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09-02-2026 11:42

Åge Oterhals

Hi forum, I found this Lachnum on old hardwood tw

09-02-2026 14:46

Anna Klos

Goedemiddag, Op donderdag 5 februari vonden we ti

02-02-2026 21:46

Margot en Geert Vullings

On a barkless poplar branch, we found hairy discs

25-01-2026 23:23

Tomaz Vucko Tomaz Vucko

Hello! I found this species that resembles Delitsc

06-02-2026 01:14

Tomaz Vucko Tomaz Vucko

Hello!How would you name this species? Most perith

04-08-2014 17:35

Leandro Sánchez Leandro Sánchez

Sur branche de feuillus, jusqu'à 22 mm diamètre,

05-02-2026 15:07

Vasileios Kaounas Vasileios Kaounas

Found on a fallen needle of Pinus halepensis, diam

05-02-2026 06:43

Stefan Blaser

Hello everybody, Any help on this one would be mu

18-08-2025 15:07

Lothar Krieglsteiner Lothar Krieglsteiner

.. 20.7.25, in subarctic habital. The liverwort i

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul