28-04-2024 18:05
Bernard CLESSE
Bonsoir à toutes et tous,J'ai trouvé ce matin ce
24-03-2024 08:27
Thierry Blondelle
HiOn Hedera helix fallen branchEcological habitat:
26-04-2024 10:07
Mathias Hass
Hello, Does anyone know what this is? Found on J
24-04-2024 21:54
éric ROMERO
Bonjour, J'ai trouvé ce Lasiobolus sur laissées
23-04-2024 15:18
Lothar Krieglsteiner
... but likely a basidiomycete. I hope it is o.k.
23-04-2024 13:17
Edouard Evangelisti
Bonjour à tous, Je viens de récolter ce que je
23-04-2024 21:49
Ethan CrensonHello all, A friend recently found this orange as
22-04-2024 11:52
Zuzana Sochorová (Egertová)
Hello,I made a loan of a collection of Microstoma
11-01-2022 16:36
Jason Karakehian
Hi does anyone have a digital copy of Raitviir A (
Hello everyone,
I hope anyone of you can give me a hand with this problem
On a pine cone I found a pair of yellow / orange slices. I'm not quite sure but think that there are only fours pores in the asci. The measure of the asci is between 75-85 x18 µm. The measure of the spores is between 23-26 x 4-7 µm. I thought there were konidiensporen but were appendages of the spores. Parafysen are knobby, J-.
Is there anyone who has an idea in which direction I should think?
Greetings
Gannie
If the asci are IKI+, it might be Pezicula eucrita. It grows right now, after the rains in mid-europe.
Cheers,
Guy
Hello Guy,
Thank you, I looked for some pictures and I think you're right. Can you explain what IKI+ and how to see this?
Greetings
Hannie
IKI is the so called Lugol´s solution (or "J" - but without chloral hydrate).
With such IKI, your fungus will show a hemiamyloid (= red!) reaction of the AA (apical apparatus).
In Melzer´s reagent, the reaction is negative.
Pre-treated with KOH (5%), the reaction will be blue in Melzer´s and in Lugol´s solution and not turning into red after a little while..
Cheers,
Guy
Hello Guy,
thanks for explanation. I did it with Lugol but I can't see any reaction.
Greetings
Hannie
Hello Guy,
yes it's pale yellow. It's hard to get it here in Holland, so someone sent it to me. Maybe he put some water to it? So it's better to buy some new?
Hannie
to my experience there are several varieties of Lugol's solution in trade. I once got one that was almost clear and prepared for lichenologists, since the reaction in lichen is very strong, but gives no sufficiant results with "standard" ascomycetes. On the other hand I experienced false descriptions of the reaction where concentrations of iodine stronger than 2% were used and thus an hemiamyloid reaction was suppressed or overrun.
The standard formula for Lugol's for ascomycetes should be 1% iodine + 3% potassium iodide added to water.
Regards
Martin
I have crystalline iodine and potassium iodide and mix it needed.
See http://www.invivoveritas.de/articles/iodine-reaction/
Zotto
Martin and Zotto,
thanks for the answer. I'll study your paper Zotto but it will take some time for me.
Cheers
Hannie