18-05-2026 12:43
Sylvie Le GoffBonjour à tousPuis je avoir votre aide sur ce que
18-05-2026 10:13
Lieve Deceuninck
Dear forum members,I identified this as the teleom
17-05-2026 19:05
Thomas FlammerI have found this tiny 200 ym cup shaped apothecia
17-05-2026 16:41
Margot en Geert VullingsWe found this Lachnum on an old Rubus stem.Fruitbo
05-04-2026 22:46
Lothar Krieglsteiner
on wood of Ceratonia, Algarve, 3.4.2026.The color
15-05-2026 13:33
Sylvie Le GoffBonjour à tousJe serais très reconnaissante enve
16-03-2011 14:31
roman vargas albertoHi. I would like some opinion about this Peziza
14-05-2026 05:36
Ethan CrensonHi all, I haven't paid much attention to Lachnu
Sclerotinia sclerotiorum Spores adhesion
Ale Ale,
13-02-2020 16:34
Hi all,
I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.
Attached is a photo of stained spores. Thank you!
David Malloch,
13-02-2020 17:56
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale,
13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,
Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.
Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch,
13-02-2020 19:32
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.