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Pale Nectria on Fagus
Martin Bemmann, 29-11-2010 00:14
Martin BemmannHi all,
I know, Nectriaceae are very difficult (and that is more or less all I know about N. ;-) ). But these Nectrias maybe have some features that may allow someone of you to determin them:
I found them on a sturdy fragment (ca. 12-15 cm dm) of a Fagus trunk with whiterot, partly decorticated. Other fungi were Orbilia delicatula/leucostigma and Rhizodiscina lignyota.
The tiny (0,2 mm dm.) perithecia are embedded in a white subiculum. Colour is pale orange, older specimen are broken or collapsed. The ascus wall seems to be very thin, since it was almost unvisible to me. Spores are one to mostly two celled with a sometimes asymmetrically shape (5,3-6,5 x 3,1-4,1 µm). Paraphyses are filiform with small oildrops.
Any comments appreciated!
I will try to keep the specimens allive for further investigation.

Best regards,

Martin

PS: reminds me to nougat de Montelimar...
  • message #13568
Martin Bemmann, 29-11-2010 00:15
Martin Bemmann
Re:Pale Nectria on Fagus
another view:
  • message #13569
Martin Bemmann, 29-11-2010 00:18
Martin Bemmann
Re:Pale Nectria on Fagus
a perithecium:
  • message #13570
Martin Bemmann, 29-11-2010 00:20
Martin Bemmann
Re:Pale Nectria on Fagus
its content:
  • message #13571
Martin Bemmann, 29-11-2010 00:21
Martin Bemmann
Re:Pale Nectria on Fagus
Asci and spores:
  • message #13572
Martin Bemmann, 29-11-2010 00:22
Martin Bemmann
Re:Pale Nectria on Fagus
some more spores:
  • message #13573
Martin Bemmann, 29-11-2010 00:22
Martin Bemmann
Re:Pale Nectria on Fagus
and paraphyses in IKI:
  • message #13574
Christian Lechat, 29-11-2010 06:48
Christian Lechat
Re:Pale Nectria on Fagus
Hi Martin,
maybe your fungus is a Nectriopsis, it would be necessary to test the reaction in KOH.
Besides, I would like to know if the hyphae covering ascomatal wall are spinulose or smooth.
Finaly, could you make a vertical section through the ascomatal wall?

Best wishes
Christian
Martin Bemmann, 29-11-2010 22:50
Martin Bemmann
Re:Pale Nectria on Fagus
Hi Christian,

thank you for your interest!
As for your question to the surface of the hyphae, they are not smooth but warty spinulose.
What do you expect, what should I observe, in KOH at what magnification? A change of colour of the perithecium?
Well a section of these very tiny fungi will be a real challenge, but I will try...

Cheers,

Martin
  • message #13595
Martin Bemmann, 30-11-2010 00:11
Martin Bemmann
Re:Pale Nectria on Fagus
Hi again,

this is a comparision of colour in water and KOH 3%. No major change...
  • message #13596
Martin Bemmann, 30-11-2010 00:38
Martin Bemmann
Re:Pale Nectria on Fagus
A last time for today:

I embedded some specimen in PEG and tried to make some random sections. Well I succeeded halfways ... Here is the ascomatal wall to your evaluation.

Best regards,

Martin
  • message #13598
Christian Lechat, 30-11-2010 06:48
Christian Lechat
Re:Pale Nectria on Fagus
Hi Martin,
according to the characters that you provide, such not changing color in KOH, ascospores size and subiculum composed of spinulose hyphal elements, I think it could be Nectriopsis oropensoides (Rehm) Samuels. You should look for in this genus to confirm possibly this hypothesis.

best whishes

Christian
Martin Bemmann, 30-11-2010 10:05
Martin Bemmann
Re:Pale Nectria on Fagus
Dear Christian,

thank you very much. I don't have access to Gary Samuels description 1988. But I found the "Key to Tropical Species of Nectria-like Fungi" (Samuels/Rossman/Schroers 2002) where it is keyed:

4. Perithecial hairs cylindrical, hyphal; ascospores 5.5-7 x 2.5-3.5 µm................Nectriopsis oropensoides (Rehm) Samuels (Samuels, 1988a)

Could it be that the "paraphyses" I showed above are such perithecial hairs? Or where do I have to search them?

Regards,

Martin
Christian Lechat, 30-11-2010 12:50
Christian Lechat
Re:Pale Nectria on Fagus
here is the description from Gary 1988
Martin Bemmann, 30-11-2010 13:09
Martin Bemmann
Re:Pale Nectria on Fagus
Thank you very much!
The description fits well to the features I could observe. I saw as well the entry in the AscoFrance-database. The spores of this collection look more cylindrical then those in mine. But this evening I will make a new slide in CB to document the verrucous surface of the spores which is only faintly visible in my water mount.

Best regards,

Martin
Alex Akulov, 30-11-2010 21:43
Alex Akulov
Re:Pale Nectria on Fagus
Let me to send you the description and illustrations of Nectriopsis oropensoides from Samuels, 1997 (Identification workshop on the Hypocreales. Sugadaira Montane Research Centre, Mycological Society of Japan)
  • message #13625
Martin Bemmann, 30-11-2010 23:58
Martin Bemmann
Re:Pale Nectria on Fagus
Thank you Alex for this !
I tried to document the spore surface but in vain. Only empty ascomata today. At least I could shoot a picture of the subiculum hyphae in CB...
Best regards,
Martin
  • message #13626
Martin Bemmann, 02-12-2010 00:17
Martin Bemmann
Re:Pale Nectria on Fagus
Hi again,

I did not manage to receive CB pictures of the spore surface again. But I think, one can imagine the roughness of the spore surface even in the water mount.

Best regards,

Martin
  • message #13650
Martin Bemmann, 02-12-2010 00:17
Martin Bemmann
Re:Pale Nectria on Fagus
another:
  • message #13651