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12-03-2026 19:44

Enrique Rubio Enrique Rubio

Hi to everybody.Can you give me any suggestions ab

11-03-2026 17:36

Michel Hairaud Michel Hairaud

Bonjour, Je cherche des indices  pour cette réc

12-03-2026 15:45

Åge Oterhals

Dear forum,I found this small discomycete on a ver

05-03-2026 10:07

Hulda Caroline Holte

Hello, I found and collected this species growing

08-03-2026 14:05

Thierry Blondelle Thierry Blondelle

Bonjour à tous,Sur 3 récoltes supposées de H. l

12-03-2026 16:17

Sylvie Le Goff

Bonjour à tousRécolté dans le 22 en France (ré

12-03-2026 14:37

David Wasilewski

These small ascomycota (1-3 mm) ere observed growi

11-03-2026 16:48

Bruno Coué Bruno Coué

Bonjour, je serais heureux d'avoir votre avis sur

09-02-2026 22:01

ruiz Jose

Hola, me paso esta colección en madera de pino, t

11-03-2026 14:14

Gernot Friebes

Hi,I would like to share a collection of Scopinell

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Eutypa lejoplaca?
Garcia Susana, 23-01-2014 14:32
Hi all

I found this Pyrenomycete, growing on wood of Acer campestre.
Perithecia size is 200-260um in diameter. Ostioles are 115-140um in diameter and have a cruciform shape.
Asci measured 55-68 × 5-7um, area spores 15-23 × 5-6.5um. With IKI, the apical apparatus becomes more apparent, but I'm not able to say if it is IKI +. in any case would be weakly positive.
Persistent paraphyses.

Following keys Rappaz, came to Eutypa Lejoplaca but I doubt if it could be another species, such as E. maura, or leptoplaca (if I consider the reaction to IKI is +).


Can anyone identify the species with the data provided?


Thank you,


Susana (Spain)

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Jacques Fournier, 23-01-2014 17:49
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
you are right, your fungus is likely around E. leptoplaca and E. lejoplaca. Eutypa maura can be ruled out because it is distinctive in having more immersed perithecia and the wood markedly blackened beneath the surface between the necks.
The most reliable difference between the two species is the reaction of the apical apparatus to iodine.
I suggest you follow Rappaz's procedure with a short pretreatment of perithecial content in 3% KOH and mounting in Melzer's reagent. This leads to a more drastic retraction of the ascal content than IKI and makes the apical apparatus easier to make out. Remember it is in fact subapical, at the bottom of a shallow tubular structure. The thing is to work on a very small amount of material in order to make a very thin slide, otherwise interpreting the iodine reaction is most often tricky, especially in species where the apical apparatus is very small. This applies to all species of Diatrypaceae since the character of reaction to iodine is crucial.
Come back with a clear result, whatever how long it takes!
Cheers,
Jacques
Garcia Susana, 23-01-2014 21:50
Re : Eutypa lejoplaca?
Salut Jacques

¿C'est la réaction tout aussi intense avec KOH + Melzer, qui KOH + IKI?


J'ai fait quelques préparations KOH + IKI (je n'ai pas de Melzer) et pense que c'est positif, j'observe dans les asques un couple de très petits points que je dirais sont bleus.


Que pensez-vous?


Saludos


Susana

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Jacques Fournier, 24-01-2014 08:56
Jacques Fournier
Re : Eutypa lejoplaca?
Hola Susana,
ton excellente photo montre bien une réaction positive, amyloide. Tu peux donc écarter lejoplaca.
E. leptoplaca est beaucoup plus fréquente sur Fraxinus, tu devrais la trouver facilement sur des petites branches tombées récemment avec le vent et tu pourras ainsi lui comparer ta récolte.
Amitiés,
Jacques
Garcia Susana, 24-01-2014 11:04
Re : Eutypa lejoplaca?
Muchas gracias Jacques

Saludos.
Susana