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Pezicula sp. on indet. hardwood Appalachian Highl

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Tatraea sp. on indet. hardwood The Swag, Great Sm

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Dear Forum,On a barkless Populus I found some smal

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Hi there, although I couldn't see the fruitbody, I

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Small Hymenoscyphus on Robinia petioles
Manak Roman, 08-01-2019 19:43
Hi all,

in December, I found small Hymenoscyphus on old petioles of Robinia pseudoacacia. Frutibodies had a diameter around 1 mm (the biggest one 1,5 mm) and similar height. The young fruitbodies was yellow, elderly was lighter.

Asci without croziers, IKI+, Mlz+ (without KOH pretreatment), 90-112×7,7-10,7 µm, spores 16,6-24,5×3,7-5,6 µm (measured in fresh state).

Do not you have any tip?

Thanks

Roman
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Hans-Otto Baral, 08-01-2019 20:23
Hans-Otto Baral
Re : Small Hymenoscyphus on Robinia petioles
My guess is H. scutula. A blackish stroma seems to be absent, and spore size looks good. very short setulae seem to be present.

Living paraphyses you did not study?
Bernard Declercq, 09-01-2019 15:18
Bernard Declercq
Re : Small Hymenoscyphus on Robinia petioles
Hi Roman and Zotto,

Based on ascus length a typical H. vitellinus, which I collected a.o. on petioles of Fraxinus. But I do not know if molecular studies  accepted H. vitellinus and H. scutula being separate species.

Bernard
Hans-Otto Baral, 09-01-2019 16:37
Hans-Otto Baral
Re : Small Hymenoscyphus on Robinia petioles
Hi Bernard!
Molecular studies on Hymenosc. scutula are sparse!

Chinese H. scutula (KC416308, KC416309) is Cyathicula coronata (!)

Japanese H. scutula (AB926114) might be good, or more probably 

New York (small town Cuba) H. scutula (KC481695), which has 10 nt distance from the next:

Swiss sample (ZT 4292) not in GenBank which is very close to a Netherlands sample in CBS 101.66


And that's it!

Zotto
Manak Roman, 09-01-2019 19:24
Re : Small Hymenoscyphus on Robinia petioles
Hi Zotto and Bernard,

thank you. My first opinion was Hymenoscyphus scutula too. But in the Beranrd's key is written that apos should have reddening. I did not notice that.

Zotto, I'm sorry, but I forgot to thoroughly explore paraphyses.

Roman
Hans-Otto Baral, 09-01-2019 20:29
Hans-Otto Baral
Re : Small Hymenoscyphus on Robinia petioles
Reddening is a consequence of the presence of striking VB-guttules in the paraphyses, and in most typical Hymenoscyphus species they occur. I think your seond photo shows a reddish hymenium.
Manak Roman, 11-01-2019 20:36
Re : Small Hymenoscyphus on Robinia petioles
Zotto,

thank you for your explanation. There is a hint of redness on a larger picture.

Roman