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Calycellina leucella?
Raúl Tena Lahoz, 30-01-2008 18:49
Raúl Tena LahozHi to all!
I need a bit of help...

Collected in Valencia (Spain), 1 meter above sea level, on 26-12-07.
Apothecia gregarious, sessile (or subsessile), on both sides of fallen leaves of Ceratonia siliqua. Reaching 0,7 mm. More than 150 apoth. together. Whitish cream to pale ochraceous when old.
Asci: 8-spored, with croziers, MLZ + (Water + Melzer).
Spores: hyaline, ellipsoid to fusoid, often with strange forms, tiny polar guttules when alive, 2 polar (up to 1,5 µm) guttules when dead? (9)12-17 (20) x 2,5-3,5 (4). Only a few two celled.
Paraphyses: x 2-3,5, with yellowish pigment.
Ectal excipulum of textura angularis to prismatica. Brown basal ring present. “Hairs” apically rounded, 4-septate?,with same pigment as in paraphyses.
Can it be Calycellina leucella (Karst.) Dennis ex Müller?
Thanks a lot for your help!

Raúl Tena Lahoz.
Hans-Otto Baral, 30-01-2008 22:20
Hans-Otto Baral
Re:Calycellina leucella?
Very good photos in vital state! The yellowish pigment are refractive vacuoles typical of Calycellina. The brown round cellular structure is the scutum, a protection of the very young apothecium that can still be found at the base of the adult apo.

I don't think for leucella which has another spore shape (median constricted, apically often slightly curved - scutuloid), see below.
  • message #3955
Hans-Otto Baral, 30-01-2008 22:20
Hans-Otto Baral
Re:Calycellina leucella?
free spores
  • message #3956
Hans-Otto Baral, 30-01-2008 22:21
Hans-Otto Baral
Re:Calycellina leucella?
Rather I think it is C. populina although I have usually noted slightly short spores here, f.x. *(8.5-)10-13(-15) x 2.6-3.2 µm (photo below has a smaller scale thanh those above).

Important is that the hairs are cylindrical and not tapered like Hyaloscypha. In that case one should seek in Phialina in Huhtinen's monograph.

Zotto
  • message #3957
Raúl Tena Lahoz, 31-01-2008 21:16
Raúl Tena Lahoz
Re:Calycellina leucella?
Thanks a lot Zotto but my photos are no so good as your drawings!
I feel it´s more close to Cll. leucella than to populina but I have no experience with these species. Some spores are slightly scutuloid I think, and some have capitate head (if spores with capitate head are like those of the bottom of your second photo on the left). Also a few had median constriction but not so much spores as seen in yours photos (on DVD). And the length of spores is nearly always over 12 when mature. Under 12 are rare.
Sorry, I forgot to indicate that the poils are cylindrical. The fifth photo in my doc is of the poils at the margin. The fourth photo is of the poils at the middle of the apothecium.
Still Cll. populina?

Thanks again for your help!
Raúl.
Hans-Otto Baral, 04-02-2008 00:12
Hans-Otto Baral
Re:Calycellina leucella?
Sorry for late response. I have not enough images of C. leucella to show me and you how that species looks. I think it is macroscopically different, not such white, without convex and pruinose disc. On the other hand, I have the impression that there exist more than one species with such leucella characters, considering the variation in spores and excipulum. Perhaps typical leucella has a distinctly gelatinized excipulum up to the margin (Calycellina leucella, HB 6225b.JPG) while other finds have gel only in the basal excipulum (Calycellina leucella, HB 6454.JPG). My C. populina finds are non-gelatinized all over.

Zotto
Raúl Tena Lahoz, 04-02-2008 14:41
Raúl Tena Lahoz
Re:Calycellina leucella?
Ok, no problem. I’m still novice studying the excipulum. For me it’s hard to distinguish when it’s gelatinized or not. I will try to find more apothecia in the future and look after for it. I can only add these bad photos. Maybe it had gel in the basal excipulum but I think it hadn’t on the margin. No sure of nothing as you see...
Thanks a lot for spending your time with me.

Raúl.
Hans-Otto Baral, 04-02-2008 16:09
Hans-Otto Baral
Re:Calycellina leucella?
Your photos are splendid! The problem with gel in the excipulum is that the gel layer gets thicker (and the cell lumina narrower) but less refractive when you study dead material. So a living ectal excipulum may look quite thin-walled, but if you add lactophenol odr MLZ or KOH you see much thicker common walls between the cells. So I recommended to study both live and dead in such a case.

Zotto
Raúl Tena Lahoz, 04-02-2008 18:57
Raúl Tena Lahoz
Re:Calycellina leucella?
Ok, I will try it next time. Always learning.
Thanks a lot!

Raúl.
Jean-Louis CHEYPE, 06-02-2008 13:23
Jean-Louis CHEYPE
Re:Calycellina leucella?
Hello,
Here are some pictures of what I believe to be Calycellina leucella, collected in alpine area at the Col du Joly (2000 m) 14/09/2007, on cone of Alnus viridis. See ascospores on 2 °page
Hans-Otto Baral, 06-02-2008 16:16
Hans-Otto Baral
Re:Calycellina leucella?
Hi Jean-Louis

Yes, this should be leucella. The large spores obviously exclude Calycellina alniella. The spores partly have the constiction (exactly where the nucleus is), therefore the spores look like septate but there isn't any septum. Did you study the excipulum? Any hairs?

Zotto
Jean-Louis CHEYPE, 06-02-2008 18:06
Jean-Louis CHEYPE
Re:Calycellina leucella?
Hi Zotto,
Thank you for your answer, I have no other micro photo. My collection fits your HB6620E + HB6454E.
Best regard
Jean-Louis :o)
Raúl Tena Lahoz, 07-02-2008 10:10
Raúl Tena Lahoz
Re:Calycellina leucella?
Yes, Calycellina leucella is not my species. Macro seems diferent, also I haven´t seen so much constrictions in spores and such measures. Thanks a lot Jean-Louis for your good photos.

Raúl.