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Hi! I found this interesting tiny ascomycete on i

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Sebastien BassoHello, I'm conducting a mycological inventory in
[Lachnum cf. reynoutriae] Lachnum subvirgineum
Yannick Mourgues,
25-06-2010 00:50

Here is e collection of lachnum cf. subvirgineum found on dead stalks of Epilobium.
spores 8,5-10x1,9-2,3 um with very small guttules at each end not always visible.
asci IKI+ (Lugol) 50-60x4-5 um.
paraphyses lanceolate, without any content, 20-30 um protruding, 65-80x5-6 um.
Marginal hairs 100-110x4-5 um.
I think it's L. subvirgineum.
Do you agree with me ?
Yannick
Hans-Otto Baral,
25-06-2010 08:44

Re:Lachnum subvirgineum
Hi Yannick
The hair apices are not swollen enough. I think about Lachnum "reynoutriae" which I had once on other herbs than Renoutria. This needs to check the croziers which should be not that difficult in Congo. Your statement on empty paraphyses refers to living material? Du you also have photos of the living?
Zotto
The hair apices are not swollen enough. I think about Lachnum "reynoutriae" which I had once on other herbs than Renoutria. This needs to check the croziers which should be not that difficult in Congo. Your statement on empty paraphyses refers to living material? Du you also have photos of the living?
Zotto
Yannick Mourgues,
25-06-2010 23:46

Re:Lachnum subvirgineum
Hi Zotto.
Thank's again !
Here are pictures of ascis from rehydrated materials. Unfortunately, all is dry.
I have checked paraphyses again and found just one with 5 guttules... But I remember I saw a small number of paraphyses with a few guttules among the others when I examined this recolt last day.
Yannick
Thank's again !
Here are pictures of ascis from rehydrated materials. Unfortunately, all is dry.
I have checked paraphyses again and found just one with 5 guttules... But I remember I saw a small number of paraphyses with a few guttules among the others when I examined this recolt last day.
Yannick
Yannick Mourgues,
25-06-2010 23:49
Hans-Otto Baral,
25-06-2010 23:53

Re:Lachnum subvirgineum
O.k., then it is probably L. reynoutriae nom. prov. Because L. virgineum and subvirgineum have asci with croziers.
For the VB-guttules you need living paraphyses. Lachnum species are usually not inert to drying. Don't use Congo or anything else when looking for vital characters. :-) For croziers Congo is wonderful.
Zotto
For the VB-guttules you need living paraphyses. Lachnum species are usually not inert to drying. Don't use Congo or anything else when looking for vital characters. :-) For croziers Congo is wonderful.
Zotto
Yannick Mourgues,
25-06-2010 23:56

Re:Lachnum subvirgineum
Considering the dimension of spores, is Lachnum subrenoutriae a better candidate ?
Yannick
Yannick
Hans-Otto Baral,
26-06-2010 00:12

Re:Lachnum subvirgineum
pfff, I do not know that species :-) It is a very old name of mine, which I attached to a single collection on unidentified substrate, apparently wooden. I have no illustration, only some notes on microscopy.
Spore length was very variable in this find, 6-12 µm. I fear I need to restudy this find at some time.
A week ago I received fresh material of L. reynoutriae, on Reynoutria (Fallopia) japonica. The data:
Chemnitz, Reynoutria. Asci *41-47 x 4.3-4.7 µm, H- (2 ap. tested), IKI 3bb. Sp. *5.5-8 x 1.6-1.8 µm, base mostly acute, Ölm-0-0-5. Hairs 50-90 µm, apex 0-1inflated, (2-)3-celled. Paraph. 0-1/2refr. VBs multigutt., in IKI strongly refr.
So the spores are indeed quite short in that species. There were actually very indistinct VB-guttules present in water, which became strongly visible in IKI (vital staining).
Zotto
Spore length was very variable in this find, 6-12 µm. I fear I need to restudy this find at some time.
A week ago I received fresh material of L. reynoutriae, on Reynoutria (Fallopia) japonica. The data:
Chemnitz, Reynoutria. Asci *41-47 x 4.3-4.7 µm, H- (2 ap. tested), IKI 3bb. Sp. *5.5-8 x 1.6-1.8 µm, base mostly acute, Ölm-0-0-5. Hairs 50-90 µm, apex 0-1inflated, (2-)3-celled. Paraph. 0-1/2refr. VBs multigutt., in IKI strongly refr.
So the spores are indeed quite short in that species. There were actually very indistinct VB-guttules present in water, which became strongly visible in IKI (vital staining).
Zotto
Yannick Mourgues,
26-06-2010 00:21

Re:Lachnum subvirgineum
Ok Thank's !
I have another one for you... !
Yannick
I have another one for you... !
Yannick