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15-02-2019 20:44

Edvin Johannesen Edvin Johannesen

Hi! These black, semi-immersed/erumpent apothecio

15-02-2019 20:32

BERNARD CLESSE BERNARD CLESSE

Pouvez-vous m'aider à identifier ce Rutstroemia (

11-02-2019 12:32

Stephen Mifsud Stephen Mifsud

Can this be identified from the macro-photos alone

15-02-2019 11:03

Edvin Johannesen Edvin Johannesen

Hello! Can anyone tell me how to distinguish Nect

12-02-2019 15:49

Martzuku Eleni

In decaying leaves of castanea and olive tree and

14-02-2019 22:15

Salvador Tello

Hola.Este hongo lo recogí en agosto del 2018 en S

14-02-2019 22:32

Ethan Crenson

I found these minute black apothecioid ascos on ba

14-02-2019 21:00

Patrice TANCHAUD

Bonsoir, sur cône d'épicéa. Apothécie 250 µm

11-02-2019 15:16

Blasco Rafael Blasco Rafael

Hola, esta especie al verla pense en Eutypella sco

11-02-2019 14:56

Savic Dragisa

Very small (-0.2mm diam), on the bark of Fagus syl

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Pezicula or Neofabraea on Malus
Gernot Friebes, 16-05-2018 15:15
Hi,

this fungus was collected by a friend on a dead, corticated branch of Malus. At first I didn't even think that it might be a Pezicula because of the conspicuously dark outside. According to Verkley (1999) there are five taxa on Malus: P. corticola, P. sepium, N. alba, N. malicorticis and M. perennans. 

P. corticola apparently comes close but I'm not sure if it can have such a dark outside. Other than that, it seems to fit well.

P. sepium can probably be excluded due to its ascospores having a smaller L/W ratio.

N. alba and N. malicorticis can be excluded due to having different conidia.

N. perennans probably comes closest. Verkley mentions (under N. malicorticis) a darker tissue that lines the discs, maybe that corresponds to our material? Difficult to say without images to compare...

Overall, I think that this collection is either P. corticola or N. perennans, more likely the latter. Does anyone have any experience with this group?

The ascospores measure 19.5-22.5 x 6-8 µm. The images attached to this post show the sexual state. I will show images of the anamorph in the next post.

Best wishes,
Gernot
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Gernot Friebes, 16-05-2018 15:19
Re : Pezicula or Neofabraea on Malus
Here is some information on the anamorph. The conidia measure up to 23 x 8 µm. I assume that the partly germinating, slightly brownish and transversally septate spores are also conidia but I'm not sure. The apothecia grow at least partly on conidiomata as can be seen on the section.

Thank you!
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Hans-Otto Baral, 16-05-2018 15:51
Hans-Otto Baral
Re : Pezicula or Neofabraea on Malus
Hi Gernot

I think you can exclude Pezicula as longa s Neofabraea is accepted as a separate genus. Indeed the whitish hymenium and dark brown exterior are typical.

This genus seems to be tricky, I have no clue to distinguish these species. Maybe you know by drawing of N. cf. alba (HB 4025) in my Neofabraea folder. the conidia there are much larger than yours, but the ascospores are similar.

There exist sequences of different species, so this could be an approach to come nearer to the truth....

Könntest du mir bitte deine Fotos per Mail schicken, dann spare ich mir das mühsame Herunterladen?

Zotto
Gernot Friebes, 16-05-2018 17:33
Re : Pezicula or Neofabraea on Malus
Hi, Zotto,

thanks a lot for your response. I am already pretty happy to know that this is a Neofabraea rather than a Pezicula.


What still confuses me is that the authors of the following paper write "The apothecia of Neofabraea and Pezicula are similar, but excipular tissues are less differentiated in Neofabraea", since I would estimate that the excipulum of our fungus is rather well-defined, and it looks like this is the case in your N. cf. alba too.


http://www.westerdijkinstitute.nl/images/ResearchGroups/Phytopathology/pdf/2016_Chen_Neofabraea.pdf.>

Ich schicke dir die Fotos gerne, blöderweise hab ich erst kommende Woche wieder Zugriff darauf.


Schöne Grüße
Gernot

Gernot Friebes, 16-05-2018 19:55
Re : Pezicula or Neofabraea on Malus
Hello, Adam,

thanks for sharing! Our fungus was also collected together with Tympanis alnea, for what it's worth. It would be great if you found conidia in your sample but I do wonder if they are generally still present in senescent material.

Best wishes,
Gernot
Hans-Otto Baral, 16-05-2018 21:49
Hans-Otto Baral
Re : Pezicula or Neofabraea on Malus
Adam: the crystals are interesting, but I cannot see them on your pics. The apical ring reaction was after KOH?

Gernot: your link does not work on my computer (server error).
Gernot Friebes, 16-05-2018 21:51
Hans-Otto Baral, 16-05-2018 22:08
Hans-Otto Baral
Re : Pezicula or Neofabraea on Malus
Oh thanks, I did not know this article. Interesting what they all erected. But the word hemiamyloid you will search in vain there. The method is ackward, e.g. neocinnamomea is described as "MLZ + with KOH-pretreatment". No mention of the red reaction.....
Adam Polhorský, 19-05-2018 13:53
Adam Polhorský
Re : Pezicula or Neofabraea on Malus
The what I think are crystals can be seen here https://drive.google.com/open?id=1zLHvFw_kRzGZ7TfW9QD8HKypmLo9yxrZ or in the apo section when zoomed in.

Yes, the blue reaction is in KOH+IKI, red in IKI.

Gernot, as you thought no anamorph left for me to observe... Many trees in the orchard were attacked by this fungus, should not be a problem to recollect them in better state.