Accès membres

Mot de passe perdu? S'inscrire

24-12-2025 18:12

Ethan Crenson

Hello all, A friend found this Orbilia growing on

24-12-2025 17:08

Hulda Caroline Holte

Hello, I have found this propoloid ascomycete on

23-12-2025 11:27

Louis DENY

bonjour forum,sur débris de bois de feuillu (acer

20-12-2025 10:49

Mirek Gryc

Hi. „I doubt it is possible to identify this ti

23-12-2025 08:27

Josep Torres Josep Torres

Hello.Small, yellowish ascomata, with very short a

21-12-2025 09:32

Josep Torres Josep Torres

Hello.A tiny ascomycete found embedded in wood in

21-12-2025 21:32

Pol Debaenst

Hello, Garden, Burgweg 19, Veurne, BelgiumOn 10/1

22-12-2025 23:38

Patrice TANCHAUD

Bonsoir, récolte sur un mur en pierre, apothéci

21-12-2025 12:34

Castillo Joseba Castillo Joseba

me mandan el material seco de Galicia (España) 

22-12-2025 00:47

Patrice TANCHAUD

Bonsoir, récolte à proximité du milieu dunaire

« < 1 2 3 4 5 > »
Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul