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Spores of Leotia lubrica are septate?
    
                    Masanori Kutsuna,
                17-09-2014 03:33
    
    According to some literatures (Dennis, Breitenbach and Kranzlin ...),
ascospores of Leotia lubrica have 3 or more septa at maturity.
But I can't see any septa in Japanese materials.
What should I do to see septated spores?
Does anyone have microphotos of septated spores?
Masanori Kutsuna
                                    Hans-Otto Baral,
                                17-09-2014 08:55            
            
                Re : Spores of Leotia lubrica are septate?
                Leotia and Microglossum do not develop septa at all. I never saw them. A frequent mistake with herbarium material is that plasma bridges between the big oil drops are taken for septa (they are called "pseudosepta"). But with some experience or when staining with Congo Red I think they can safely be distinguished from true septa. With living spores there is no possibility to recognize any septation. The spores do not germinate easily, so it is possible that under optimum conditions the spores might indeed get septate during germination.
I remember a drawing by Chadefaud who illustrated eguttulate, clearly septate spores for Leotia. I never understood it, maybe it was a misidentification. Te drawing in Breitenbach & Kränzlin looks indeed convincing, with both septa and big oil drops. But it was made in the dead state, and I am sceptical about it.
Zotto
                
                
                
                
                
                            I remember a drawing by Chadefaud who illustrated eguttulate, clearly septate spores for Leotia. I never understood it, maybe it was a misidentification. Te drawing in Breitenbach & Kränzlin looks indeed convincing, with both septa and big oil drops. But it was made in the dead state, and I am sceptical about it.
Zotto
                                    Masanori Kutsuna,
                                17-09-2014 09:24            
            Re : Spores of Leotia lubrica are septate?
                Thank you very much for your detailed explanations, Zotto.
Kutsuna
                
                
                
                
                
                            Kutsuna