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30-03-2026 12:03

William Slosse William Slosse

Hello all,On 27/03/26, in Kraaiveld in Wingene (Be

30-03-2026 12:18

Sylvie Le Goff

BonjourRécolté sur la base de Pteridium aquilinu

25-03-2026 10:35

Hulda Caroline Holte

Hello,I collected this species growing on a dead b

28-03-2026 17:41

Louis DENY

Bonjour forum,Mollisia trouvée sur tige de Molini

30-03-2026 09:53

Yanick BOULANGER

BonjourVoici des petites fructifications poilues s

27-03-2026 10:47

Ã…ge Oterhals

I have tentatively identified this Stictis to S. f

28-03-2026 07:55

Marc Detollenaere Marc Detollenaere

Hello everybody,Yesterday I found a number of whit

26-03-2026 15:31

Ã…ke Widgren Ã…ke Widgren

Hello,I found this one in October last year, on r

27-03-2026 15:23

Gernot Friebes

Hi,this Trichopezizella deviates from typical T. b

27-03-2026 15:08

Gernot Friebes

Hi,I'm looking for help with this coelomycete on C

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Paraphyses IKI reaction in Scutellinia
Perz Piotr, 03-02-2007 23:10
Hi,

I have question about paraphyses IKI reaction in genus Scutellinia. Sometimes paraphyses stain in IKI green - it means they have carotenoids. Sometimes there is no IKI reaction - also paraphyses does not contains carotenoids.

How is the name for violet-lila reaction? Here paraphyses of Scutellinia pseudotrechispora (Schröt.) Le Gal in IKI

  • message #1406
NC NC, 04-02-2007 10:10
Re:Paraphyses IKI reaction in Scutellinia
Bonjour,
J'ai déjà observé cette réaction violette avec le Lugol par exemple avec Scutellinia crucipila. Je pense que la réaction (carotene à l'extrémité des paraphyses)en vert foncé (vert-bouteille) s'arrete par manque de concentration en iode (??) et le mélange orange + vert foncé donne cette couleur lilas. Dans ton cas une préparation sans eau, seulement avec IKI donne t-elle toujours du violet?
Amicalement
Robert
Hans-Otto Baral, 04-02-2007 11:54
Hans-Otto Baral
Re:Paraphyses IKI reaction in Scutellinia
I have actually observed the same in "Scutellinia sp." (see my paper on Lugol versus Melzer, mycotaxon 29: 428, 1987): when adding IKI (Lugol) to a water mount, the colour reaction of the paraphyses changes from greyish-violet to grey.olive or grey-brown. The violet reaction is seen at an iodine concentration of 0.05-0.1% while the olive appears at 0.1-0.2 % and higher. So the colour is concentration-dependent similar as in the hemiamyloid apical rings. But contrary to hemiamyloidity, the violet and olive are also seen when KOH-pretreated, and I believe also in Melzer.

Zotto
Perz Piotr, 11-02-2007 09:00
Re:Paraphyses IKI reaction in Scutellinia
Thanks a lot, Robert & Zotto!