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Calycina subherbarum
Roland Labbé,
13-12-2008 01:20
Cette espèce est maintenant Calycina subherbarum.
Voici les nouveaux détails :
Date de récolte: 2008.10.25
Habitat: terrain vague
Hôte: tige de plante de plate-bande, échinacée probable
Spores fusoïdes, légèrement arquées, à un septum, lisses, hyalines, guttulées, avec 1 grosse guttule de chaque côté du septum et une moyenne à chaque pôle et parfois quelques petites entre les grosses, 12-17 x 2-3 µm, 14,1 x 2,5 µm en moyenne (10 spores), Q = 5,64
Asques à 8 spores bisériés, avec appareil apical amyloïde, 90-105 x 5-7 µm
Crochets présents à la base des asques
Paraphyses cylindriques, rarement lancéolées, parfois légèrement élargies à l’apex, non ramifiées, à 3-6 septa, avec quelques petites guttules sur la moitié inférieure, hyalines, 100-110 x 2-4 µm
Excipulum ectal en textura angularis, à cellules terminales polymorphes, caténulées, subglobuleuses, clavées, cylindriques, lancéolées, élargies ou déformées à l’apex, ou cystidioïdes-fusoïdes, à paroi épaisse à mince, ochracées en NaCl iso., grises en Melzer
Poils banaux, cylindriques, à paroi mince
Sporée non disponible
Merci, Roland
Hans-Otto Baral,
13-12-2008 10:58
Re:Hymenoscyphus herbarum ?
Hi Roland
yes, this is Calycina herbarum, or I presume subherbarum because I feel the ascus base is with croziers. All parts are dead on your images, anyhow I am sure for herbarum s.l. :-). The ascus reaction is quite strong in (sub)herbarum, but hemiamyloid (RB), therefore weak in Melzer. So if you pretreat with KOH you will get a strong blue reaction in both IKI (LUgol) or Melzer.
Zotto
yes, this is Calycina herbarum, or I presume subherbarum because I feel the ascus base is with croziers. All parts are dead on your images, anyhow I am sure for herbarum s.l. :-). The ascus reaction is quite strong in (sub)herbarum, but hemiamyloid (RB), therefore weak in Melzer. So if you pretreat with KOH you will get a strong blue reaction in both IKI (LUgol) or Melzer.
Zotto
Roland Labbé,
14-12-2008 04:37
Re:Calycina subherbarum
Hi Zotto !
I have replaced the photo by a new one.
After pretreat with KOH, the asques are distinctly IKI bb.
It's easy to see on the photo.
We have found too the croziers at ascus base.
Now, I name it Calycina subherbarum.
But, I would like to know why it is not an Hymenoscyphus.
More, what are the differences between Hymenoscyphus and Calycina ?
Thank you for your precious help.
Friendly ! Roland
I have replaced the photo by a new one.
After pretreat with KOH, the asques are distinctly IKI bb.
It's easy to see on the photo.
We have found too the croziers at ascus base.
Now, I name it Calycina subherbarum.
But, I would like to know why it is not an Hymenoscyphus.
More, what are the differences between Hymenoscyphus and Calycina ?
Thank you for your precious help.
Friendly ! Roland
Hans-Otto Baral,
14-12-2008 12:41
Re:Calycina subherbarum
Hi Roland
I brought an image of the different apical ring types in an earlier request (maybe 2 months ago). On your photo the apical rings look splendid to fit to the Calycina type. Hymenoscyphus type is completely different, much thinner, not extending apically, often reactive only in lower part of the apical wall thickening. Molecular data fully support that herbarum was misplaced in Hymenoscyphus, and several species earlier placed in Pezizella are very very similar to herbarum (f.ex. Calycina parilis).
Zotto
I brought an image of the different apical ring types in an earlier request (maybe 2 months ago). On your photo the apical rings look splendid to fit to the Calycina type. Hymenoscyphus type is completely different, much thinner, not extending apically, often reactive only in lower part of the apical wall thickening. Molecular data fully support that herbarum was misplaced in Hymenoscyphus, and several species earlier placed in Pezizella are very very similar to herbarum (f.ex. Calycina parilis).
Zotto
Roland Labbé,
14-12-2008 16:37
Re:Calycina subherbarum
Thank you Zotto.
I would like tou know again the différences between C. herbarum et C. subherbarum.
Is it only the precence or absence of croziers.
Amitiés ! Roland
I would like tou know again the différences between C. herbarum et C. subherbarum.
Is it only the precence or absence of croziers.
Amitiés ! Roland
Hans-Otto Baral,
14-12-2008 17:09
Re:Calycina subherbarum
This subherbarum is undescribed and it is almost only the croziers which differs. So one could also merge the two in one species. But populations were so far consistent, either + or -. The guttules are a bit larger in herbarum and the apothecia in subherbarum larger, but these are probably a not very useful and too variable characters. Here my present distinction (the conidia in herbarum I seem to have not measured yet):
27. A. with croziers, Sp. oil content 2-4, LBs medium large: 0,5-1,8(2,1) µm, Ap. 0,7- 3,5 mm, Sp. form conidia 4,5-7/1,3-1,7 µm .................. C. "subherbarum"
27. A. without croziers, Sp. oil content 4-5, LBs larger: 1,5-2,5 µm, Ap. 0,4-1,2 mm, Sp. form conidia ................................................ C. herbarum
Zotto
27. A. with croziers, Sp. oil content 2-4, LBs medium large: 0,5-1,8(2,1) µm, Ap. 0,7- 3,5 mm, Sp. form conidia 4,5-7/1,3-1,7 µm .................. C. "subherbarum"
27. A. without croziers, Sp. oil content 4-5, LBs larger: 1,5-2,5 µm, Ap. 0,4-1,2 mm, Sp. form conidia ................................................ C. herbarum
Zotto
Roland Labbé,
15-12-2008 04:45
Re:Calycina subherbarum
Mr. Barral, may I have this key of Calycina.
This will be very usefull to me.
Friendly ! Roland
This will be very usefull to me.
Friendly ! Roland
Hans-Otto Baral,
15-12-2008 11:23
Re:Calycina subherbarum
you have it on the DVD. Open directory CA Keys, and here Helotiales key english. The key starts with
H Y A L O S C Y P H O I D E A E
ANTINOA, CALYCELLINA (Cll.), CALYCINA (C.), PSEUDOHELOTIUM
good luck
Zotto
H Y A L O S C Y P H O I D E A E
ANTINOA, CALYCELLINA (Cll.), CALYCINA (C.), PSEUDOHELOTIUM
good luck
Zotto
Roland Labbé,
15-12-2008 12:57
Re:Calycina subherbarum
OK, I have found it,
Thank again, Roland
Thank again, Roland
Jacques Landry,
21-12-2008 02:27
Re:Calycina subherbarum
Zotto,
Where were the molecular data that herbarum was misplaced in Hymenoscyphus published?
Where were the molecular data that herbarum was misplaced in Hymenoscyphus published?
Hans-Otto Baral,
23-12-2008 20:19
Re:Calycina subherbarum
Hi Jacques
I remember only an unpublished phylogenetic analysis of Spanish workers. It was presented on the EMC in Alcala de Henares in 1999. Calycina herbarum clusters there with Bisporella citrina, Pezizella languida, Calycellina albida and others, while Hymenoscyphus species form a clade of their own, quite remote of Calycina/Calycellina.
Also Verkley accepted placement in Calycina/Pezizella based on the ultrastructure of the apical ring, which is, in my opinion, also clearly visible with the light microscope.
Zotto
I remember only an unpublished phylogenetic analysis of Spanish workers. It was presented on the EMC in Alcala de Henares in 1999. Calycina herbarum clusters there with Bisporella citrina, Pezizella languida, Calycellina albida and others, while Hymenoscyphus species form a clade of their own, quite remote of Calycina/Calycellina.
Also Verkley accepted placement in Calycina/Pezizella based on the ultrastructure of the apical ring, which is, in my opinion, also clearly visible with the light microscope.
Zotto
Jacques Landry,
23-12-2008 20:35
Re:Calycina subherbarum
Here is what I found showing that Calycina herbarum is clearly not in the Hymenoscyphus clade.
Wen-Ying Zhuang & Chao-Yang Liu, Taxonomic reassessment of two taxa of helotialean fungi
MYCOTAXON 99: 123-131 January-March 2007
Wen-Ying Zhuang & Chao-Yang Liu, Taxonomic reassessment of two taxa of helotialean fungi
MYCOTAXON 99: 123-131 January-March 2007