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Cercophora on wood
Dartanha Soares, 29-04-2015 16:49
Dartanha SoaresHi to all,

Found this fungus on very rotten twig (unknown). I believe it is a Cercophora species, but I was not able to found a suitable species, because I do not have the necessary literature.
The ectal excipulum called my attention because it is constantly ruptured forming a peculiar pattern.


Perithecia up to 500 microns. Ascospores: upper dark cell 18-20 x 11-12.5, hyaline basal cell 30-45 x 4-6 microns. Asci (young?) up to 200 x 13-18 microns.


Figure Bars: A-B = 1 mm; C = 0.5 mm; D = 100 micra; E-G = 50 micra; H-P = 25 micra


Could somebody help me in this identification.


Thank you very much.


Dartanha


 

  • message #35473
Dartanha Soares, 02-05-2015 02:49
Dartanha Soares
Re : Cercophora on wood
Hi,
Anyone have a suggestion?
Thanks!
Peter Püwert, 02-05-2015 18:45
Peter Püwert
Re : Cercophora on wood
Hi Dartanha,
please compare with this species. I found it in rotting Trametes and in Acer wood.
Greetings
Peter.
  • message #35543
Michel Delpont, 02-05-2015 19:12
Michel Delpont
Re : Cercophora on wood
I agree with Peter, but could you check the length of gelatinous appendages and the presence and type of hair.

Michel.
Dartanha Soares, 04-05-2015 16:43
Dartanha Soares
Re : Cercophora on wood
Dear Peter and Michel,
Thank you for your comments.

I have only the Ellis & Ellis description of C. caudata, and I think the description doesn't match, mainly because of the lack of hair in the perithecia neck (however, I dont know if this is a strong characteristic) and also because the ascospores are larger. In fact, based on Ellis & Ellis, the ascospore size (apical dark cell) and filamentous appendage of this specimens seems to fit with C. coprophila (?????).


The filamentous appendages of the young, sigmoid, ascospores are most 20-35, but reached up to 50 microns long (see figures H and I, on the plate). I was unable to obtain the size of such appendage from the mature ascospores because in most cases they vanished.


In my new attempts I did not get a good section of the perithecium, but seems to me that it is glabrous, or with flexuous brown hairs (half bottom) (see figure E on the plate). After 12 hours on dew chamber a white tomentum was present (figure attached here).


So, what do you think?


Thanks again,


Dartanha

  • message #35581
Michel Delpont, 04-05-2015 19:25
Michel Delpont
Re : Cercophora on wood
The hair is of course a major character; you speak of "globulus" but normally this applies to globulus who is or is not at the top of the ascus apical under the appliance. On your photos can not be distinguished whether it is present or not. Is it smooth or decorated? It is also an important character.
C.coprophila only grows on dung.

Michel.
Peter Püwert, 04-05-2015 21:48
Peter Püwert
Re : Cercophora on wood
Hi all,
one cannot assign the kind only with E. et E. unambiguously. There is literature which makes simpler: HILBER, R. & O. HILBER (1979): Einige Anmerkungen zu der Gattung Cercophora Fuckel (Lasiosphaeriaceae).  Zeitschrift für Mykologie 45(2): 209-233.   I have this as a PDF, but in German.

With Your image it is no tomentum, but probably a hyphotrama. The tomentum would have to look in such a way, like to the added photos from Cercophora coprophila.

Greetings
Peter.
  • message #35587
  • message #35587
  • message #35587
  • message #35587
Dartanha Soares, 04-05-2015 22:49
Dartanha Soares
Re : Cercophora on wood
Hi,
Sorry for the misuse of "globulus". I made an update in the previous post to remove that term, because, in fact, I didn't saw it.
I tried to do new slides to look for the presence of the globulus in the tip of the ascus. I used only water and MLZ, but as before I didn't saw it. Is its presence/absence an important characteristic? I need to use a specific mounting medium?
Definitely the tomentum of C. coprophila is very different from what I saw.
During the last weekend, after read some posts here in the forum about Cercophora, I found the Hilber & Hilber pdf file, but unfortunately my german is null! So, it is probable that this specimen remains as "Cercophora sp.", unless you have another suggestion.
Well, thanks again for your comments, I really appreciated.
Dartanha
Michel Delpont, 05-05-2015 09:45
Michel Delpont
Re : Cercophora on wood
The presence or absence of blood cell on top of the ascus is an important character. You can also try to observe it in the blue cotton. also the hair, shape and distribution and the structure of the wall of perithecium are important.
I have no translation of the work Hilber, but I send you another paper directly ( Lundqvist ), hoping that it might help you.
Regards.
Michel.
Dartanha Soares, 05-05-2015 13:15
Dartanha Soares
Re : Cercophora on wood
Hi Michel,

Thanks a lot for the Lundqvist's paper, I pretty sure it will be useful
Merci beaucoup! :@)
Peter Püwert, 05-05-2015 22:43
Peter Püwert
Re : Cercophora on wood
I observe a lot with CR, watery solution. This is not so aggressively.
Michel, have You the Lundquist- paper for me ?
Thanks in advance.
Peter.
  • message #35619
Dartanha Soares, 06-05-2015 13:09
Dartanha Soares
Re : Cercophora on wood
Hi all,

In Lundqvist's description of C. areolata (pag.105) he mentions: "Pedirium ... areolate, cracking in 50-100 u broad polygones when crushed;"
In his comments he says that this trait is present in very few species of the genus, and all species listed, but C. striata, are coprophilous.
I found a full description of C. striata in "Miller, A. N. & S. M. Huhndorf (2001). Neotropical Ascomycetes 10. New and interesting Cercophora species. - Sydowia 53(2): 211-226." and I think that my specimen is close related with C. striata.


However, I didn't saw the presence of the "white to grayish granules or crystalline flakes below the neck" as illustrated in figures 41 - 43 of Miller & Huhndorf work, and the asci are shorter and wider (up to 260 long x 11-18 wide - see figure attached) when compared with C. striata that has "asci (278-) 305-384 (-413) x 4-9.5".


An additional information not mentioned before is that the sample was collected in Southeast Brazil. I also made an additional slide using cotton blue and the subapical globulus was absent.
So, the absence of the globulus plus the areolate peridium surface, that cracks when crushed, and its habit fits with C. striata. What do you think?
Thanks again,
Dartanha

  • message #35634
  • message #35634
Michel Delpont, 06-05-2015 14:34
Michel Delpont
Re : Cercophora on wood
Hello Dartanha

I do not know the article you speak (if you can send it to me, thank you), but it seems that your fungus is still different from C.striata. I read that some Cercophora of America (C.appalachianensis and C.californica) have cells that have Munk pores. Would you have seen this character on your mushroom? This could be a track for you, at least Andrew is an idea.

Michel.
Dartanha Soares, 06-05-2015 15:35
Dartanha Soares
Re : Cercophora on wood
Hi Michel,

I sent the article to your email.

No, I didn't saw the Munk pores, I had some difficulties to obtain a good section of the peridium wall. I need to try again.

Dartanha