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23-05-2026 11:44

Coprophilous pyrenomycete wild boar dung
Charles Grapinet Charles Grapinet

Hello, I am having trouble identifying this copro

25-05-2026 16:44

Ascomata under epidermis Carex
François Bartholomeeusen

Hi forum members,During an excursion organised by

26-05-2026 21:25

small ascomycetes on Quercus sp.
Dirk Gerstner

Hello everyone, I'm completely stumped by this li

26-05-2026 22:44

Incrucipulum capitatum ?
Ethan Crenson

Hi all, I think I have Incrucipulum capitatum her

22-05-2026 14:44

minute Saccobolus from cow-dung (moist chamber from Portugal)
Lothar Krieglsteiner Lothar Krieglsteiner

in unripe condition citrine yellow, then soon fadi

25-05-2026 16:35

Anamorphe d'Hypomyces aurantius ? sous saule cendré (Salix cinerea)
Bernard CLESSE Bernard CLESSE

Bonjour à toutes et tous,J'ai trouvé récemment,

22-05-2026 13:29

Pseudophacidium ?necans on Vaccinium myrtillus
Gernot Friebes

Hi,I am curious to hear your opinion on this mater

23-05-2026 18:57

Mollisia scopiformis
Sylvie Le Goff

Bonjour à tousRécolté sur une branchette de Sal

23-05-2026 23:53

cf Godronia sp.
Moreno Miriam

Bonjour ! Je travaille sur mon mémoire de master

22-05-2026 21:35

A yellow synnemata (coremia) on wood
Steve Clements

Bonjour, I expected this find on old wood on our

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Sclerotinia sclerotiorum Spores adhesion
Ale Ale, 13-02-2020 16:34
Hi all,

I am working with SS spores on glass surfaces. My current problem is that they get stuck to the surface of glass very easily and they also tend to form clumps. I already tried surfactants such as Tween 20, Pluronic, PVA but I was not able to solve the problem. Any advice, tips or suggestions?.

Attached is a photo of stained spores. Thank you!
  • message #61493
David Malloch, 13-02-2020 17:56
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
I have found that the spores of many white-spored agarics, especially Mycena, stick to glass slides in small clumps and are difficult to dislodge. Usually a drop of water will cause them to rehydrate and separate, but in some situations oil (mineral oil or even olive oil) seems to work. If you wish to make single-spore isolates, it would probably be better to discharge the spores on to an agar surface.
Ale Ale, 13-02-2020 19:13
Re : Sclerotinia sclerotiorum Spores adhesion
Hi David,

Thank you for your answer. I think I should explain a bit better my problem. Currently I have the spores flowing in a microchannel with DI water, so spores are already in contact with water. The flow is generated using a micropump. Some spores get stuck to the surface of this microchannel (glass) and wont move even at high flow rates. I noticed this problem not only in glass but in surfaces such as: Aluminum, Gold, PDMS, SU-8.

Spores get stuck easily and the surfactants I mentioned before did not help.
David Malloch, 13-02-2020 19:32
David Malloch
Re : Sclerotinia sclerotiorum Spores adhesion
This is beyond anything I have experienced. It would seem that the spores are genetically disposed to stick firmly to any surface they encounter; not a bad strategy for a plant parasite. Perhaps the "glue" is impervious to most normal surfactants or solvents. That sounds like a challenging and interesting problem.