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Cyathicula amenti
Nina Filippova, 30-04-2015 20:42
Good evening,

these very rich apothecia were found on fruit catkins of Salix sp. on wet soil after snow melt. I would appreciate your help with prior identification.


Apothecia stipitate, white to translucent, hymenium and outer surface the same color, up to 0.5 mm high, 0.7 mm broad (disc) and stem 0.2-0.3 mm thick, outer surface shortly pubescent, edge without pronounced hairs; crowded on last year fallen fruit catkins of Salix sp.


Excipulum from textura porrecta, the outer layer mady by cylindrical obtuse thin-walled hyphae filled with refractive valuoles, inner layer of prismatic cells with thickened walls; asci cylindrical, bent, with crozier, with euamyloid pore, 100-120 x 9-12 in vital state; paraphyses cylindrical, evenly enlarged to upper part, where 4 broad, with abundant small VBs in upper part, reacting reddish-brown with IKI; spores subfusoid, heteropolar, slightly bent, 15 (12-15.7) x 4.7 (4-5.4).


Nina.

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Lothar Krieglsteiner, 30-04-2015 20:55
Lothar Krieglsteiner
Re : Calycellina, probably

Hi Nina,


why not Calycina amenti? (Pezizella amenti, Cyathicula amenti).


 Best regards from Lothar

Stip Helleman, 30-04-2015 20:57
Stip Helleman
Re : Calycellina, probably
Hi Nina,
this one looks like Crocicreas amenti (Batch) Carpenter, now I dont have a good generic position present.
cheers,
Stip
Hans-Otto Baral, 30-04-2015 20:57
Hans-Otto Baral
Re : Calycellina, probably
Hi Nina

I do not know why you have such large asci and long spores, but this can only be Cyathicula amenti (formerly Pezizella amenti). Typical spore size is about 9-11 x 3.5-4 µm.

Zotto
Nina Filippova, 30-04-2015 21:37
Re : Calycellina, probably
Thank you to all (very fast and unanimous ID!),

Zotto, i have measured additionally another two apothecia and spore size decreased, but not very much (and my microscope calibration checked recently):

13.7 (11.5-15.7) x 4.6 (4-5.4) (n=36).

Nina.
Hans-Otto Baral, 30-04-2015 21:47
Hans-Otto Baral
Re : Cyathicula amenti
very strange indeed! Even Boudier found only 8-10 x 3.5-4

Forgot to say that the apical ring is of the Hymenoscyphus type.
Nina Filippova, 04-05-2015 18:50
Re : Cyathicula amenti
Good evening,

today i have collected this species from another location 1 km apart from the first, and checked the spores again. They are that large too:

Spores 15 (13.5-16.4) x 4.6 (4.2-5), n=20.

That seems that our population of the species has larger spores. Other features are very similar to shown in Zotto's collection.

Here the picture of spores (bar=10 mk).
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Hans-Otto Baral, 04-05-2015 19:22
Hans-Otto Baral
Re : Cyathicula amenti
Indeed very unexpected! The highest length I find in the literature is 12 µm.