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Calycina parilis ?
Maren Kamke, 29-12-2013 23:40
Maren KamkeHi everybody,
I found this one on decidious wood. The apothecia are 0,5 mm wide, pale yellow, somewhat translucent, sessile without dark cells in the excipulum.
Hairs are difficult to find, there are two elements beside the asci. both are cylindrical. One type is 4 µm wide and filled with strongly refractive content, which does not dissappear with KOH 3%. The other one, which I think are the paraphyses are 2 µm wide and not filled. The asci are 90-102 x 10-12 µm with croziers, 8-spores, biseriate. The porus-reaction with IKL is dirty purple to blue and of calycina-type. Ascospores becoming 1-septate, rarely 2-septate and develop sidespores with age, (10-14) 11,9 x 3,7 (3-4) µm.Could it be C. parilis?
Thanks and Happy New Year
Maren
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Hans-Otto Baral, 30-12-2013 11:58
Hans-Otto Baral
Re : Calycina parilis ?
Hallo Maren

das ist "Phaeohelotium" trabinellum. Eher eine Calycina, aber auch nicht so recht.

Ich würde gerne den Fundort notieren, da ich vor 10 Jahren einen Artikel darüber angefangen haben...

Grüße
Zotto
Hans-Otto Baral, 30-12-2013 18:13
Hans-Otto Baral
Re : Calycina parilis ?
Hi Maren

I had a browser problem which unintentiously translated all english text  into German. Now I understand your description better. :-)

you thought that two kinds of paraphyses occur. No, there is only one. Those with refractive content  are alive and the narrower ones without are dead. In KOH the refractive contents disappear, certainly. maybe the KOH did not reach the centre of the squashed fungus.

Zotto
Maren Kamke, 30-12-2013 18:42
Maren Kamke
Re : Calycina parilis ?
Hi Zotto,
thank you for the information, that was enlightening.
Regards
Maren
Stip Helleman, 31-12-2013 02:20
Stip Helleman
Re : Calycina parilis ?
Hi Maren and Zotto,
Excellent timing, I had it just under the microscope and was breaking my head over the generic position. My collection differs a bit in ascuslength 73-90 x 9-11, spores 8.5-13.5 x 3.5-4 found at the bottom of a 15 cm thick decorticated Quercus branch 1,50 m high (whole tree dead)
The VBs in the low refractive paraphyses were not visible untill IKI was added.

Stip
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Hans-Otto Baral, 31-12-2013 11:59
Hans-Otto Baral
Re : Calycina parilis ?
Yes it is! I noted the folowing ascus size:
*(48-)55-93(-110) {7} x (8-)9-11(-12.5) ?m {10}

The mentioned  double (subtrabinella Bresadola) has asci
*112-150 x 11.5-12(-13.5) ?m {2}

I would also include collection data of your collection, Stip, if you don't mind.

Zotto
Stip Helleman, 31-12-2013 19:34
Stip Helleman
Re : Calycina parilis ?
Hoi Zotto,
date 29-12-13, NL, Oploo, Staatsbos. there are more collections from this area.
SBRH 789

Stip
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Hans-Otto Baral, 31-12-2013 20:38
Hans-Otto Baral
Re : Calycina parilis ?
Danke, die eine genügt :-)