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Chlorociboria.. .
Martin Bemmann,
17-10-2010 22:38
Hi all,I collected a broken twig of possibly Fagus (according to te remainig bark), stained green by a Chlorociboria. Under the dissecting microscope it featured some "black" fruiting bodies resembling to Bertia moriformis by shape. They appeared always allong the green stained portions of the wood and were soft, gelatinous when cut and featured lots of green pigments as well. Ass. on other parts of this twig were Calocera cornea and a yet unidentified discomycete (my macroscopical guess is a Hyalorbilia).
Can somebody of you give a hint to literature for the anamorph or is this a sclerotium of C.?
Attached are some pictures that could be better.
Best regards,
Martin
Martin Bemmann,
17-10-2010 22:40
Re:Chlorociboria.. .
sclerotia...anamorphs...?
Martin Bemmann,
17-10-2010 22:40
Re:Chlorociboria.. .
sclerotia...anamorphs...? pt2
Martin Bemmann,
17-10-2010 22:41
Re:Chlorociboria.. .
Conidia..?
Martin Bemmann,
17-10-2010 22:41
Re:Chlorociboria.. .
Conidia pt.2
Martin Bemmann,
17-10-2010 22:42
Re:Chlorociboria.. .
Conidia pt.3
Martin Bemmann,
18-10-2010 11:04
Re:Chlorociboria.. .
Hello again,
my fungus seems to be Dothiorina tulasnei, an anamorphic fungus in discussion to be related to Chlorociboria. Though Sánchez & Bianchinotti (Mycotaxon 102, 395-402) are raising doubts (p.401). Reportedly there was no successful culture made of Ch. yet.
Best regards,
Martin
my fungus seems to be Dothiorina tulasnei, an anamorphic fungus in discussion to be related to Chlorociboria. Though Sánchez & Bianchinotti (Mycotaxon 102, 395-402) are raising doubts (p.401). Reportedly there was no successful culture made of Ch. yet.
Best regards,
Martin
Martin Bemmann,
19-10-2010 01:06
Re:Chlorociboria.. .
Some more pictures as I knew now what to look at..
first a little better macro:
first a little better macro:
Martin Bemmann,
19-10-2010 01:07
Re:Chlorociboria.. .
a section with loculi:
Martin Bemmann,
19-10-2010 01:07
Re:Chlorociboria.. .
larger:
Martin Bemmann,
19-10-2010 01:09
Re:Chlorociboria.. .
Conidiophores:
Martin Bemmann,
19-10-2010 01:12
Re:Chlorociboria.. .
and conidia (3.6-5.3 x 0.8-1.06 µm)
Has somebody of you experienced this fungus?
Best regards,
Martin
Has somebody of you experienced this fungus?
Best regards,
Martin
Hans-Otto Baral,
19-10-2010 09:03
Re:Chlorociboria.. .
Hi Martin
that's wonderful, and it resembles a bit the Coryne anamorph of Ascocoryne. Never seen. I have this ana-teleo relation in my database, but saw it only in Dixon 1975.
Zotto
that's wonderful, and it resembles a bit the Coryne anamorph of Ascocoryne. Never seen. I have this ana-teleo relation in my database, but saw it only in Dixon 1975.
Zotto
Christian Lechat,
19-10-2010 10:14
Re:Chlorociboria.. .
Hi Martin,
are cells inside of the locule become green in KOH and conidia formed in chains?
If so, then I think your fungus is probably Dothiorina tulasnei.
Regards,
Christian
are cells inside of the locule become green in KOH and conidia formed in chains?
If so, then I think your fungus is probably Dothiorina tulasnei.
Regards,
Christian
Hans-Otto Baral,
19-10-2010 11:36
Re:Chlorociboria.. .
I think I now see that the conidia pass a rather long and narrow collarette, do they?
I did not note a reaction of the green pigment of Chlorociboria in KOH, only a dissolution of the exudate which turned yellowish-greenish, but not staining the medium (Chl. aeruginoa).
I did not note a reaction of the green pigment of Chlorociboria in KOH, only a dissolution of the exudate which turned yellowish-greenish, but not staining the medium (Chl. aeruginoa).
Martin Bemmann,
19-10-2010 14:55
Re:Chlorociboria.. .
Hello Zotto and Christian,
I made a new section in order to see better the inner structure of the loculi. The conidiophores are arranged all along the inner walls with their necks upwards. And yes, Zotto, the conidia are produced in chains inside a collarette. Hope one can see that.
KOH has a bleaching effect turning the bright turcoise into olive-brown.
Best regards,
Martin
I made a new section in order to see better the inner structure of the loculi. The conidiophores are arranged all along the inner walls with their necks upwards. And yes, Zotto, the conidia are produced in chains inside a collarette. Hope one can see that.
KOH has a bleaching effect turning the bright turcoise into olive-brown.
Best regards,
Martin
Martin Bemmann,
19-10-2010 14:56
Re:Chlorociboria.. .
Loculi:
Martin Bemmann,
19-10-2010 14:57
Re:Chlorociboria.. .
and larger:
Martin Bemmann,
19-10-2010 14:57
Re:Chlorociboria.. .
conidiophores:
Martin Bemmann,
19-10-2010 14:58
Re:Chlorociboria.. .
reaction in KOH 3%:
MARIA VIRGINIA BIANCHINOTTI,
19-10-2010 15:07
Re:Chlorociboria.. .
Hi Martin, nice photos!
I also believe this is Dothiorina tulasnei
I´m sending a pdf file.
best regards
Virginia
I also believe this is Dothiorina tulasnei
I´m sending a pdf file.
best regards
Virginia
13062.pdf
Martin Bemmann,
19-10-2010 15:17
Re:Chlorociboria.. .
Thank you very much, Virginia!
As I understood from your paper, you don't belive, that Dothiorina is related to Chlorociboria?
Best regards
Martin
As I understood from your paper, you don't belive, that Dothiorina is related to Chlorociboria?
Best regards
Martin
MARIA VIRGINIA BIANCHINOTTI,
19-10-2010 15:29
Re:Chlorociboria.. .
yes, I don´t, but to confirm this it would be necessary to do molecular studies, Your material seems fine to attempt isolating, have you tried?
best regards
Virginia
best regards
Virginia
Martin Bemmann,
19-10-2010 15:33
Re:Chlorociboria.. .
No this is far beyond my capabilities! But I can send you material, if you are interested (you may send your postal address to martin.bemmann@gmx.de ).
Regards
Martin
Regards
Martin
Hans-Otto Baral,
19-10-2010 17:52
Re:Chlorociboria.. .
That's very interesting. The macro in your paper, Virginia, does not look very much like Martin's fungus, and the corticated branch is very different from Chlorociboria substrate as we know it from Europe. Do you remember, was the substrate stained bluegreen inside?
Does anybody have a pdf of this paper?
Johnston PR, Park D. 2005. Chlorociboria (Fungi, Helotiales) in New Zealand. New Z. J. Bot. 43:
679-719.
Zotto
Does anybody have a pdf of this paper?
Johnston PR, Park D. 2005. Chlorociboria (Fungi, Helotiales) in New Zealand. New Z. J. Bot. 43:
679-719.
Zotto
Martin Bemmann,
19-10-2010 18:23
Re:Chlorociboria.. .
Hi Zotto,
You can download here:
http://www.royalsociety.org.nz/publications/journals/nzjb/2005/043/
Grüße,
Martin
EDIT: this may work...
You can download here:
http://www.royalsociety.org.nz/publications/journals/nzjb/2005/043/
Grüße,
Martin
EDIT: this may work...
MARIA VIRGINIA BIANCHINOTTI,
19-10-2010 18:27
Re:Chlorociboria.. .
Hi Zotto, perhaps Martin´s material is younger or more fresh than ours. Our material was collapsed. Our branch is Nothofagus pumilio.
The substrate wasn´t stained bluegreen, but logs with Chlorociboria were stained bluegreen (we have also seen Chlorociboria on the same host).
I´m sending you the paper to your mail
Virginia
The substrate wasn´t stained bluegreen, but logs with Chlorociboria were stained bluegreen (we have also seen Chlorociboria on the same host).
I´m sending you the paper to your mail
Virginia
Alex Akulov,
02-10-2011 11:20
Re : Chlorociboria.. .
Dear Friends
Among the samples from the Ukrainian Carpathians I also found Dothiorina tulasnei. The fungus formed sporulation on fallen decorticated branches of Fagus, which were coloured by Chlorociboria (see photo below).
In my sample, the conidia are slightly different in size from those that have been described by Virginia. Their length is 3,4-5,6 (6,5), width - 0.9-1.1 (1.2) micron.
If anyone interested, I could send samples for more in-depth research.
Thank you for the wonderful discussion on the forum!
It helped me to understand with what I am dealing with.
Alex
Martin Bemmann,
02-10-2011 19:52
Re : Chlorociboria.. .
Hi Alex,
wonderful collection! Keep it aside! I am still waiting for a branch with both the ana-and teleomorph together as in the Tulasnes plate... ;-)
Cheers,
Martin
wonderful collection! Keep it aside! I am still waiting for a branch with both the ana-and teleomorph together as in the Tulasnes plate... ;-)
Cheers,
Martin