31-03-2026 21:18
Miguel Ángel Ribes
Good evening. oes anyone have the original descrip
31-03-2026 20:57
Stefan BlaserHello everybody, I hope somebody can help me with
26-03-2026 15:31
Åke Widgren
Hello,I found this one in October last year, on r
31-03-2026 16:20
Mlcoch Patrik
Hello, Please about help with determination. On
31-03-2026 08:19
Bernard CLESSE
Bonjour à toutes et tous,Pourriez-vous m'aider à
30-03-2026 12:03
William Slosse
Hello all,On 27/03/26, in Kraaiveld in Wingene (Be
25-03-2026 10:35
Hulda Caroline HolteHello,I collected this species growing on a dead b
30-03-2026 09:53
Yanick BOULANGERBonjourVoici des petites fructifications poilues s
Sclerotinia sclerotiorum Spores adhesion
Ale Ale,
13-02-2020 16:34
Hi all,
I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.
Attached is a photo of stained spores. Thank you!
David Malloch,
13-02-2020 17:56
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale,
13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,
Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.
Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch,
13-02-2020 19:32
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.