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16-02-2026 18:34

Thierry Blondelle Thierry Blondelle

Bonjour,La micro de cet anamorphe de Hercospora su

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

16-02-2026 17:14

Joanne Taylor

Last week we published the following paper where w

16-02-2026 17:13

Joanne Taylor

Last week we published the following paper where w

16-02-2026 16:53

Isabelle Charissou

Bonjour, quelqu'un pourrait-il me transmettre un

16-02-2026 00:05

Maren Kamke Maren Kamke

Good evening, I am looking for the following pape

16-02-2026 11:53

Joeri Belis

between leaf litter on twig in young salix growth.

14-02-2026 22:45

Tomaz Vucko Tomaz Vucko

Hy!I would ask for some help determing this specie

13-02-2026 03:30

Tomaz Vucko Tomaz Vucko

Hello! I found these immersed perithecia on a stic

15-02-2026 20:28

éric ROMERO éric ROMERO

Bonjour tous, Un Mollisia qui me pose problème..

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul