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03-09-2020 19:34

Mirek Gryc

HiAs in the title: on the parts of the Viscum albu

17-09-2020 11:07

Joop van der Lee Joop van der Lee

Normally Pilobolus are found on horse dung but thi

22-09-2020 18:27

Mirek Gryc

HiPlease give me a hint.Mirek

22-09-2020 15:22

Savic Dragisa

Hello everyoneI need help with this anamorph. Lea

20-09-2020 18:11

Castillo Joseba Castillo Joseba

De esta mañana  sobre madera posiblemente de Fag

21-09-2020 08:57

Castillo Joseba Castillo Joseba

De ayer en madera de FagusPelos hasta 1533µA ver

21-09-2020 12:36

Castillo Joseba Castillo Joseba

De ayer en rama de avellano (Corylus),   crfeo q

22-09-2020 12:15

Joop van der Lee Joop van der Lee

Found on dung pallets of roe deer.No living sprang

21-09-2020 15:55

Andgelo Mombert Andgelo Mombert

Bonjour à tous, Avec Gilbert, nous recherchons l

21-09-2020 20:34

Marcel Heyligen Marcel Heyligen

Hello anybodyI need help with the determination o

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Sclerotinia sclerotiorum Spores adhesion
Ale Ale, 13-02-2020 16:34
Hi all,

I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.

Attached is a photo of stained spores. Thank you!
  • message #61493
David Malloch, 13-02-2020 17:56
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale, 13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,

Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.

Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch, 13-02-2020 19:32
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.