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30-03-2026 12:18

Sylvie Le Goff

BonjourRécolté sur la base de Pteridium aquilinu

31-03-2026 08:19

Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,Pourriez-vous m'aider à

30-03-2026 12:03

William Slosse William Slosse

Hello all,On 27/03/26, in Kraaiveld in Wingene (Be

25-03-2026 10:35

Hulda Caroline Holte

Hello,I collected this species growing on a dead b

28-03-2026 17:41

Louis DENY

Bonjour forum,Mollisia trouvée sur tige de Molini

30-03-2026 09:53

Yanick BOULANGER

BonjourVoici des petites fructifications poilues s

27-03-2026 10:47

Ã…ge Oterhals

I have tentatively identified this Stictis to S. f

28-03-2026 07:55

Marc Detollenaere Marc Detollenaere

Hello everybody,Yesterday I found a number of whit

26-03-2026 15:31

Ã…ke Widgren Ã…ke Widgren

Hello,I found this one in October last year, on r

27-03-2026 15:23

Gernot Friebes

Hi,this Trichopezizella deviates from typical T. b

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul