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29-03-2024 09:21

Michel Hairaud Michel Hairaud

Bonjour, Je recherche la description du genre Mac

26-03-2024 17:14

Juuso Äikäs

These little black ascos were growing on a dead, d

28-07-2011 18:31

Alex Akulov Alex Akulov

Dear FriendsToday I made the pdf file of Velenovsk

27-03-2024 22:53

Charles Aron Charles Aron

Hi All,Recently I came across these minute, setose

25-03-2024 13:41

B Shelbourne B Shelbourne

• Hyaloscyphaceae (no VBs), Hyaloscypha: Macro a

25-03-2024 21:27

Riet van Oosten Riet van Oosten

Hello, Found by Laurens van der Linde, March 2024

26-03-2024 14:37

DAVID MARCOS DAVID MARCOS

Spores (telles qu'elles apparaissent à l'intérie

24-03-2024 08:27

Thierry Blondelle Thierry Blondelle

HiOn Hedera helix fallen branchEcological habitat:

26-03-2024 11:06

michel bertrand

Bonjour, Malgré de nombreuses recherches, je n'a

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Ursula Kozik

Bonjour et bonne journée, On m'a envoyé une tro

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Hymenoscyphus on fern rhizome
Edvin Johannesen, 20-09-2017 11:27
Edvin JohannesenThis Hymenoscyphus (?) was found on Gymnocarpium dryopteris rhizomes in Central Norway.  The spores measure approx. 14-16 (-20) x 5 microns and are elllipsoid to slightly inequilateral/navicular and often somewhat pointed at one end. A substantial portion of the spores are germinating with a hypha arising from one end of the spore.  Spores are becoming one-septate at maturity.  Excipulum consists of a pseudoparenchyma - large spherical/semiprismatic cells up to 30 microns.

I don't find any Hymenoscyphus with this combination of host/substrate and spore measures.  Can anyone help?  Am I in the wrong genus?
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Hans-Otto Baral, 20-09-2017 12:00
Hans-Otto Baral
Re : Hymenoscyphus on fern rhizome
Hmm, this seems to be a very interesting collection, but it would require a lot of further data to find out what it could be. How large are the apos?

The species could belong in the relationship of Cudoniella, but to verify this I should see the living paraphyses (guttulate?). Did you test the ascus apex with iodine? The shape of the amyloid ring would be important.

typical Hymenoscyphus species have prismatic cells or even t. porrecta.

I am unaware of such a species on fern rhizomes.

Zotto
Edvin Johannesen, 20-09-2017 12:40
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
The apos are 4-9 mm in diameter, short-stalked.  I am not able to see any ascus apex/pore staining with iodine (Meltzer's) (I tested the Meltzer on a Peziza yesterday).

Ascus pore:
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Edvin Johannesen, 20-09-2017 12:41
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Paraphyses:
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Edvin Johannesen, 20-09-2017 12:41
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Germinating spore:
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Edvin Johannesen, 20-09-2017 12:42
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Excipulum:
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Hans-Otto Baral, 20-09-2017 13:13
Hans-Otto Baral
Re : Hymenoscyphus on fern rhizome
I am not sure if you mounted the fresh specimen and in what medium. The elements are all dead, which makes it very complicated.

With that size I would consider Cudoniella tenuispora but the large size and the angular excipulum rule out that option.

To show the variation of spore size would also be helpful, and please in water in order to see the contents.

Sorry for being so demanding.
Edvin Johannesen, 20-09-2017 13:32
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Sorry. I will try to mount in water.  More to come.
Edvin Johannesen, 20-09-2017 14:04
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Mounted in water, 1000x, Phase contrast.

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Edvin Johannesen, 20-09-2017 14:05
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
And more:
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Edvin Johannesen, 20-09-2017 14:06
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
And finally (sorry if the quality is still bad):
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Edvin Johannesen, 20-09-2017 14:07
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
Images of fresh specimens from the collector.  The mounts were made from semi-dried specimens, still soft.
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Hans-Otto Baral, 20-09-2017 17:10
Hans-Otto Baral
Re : Hymenoscyphus on fern rhizome
Thanks, yes, these are living spores and they contain a few small oil drops at the ends. The phase contrast that you use is difficult for me to interpret. For the spores it seems o.k., but difficult to tell the refraction index (strongly or weakly refractive).

In the paraphyses I see some large round drops but I am not sure are they refractive (I assumne so) or not. Under bright field this would have been clear.

Spore size in C. tenuispora is 12-16 x 4-5.5 µm, so I think this would fit. The septum is very probably a matter of the overmature spores.

The only thing that makes me wonder is the angular texture. That of C. tenuispora is an intricate prismatica, and the cortical cells also contain refractive drops and form hairs, here in surface view.
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Edvin Johannesen, 21-09-2017 12:12
Edvin Johannesen
Re : Hymenoscyphus on fern rhizome
OK, thanks. I shall have to check the cortical cells.