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Violet-brown disco on burnt ground
Yatsiuk Iryna, 14-05-2016 10:58
Yatsiuk IrynaHello, all,
I have no idea about it.
Found in spring, on burnt ground. Fruitbodies up to 0,8 cm in diam, grey when dry, violet-brown when rehydrated, with white crusty margin. Fruitboies were dry when collected.
In IKI exuding strong violet pigment.
Numerous anchoring hyphae are present at the base.
Asci 8-spored, 10-13 um wide, length I cannot measure properly, not blueing in iodine.
Paraphyses branching, containing brown pigment, 3-5 um wide. 
Spores smooth, sometimes containing one strongly refractive central guttule, 10-13.5*6.3-8.0 um.
What do you think?
Iryna
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Yatsiuk Iryna, 14-05-2016 11:00
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
Excipulum
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Yatsiuk Iryna, 14-05-2016 11:04
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
Ascus, paraphyses. Cross-section (in IKI, at first it turns violet, than red-brown)
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Hans-Otto Baral, 14-05-2016 11:05
Hans-Otto Baral
Re : Violet-brown disco on burnt ground
Hi Irina
do you mean extruding violet pigment in KOH or in IKI?

I am quite sure it is an operculate species.

The drops in the spores are not lipidic but gaseous (DBBs).

Zotto
Yatsiuk Iryna, 14-05-2016 11:14
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
Dear Zotto,
I mean in IKI. I noticed it when in checked asci for IKI-reaction. I haven't studied it in KOH. In water it didn't show this strong violet colour.
Thank you for notice about drops, is it not valuable feature than?
Best regards,
Iryna
Nicolas VAN VOOREN, 14-05-2016 11:17
Nicolas VAN VOOREN
Re : Violet-brown disco on burnt ground
Maybe a Pseudombrophila species...
Yatsiuk Iryna, 14-05-2016 11:18
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
I thought about Greletia species first, but paraphyses seems not fit.
Best regards,
Iryna
Yatsiuk Iryna, 14-05-2016 11:22
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
Thank you for the idea, Nicolas, I'll check keys for Pseudombrophila. 

Iryna
Hans-Otto Baral, 14-05-2016 11:52
Hans-Otto Baral
Re : Violet-brown disco on burnt ground
The presence of DBBs strongly depend upon the method. You never see them in fresh samples because they are absent there. Obviously you rehydrated herbarium material.
Also when you add Melzer or cottonblue/lactic acid to a fresh sample you will get DBBs (some kind of vacuum) because water is extracted from the spores.
Yatsiuk Iryna, 16-05-2016 11:45
Yatsiuk Iryna
Re : Violet-brown disco on burnt ground
Thank you, Zotto,
Fruitbodies were collected already in the dead state.
Best regards,
Iryna