27-11-2025 15:41
Thomas LæssøeSpores brownish, typically 4-celled; 26.8 x 2.4;
27-11-2025 12:01
Thomas Læssøehttps://svampe.databasen.org/observations/10496727
27-11-2025 11:46
Thomas Læssøehttps://svampe.databasen.org/observations/10493918
27-11-2025 11:31
Thomas LæssøeCollectors notes: Immersed ascomata, erumpent thro
23-09-2025 13:31
Thomas Læssøehttps://svampe.databasen.org/observations/10534623
25-11-2025 14:24
Thomas Læssøehttps://svampe.databasen.org/observations/10490522
26-11-2025 18:13
Jason Karakehian
The entire run of Mycotaxon is now available throu
25-11-2025 11:03
Mick PeerdemanHi all,One of my earliest microscopy attempts, so
Sclerotinia sclerotiorum Spores adhesion
Ale Ale,
13-02-2020 16:34
Hi all,
I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.
Attached is a photo of stained spores. Thank you!
David Malloch,
13-02-2020 17:56
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale,
13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,
Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.
Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch,
13-02-2020 19:32
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.