When studying them I noticed a very big spore and thought, that its origin must be somewhere else, because with so big spores those asci I saw could be only 2.spored.
Suprisingly it was so.
The spores were about 30-40x5-7 and seemed to have sometimes 1 or 3 septas.
Asci were 60-85x9-12, IKI+.
The paraphyses were septate, branching and with irregulary swollen tips.

Heres one more photo.

I tried to find something, which would fit, but with no success...

Marja

Never heard of such a fungus. To clarify needs some more features:

- I can notice globose almost hyaline cells of the excipulum, anyhow, the fungus is sessile and should quite easily be sectioned with a razor blade. A view on a median section of the ectal excipuöum would surely help, on flanks and on margin.

- Oil immersion images of the spores and their contents. Are they non-septate inside the living asci?

- IKI reaction blue or red, how is the shape of the apical ring in dead asci, photo possible?

Nimbomollisia macrospora is the only I can guess, oh yes, it is, it has 2-spored asci! See f.ex. Schmid-ascomyceten im Bild 1&2_Page_145.jpg

The fungus is erumpent, if you dry them they should retract and get covered by lobes of epidermis.

Despite my identification please try to answer my questions because I feel that nobody ever described the vital features of this species, including myself.

Zotto

Oops,

are you asking me to start shaving ;)
I see, what I can do, don't expect much.
Here's something from last night:

And one more.

I think I saw a spore with 1 septa inside the ascus, but I was far too tired last night to see anything clear...
I don't know the paper you mentioned, where can I find it?

So that I was hoping to go picking some edible mushrooms,,,
But the specimen can wait in fridge :)

Marja

your joke I did not understand :-(

The asci are very nice, fully alive, and in the upper I can see septa (not in the lower because of the disturbing scale...)

In IKI these are turgescent still which means that the apical wall is compressed and the ring shape not visible. In Schmi the ring shape is visible, so what I manly wish to know here is the colour, blue or red or something between.

Zotto

Blue, blue, electric blue in my eyes.

Marja

Sorry,
I've got no preparation microscope nor three hands.
One is needed for the loupe, another for the blade and third for some needle or like that to take the pieces of the blade ;)
Anyway here's something.

Spore

Asci in Lugol,
It is a constant fight to get microphotos with my camera.
It just won't focus before tens and tens of trials and then the quality is like this:

Its quite good, thanks. I know the problem with focussing. With my Coolpix I need to see the object unsharp through the microscope ocular in order to get sharp photos! This is the opposite of confocal. But among 5-10 photos is usually a sharp one :-) Recommended is to switch out the automatic focus.

For making sections you need perhaps a "third hand", a apparatus which holds the lens. Of course a dissection microscope would be much superior.

Zotto

here is the Schmid pages

Thanks :)

Even I can see, that at least my macro is actually much better than those on the Schmid pages ;)

Once again this seems to be a Petter Adolf Karsten-species.

Marja

Do you have a higher resolution for your macro? I like it very much but it is a bit small.

P.S Schmid simply failed to better rehydrate the specmien. Or he should have made one dry and one rehydrated.

Zotto@arcor.de

Dear Zotto,

if there is something I can do for you, I'll do it, if I can ;)
I'll send you original photos later, now I got to run.

Marja