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17-02-2013 08:38

Alain GARDIENNET Alain GARDIENNET

Bonjour, J'ai trouvé ces acervules sur feuille d

21-12-2024 09:08

Castillo Joseba Castillo Joseba

Me mandan el material seco de Galicia,  recolecta

21-12-2024 11:14

Michel RIMBAUD

Hello,Does somebody could send me a key for Olla/U

21-12-2024 12:45

Marc Detollenaere Marc Detollenaere

Dear Forum,On naked wood of Fagus, I found some ha

17-12-2024 12:33

Lothar Krieglsteiner Lothar Krieglsteiner

this fluffy anamorph was repeatedly found on decid

20-12-2024 20:30

Bernard CLESSE Bernard CLESSE

Bonsoir à toutes et tous,Pourriez-vous m'aider à

19-12-2024 09:27

Castillo Joseba Castillo Joseba

Llevo mas de 6 meses que no puedo contactar con Re

20-12-2024 00:01

Francois Guay Francois Guay

Hi, I found this species on incubated Fir needles

20-12-2024 20:34

Enrique Rubio Enrique Rubio

Perhaps some of you can help me identify this smal

18-12-2024 10:05

ruiz Jose antonio

Buenos días a todos, un pequeño ascomiceto de un

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!