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Hi! I found this interesting tiny ascomycete on i

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Sebastien BassoHello, I'm conducting a mycological inventory in
Minute bright yellow discomycete
Daniel Ghyselinck,
24-01-2012 20:29
I have collected last week this minute (max 0,4 mm) bright yellow discomycete on deciduous decorticated wood.
Paraphyses densely filled with yellow granules.
Spores about 20 – 26 x 4 – 4,5 µm, also with yellow granules, at maturity with 3 septum.
Ascus 8 spored, apex blue in IKI.
Thanks in advance for your suggestions.
Best regards,
Daniel
Hans-Otto Baral,
24-01-2012 20:46

Re : Minute bright yellow discomycete
Hi Daniel
I am very happy to see these splendid photos of living elements. This makes identification much easier and clear.
This is Calycellina ochracea, a curious species characterized by these refractive yellowish VBs in the paraphyses, in which it resembles Rodwayella citrinula.
The "granules" in the spores are oil drops. You can easily make the test: When you add KOH the spore guttules will resist but those in the Paraphyses will disappear. They are only seemingly similar.
If you have Cresyl blue you can colour the spore wall in deep lilac. The round conidia formed on the germinating spores are also typical.
The species is not frequent. I would be glad? when you can tell me the region where you collected it. No idea of the host tree?
Zotto
I am very happy to see these splendid photos of living elements. This makes identification much easier and clear.
This is Calycellina ochracea, a curious species characterized by these refractive yellowish VBs in the paraphyses, in which it resembles Rodwayella citrinula.
The "granules" in the spores are oil drops. You can easily make the test: When you add KOH the spore guttules will resist but those in the Paraphyses will disappear. They are only seemingly similar.
If you have Cresyl blue you can colour the spore wall in deep lilac. The round conidia formed on the germinating spores are also typical.
The species is not frequent. I would be glad? when you can tell me the region where you collected it. No idea of the host tree?
Zotto
Gernot Friebes,
24-01-2012 20:48
Re : Minute bright yellow discomycete
Hi Daniel,
looks similar to Calycellina ochracea.
Best wishes,
Gernot
PS.: To slow :-(
looks similar to Calycellina ochracea.
Best wishes,
Gernot
PS.: To slow :-(
Daniel Ghyselinck,
24-01-2012 20:56
Re : Minute bright yellow discomycete
Thanks a lot Zotto,
I will immediately make the test with KOH and Cresyl blue, and post the result on the forum.
It was collected in Belgium, Province Brabant Wallon, near Nivelles (Monstreux - Bois du Planti) on 15.01.2012.
Unfortunately no idea of the host, it was found by my wife (as usual, she founds everything !), in a pure deciduous mixed forest, and I just got a small piece of rather rotten wood.
Daniel
I will immediately make the test with KOH and Cresyl blue, and post the result on the forum.
It was collected in Belgium, Province Brabant Wallon, near Nivelles (Monstreux - Bois du Planti) on 15.01.2012.
Unfortunately no idea of the host, it was found by my wife (as usual, she founds everything !), in a pure deciduous mixed forest, and I just got a small piece of rather rotten wood.
Daniel
Daniel Ghyselinck,
24-01-2012 21:42