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04-11-2025 14:53

Josep Torres Josep Torres

Hello.Very small, globose, mucronate perithecia, b

08-11-2025 09:15

Michel Hairaud Michel Hairaud

Bonjour, Pouvez vous m'aider à identifier ce Mol

08-11-2025 12:10

Elisabeth Stöckli

Bonjour, Trouvé sur tiges mortes de Rubus (ronce

08-11-2025 00:29

Francois Guay Francois Guay

I found this species in Quebec, Canada, on herbace

06-11-2025 16:50

Rot Bojan

Hello! Yesterday I found a fungus on or near a nee

05-11-2025 11:33

Pierre Repellin

Bonjpur,J'ai trouvé, sur une hampe florale d'Alli

04-11-2025 09:07

Josep Torres Josep Torres

Hello.A suspected Hymenoscyphus sprouting on a thi

04-11-2025 12:43

Edvin Johannesen Edvin Johannesen

Hi! One more found on old Populus tremula log in O

03-11-2025 21:34

Edvin Johannesen Edvin Johannesen

These tiny (0.4-0.5 mm diam.), whitish, short-stip

03-11-2025 19:41

David Chapados David Chapados

Hi,Does anyone knows which genus could this be? G

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Induction of ascomata in pure culture
Björn Nordén, 07-01-2019 12:40
Hi,

I wonder if anyone has experience of induction of ascomata in pure culture? I suspect the methods are fairly species-specific or are there some protocols involving various media, temperatures and light et c that can be used for screening a lot of various ascomycetes? I found these articles so far:


Houbraken J, Dyer PS (2015): Induction of the Sexual Cycle in Filamentous Ascomycetes... In: van den Berg, MA., Maruthachalam, K (Eds.) (2015): Genetic Transformation Systems in Fungi, Volume 2 (Fungal Biology). Springer. DOI: 10.1007/978-3-319-10503-1_2.


Untereiner Wendy A. (1994): A Simple Method for the in Vitro Production of Pseudothecia in Species of Capronia. Mycologia 86, No. 2 (Mar. - Apr., 1994), pp. 290-295


Grateful for any advice! (for example, lab, person, articles).


Björn Nordén

Spooren Marco, 07-01-2019 14:28
Spooren Marco
Re : Induction of ascomata in pure culture
Hello Björn,

Maby the simplest way to find these things out is to write an e- mail to the Westerdijk-Institute.

I had good advices from the kitchen there, that time, they are an open institute and are willingly to help as much they can, is my expierence.

Otherwise there are some, antiquarian books, but the same Westerdijk-institute has a manual about tese things.

Best,

Marco
Björn Nordén, 07-01-2019 14:36
Re : Induction of ascomata in pure culture
Excellent idea! Thank you!
Paul Cannon, 08-01-2019 12:36
Re : Induction of ascomata in pure culture
Two rather brief general hints here:

1. adding the appropriate sterilized substratum to the agar plate (bark, stem, whatever you isolated from) helps to provide a physical base on which ascomata can develop.

2. Don't overfeed your fungus - culture media with high nutrient levels encourage vegetative growth only. Fungi often produce fruit bodies when stressed, when they "think" that their food source is running out.

But there are about as many methods as there are species, so experimentation is inevitable. And do be patient, sometimes fruit body formation can take months...

Best wishes

Paul