Accès membres

Mot de passe perdu? S'inscrire

29-06-2016 15:18

Per Vetlesen

HiIt was found on the bark of a dead branch of Jun

07-01-2018 22:47

Per Vetlesen

Grown in moist chamber on bark/resin of fallen Pin

06-04-2026 15:04

David Chapados David Chapados

Hi! Could someone help me identifying this specim

06-04-2026 21:36

Viktorie Halasu Viktorie Halasu

Hello, could anyone please send me the article wi

06-04-2026 19:40

David Gibbs David Gibbs

Help with this one much appreciated, on rotting Fa

06-04-2026 11:07

Louis DENY

Bonjour forum, Trouvé sur bois de feuillu très d

06-04-2026 16:24

Juuso Äikäs

Last Tuesday I found some tiny white Helotiales gr

05-04-2026 13:33

Sylvie Le Goff

Bonjour à tousPuis avoir votre avis sur ce champi

05-04-2026 20:40

Robin Isaksson Robin Isaksson

Hi!Found i Japan on bark of Abies sp. Spores 35-4

06-04-2026 08:15

Lothar Krieglsteiner Lothar Krieglsteiner

some days ago, on the lower surface of leaf of Que

« < 1 2 3 4 5 > »
Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul