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22-02-2020 16:41

Robin Isaksson Robin Isaksson

I wonder if this one is Hysterobrevium constrictum

05-02-2015 11:21

Zoltan Lukacs Zoltan Lukacs

Hi all, has someone a PDF of Mycographia, seu Icon

21-02-2020 20:43

Thorben Hülsewig

Hi there,I found this fungus on a branch (probably

22-02-2020 07:20

Mirek Gryc

Hi.It is a popular species but I have not been abl

21-02-2020 20:33

Rubén Martínez-Gil Rubén Martínez-Gil

Hola a todos. Subo unas fotos de un Ascobolus que

22-02-2020 08:22

Vasileios Kaounas Vasileios Kaounas

First time i see so little Geoglossum, 1 cm. Beca

21-02-2020 14:47

Savic Dragisa

Hi to all, I need help identifying this lichenicol

14-02-2020 15:27

Savic Dragisa

HiThey're not fungi (but who knows, maybe they are

21-02-2020 11:48

Michel Hairaud Michel Hairaud

Bonjour / Hi to everyone Le 1er numéro 2020 d'As

30-03-2013 21:25

François Valade François Valade

HelloA small yellow Bryoscyphus sp? on moss, Pleur

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Sclerotinia sclerotiorum Spores adhesion
Ale Ale, 13-02-2020 16:34
Hi all,

I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.

Attached is a photo of stained spores. Thank you!
  • message #61493
David Malloch, 13-02-2020 17:56
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale, 13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,

Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.

Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch, 13-02-2020 19:32
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.