 8sp, with croziers, IKI+ b, 38-43 x 6-7,5 µm
Paraphyses filiform, not pointed, no content
Ascospores hyalin, with two guttules, 5-6 x 3 µm
Ec. Excipulum yellowish-brownisch T. intricata
Medulla yellowish-brownisch T. angularis
Hairs ("marginal cells") smooth, yellowish-brownisch, up to 45 µm

I hope that you can help me.
Best,
  • message #11799
Uwe Lindemann, 06-06-2010 00:12
Uwe Lindemann
Re:difficult inoperculate Discomycete
micro
  • message #11800
Hans-Otto Baral, 06-06-2010 13:11
Hans-Otto Baral
Re:difficult inoperculate Discomycete
Hi Uwe

I need to see the hairs. Are they cylindrical, septate? Width?

What is the substrate?
Zotto
Uwe Lindemann, 06-06-2010 13:25
Uwe Lindemann
Re:difficult inoperculate Discomycete
Hi Zotto,
sorry, I am not precise enough in my description. You can see the hairs on the micr photo left above. The hairs are cylindrical, septate, width 2,5-3 µm.
The substrate is, as I wrote above, very wet and rotten wood of a broadleaf tree, maybe Quercus.
Best, Uwe
Hans-Otto Baral, 06-06-2010 16:47
Hans-Otto Baral
Re:difficult inoperculate Discomycete
Hi Uwe

sorry for being so blind! Well, yes, the hairs are well visible. And the excliple is a brownish angularis (I think you mixed ectal and medullary). This both looks like a Pyrenopeziza. The spores remind me of our P. ulicis nom. prov., but there the spores get warted. Spore size fits well and a bit hairs are also present there (DVD: HB 6600, JPP 22105).

Zotto
Uwe Lindemann, 06-06-2010 17:31
Uwe Lindemann
Re:difficult inoperculate Discomycete
Oh, yes, P. ulicis looks quite similar.
I don't see warts on the spores, but the warts are maybe too little to see it with my technical equipment.
Thank you again very much for your help!
Uwe
Hans-Otto Baral, 06-06-2010 18:37
Hans-Otto Baral
Re:difficult inoperculate Discomycete
On your photo the spores look a bit warted. This might be due to not enough resolution, but I think they mean a rough surface. The warts in P. ulicis are not very prminent. Perhaps you can stain them with cresylblue? I think a normal microscope is able to resolve them :-) And if a photo shows them, then direct viewing should even more be possibe. The warts appear to exist only very late in development. I wrote: inside living asci they look +/- smooth.

Zotto
Uwe Lindemann, 06-06-2010 22:02
Uwe Lindemann
Re:difficult inoperculate Discomycete
I think it is due to the bad resolution that you saw warts ;-) In higher resolution (see photo) the spores are almost smooth except (maybe) the marked areas.
Thanks a lot for your additional explanations!
Uwe
  • message #11821
Hans-Otto Baral, 06-06-2010 22:35
Hans-Otto Baral
Re:difficult inoperculate Discomycete
O.k.

can you give me an idea of the kind of forest. We had this P. ulicis only on Ulex, but in Spain it was found on Eucalyptus :-) by Javier Fernandez, also with nicely warted spores.

Zotto
Uwe Lindemann, 06-06-2010 23:01
Uwe Lindemann
Re:difficult inoperculate Discomycete
The find is from a fieldtrip last thursday - with Klaus, Fredi and Dirk ;-) - to the NSG Davert near Münster. It's an mixed (overgrown) forest with different broadleaf trees like Acer, Salix, Alnus, Quercus, Carpinus, Fagus and Ulmus. The place where I found it: http://maps.google.de/maps?f=d&source=s_d&saddr=51.852958,7.595973&daddr=&hl=de&geocode=&mra=dme&mrcr=0&mrsp=0&sz=15&sll=51.853435,7.600608&sspn=0.011663,0.020728&ie=UTF8&ll=51.852408,7.59578&spn=0.002916,0.00927&t=h&z=17
See also here: http://www.davensberg.de/geschichte/inf_archiv_0019.htm
Uwe