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12-06-2026 14:50

François Freléchoux François Freléchoux

Bonjour, Voici la brève description d'une Mollis

10-06-2026 21:16

François Freléchoux François Freléchoux

Bonsoir,Le dernier du jour, en attendant votre avi

11-06-2026 16:24

Ethan Crenson

Hi all, Recently, a friend found this Orbilia in

11-06-2026 19:01

William Slosse William Slosse

Hello all,In an attempt to make a culture of a sus

11-06-2026 19:03

Nicolas VAN VOOREN Nicolas VAN VOOREN

Chers membres d'Ascofrance,Le site sera placé en

10-06-2026 23:08

éric ROMERO éric ROMERO

Bonjour tous, Je vous propose un Mollisia trouvé

09-06-2026 18:32

Camille Mertens

Sur morceau de roseau immergé 0,5 - 0,7 mm de dia

10-06-2026 12:54

Steve Clements

Bonjour encore, Pouvez-vous m'aider, s'il vous pl

10-06-2026 21:07

François Freléchoux François Freléchoux

Toutes les tiges de gentianes jaunes de l'an passÃ

10-06-2026 13:41

François Freléchoux François Freléchoux

Bonjour à nouveau, Voici une trouvaille d'hier.

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Ascomata under epidermis Carex
François Bartholomeeusen, 25-05-2026 16:44
Hi forum members,

During an excursion organised by Antwerpse Mycologische Kring on 9 May 2026 in the Abtsheide nature reserve in Beerse, Belgium, I found black, round ascocarps beneath the epidermis of Carex leaves. The average diameter is 73 to 140 µm, with a central ostiole smaller than 30 µm and without a papilla.
Exipulum: black with a texture varying from prismatica/angularis to intricata
Asci: bitunicate, thick-walled especially at the apex (with a short papilla?); clavate (55–116 × 10–13 µm); containing 8 spores; biseriate to irregular arrangement; reaction to Lugol's solution: dextrinoid;
Spore: subcylindrical to fusiform (sometimes slightly curved), with 1 central septum, slightly constricted at the septum and 2 large and several small droplets in each half; 29–35 × 6–7 µm
Could it be Metasphaeria cumana var. macrospora?
Any help would be most welcome!

Kind regards,
François Bartholomeeusen

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Eduard Osieck, 26-05-2026 20:36
Re : Ascomata under epidermis Carex
Unfortunately I have never found this species. The spore size is much smaller than given in the protologue: 36-42 x 6-8 um. I am not aware of a modern description. You could compare with other species with this type of spores such as Massarina lacustris (Leuchtmann 1984), Massarina amphibia (Magnes & Hafellner 1991, see also Aptroot 1998), Lentithecium (Massarina) fluviatilis (Aptroot & Van Ryckegem 2001), and Stagonospora perfecta (Quaedvlieg et al. 2013). The latter also from Carex. Did you note a mucoid sheath around the spores (present in M. amphibia at least, best seen in Indian ink). Best regards, Eduard
François Bartholomeeusen, 27-05-2026 17:59
Re : Ascomata under epidermis Carex
Good evening, Eduard,

Thank you very much for your interesting comments!
I have reviewed all the photos showing spores and (see first photo) found two spores that appear to have a mucilaginous coating. Today I tried to examine the carex leaf again, but was unsuccessful.
The original examination was already difficult. The first two preparations showed asci but no spores (see second photo, spore disintegrated in asci)
Then four attempts that did yield results, but also several preparations that yielded asexual forms. It's all getting a bit too complex and I'm finding it rather difficult to manipulate those super-tiny ascomata!

Best regards and thanks again,
François
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