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07-12-2025 16:07

Arnold Büschlen

Hallo, ich habe in einer Moos-Aufsammlung (epiphy

08-12-2025 21:04

Mark Stevens

"Hello everyone,I'm relatively new to microscopy (

09-12-2025 12:06

Andgelo Mombert Andgelo Mombert

Bonjour,Je recherche l'article concernant Hypobryo

08-12-2025 18:59

Lothar Krieglsteiner Lothar Krieglsteiner

.. found by a seminar-participant, I do not know t

08-12-2025 21:18

Buckwheat Pete

Hello everyone, Is it possible to at least approx

07-12-2025 17:43

Malcolm  Greaves Malcolm Greaves

This Helvella was in mixed woodland. Uniform cupul

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

16-03-2014 22:00

Ralph Vandiest Ralph Vandiest

Hello,I found this species a few months ago but ha

08-12-2025 13:39

Thomas Læssøe

https://svampe.databasen.org/observations/10572899

07-12-2025 22:43

Andreas Millinger Andreas Millinger

Good evening, tried to determine with Munks Valsa

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul