10-06-2026 23:08
éric ROMERO
Bonjour tous, Je vous propose un Mollisia trouvé
10-06-2026 12:54
Steve ClementsBonjour encore, Pouvez-vous m'aider, s'il vous pl
09-06-2026 18:32
Camille MertensSur morceau de roseau immergé 0,5 - 0,7 mm de dia
10-06-2026 21:16
François Freléchoux
Bonsoir,Le dernier du jour, en attendant votre avi
10-06-2026 21:07
François Freléchoux
Toutes les tiges de gentianes jaunes de l'an passÃ
10-06-2026 13:41
François Freléchoux
Bonjour à nouveau, Voici une trouvaille d'hier.
10-06-2026 11:53
Steve ClementsBonjour, This disco is abundant on dead stems of
10-06-2026 10:45
François Freléchoux
Bonjour à nouveau, Encore une détermination qui
08-06-2026 10:16
Spooren Marco
I don`t have a clou about this fungus,it is not in
10-06-2026 09:24
François Freléchoux
Bonjour, J'imagine que cette détermination ne do
Sclerotinia sclerotiorum Spores adhesion
Ale Ale,
13-02-2020 16:34
Hi all,
I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.
Attached is a photo of stained spores. Thank you!
David Malloch,
13-02-2020 17:56
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale,
13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,
Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.
Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch,
13-02-2020 19:32
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.