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Apiospora montagnei telemorph state alongside Arthrinium state
Hardware Tony, 12-06-2020 19:59
Hardware TonyFound on Bambusa, I first thought these were two different species. After research, I think that A. montagnei is showing the teleomorph state and spores and the same species is showing the Arthrinium state with anamorph spores. They were within six inches of each other on the same dead stick of Bambusa. Can anyone confirm this is the case or they are two distinct species?
With thanks  Tony Hardware
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Angel Pintos, 12-06-2020 21:10
Angel Pintos
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Hi Tony,
Both states could be be the same species, but with the measures of the conidia of your images, surely it is not the asexual morph of Apiospora montagnei as we understand it today.
Knowing the measurements of the conidia, I would say that it is A. phragmites or A. marii.
can you measure the ascospores?
Best wishes 
Angel
Hardware Tony, 13-06-2020 14:02
Hardware Tony
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Hello Angel,
Many thanks for looking at this, expect you are correct in your observations as looking again A. arundinis the conidia spores seem too small at around 5µm or so. I added another pic here taken at 400x showing more conidia which suggests from the size and looking at the majopiry I may have measured the oversized conidia spores more than the rest and looks at around 8µm plus. This suggests A. marii more than A. phragmites perhaps, also A. Marii has been found on bambusa before whereas I could find no ref to the other except on traditional grasses. A Marii macro size is much closer at 2-6mm, as there were quite large stromata slits rather than the A. phragmite is much smaller. I checked at ref articles at: https://mycokeys.pensoft.net/article/32115/  and 
https://www.researchgate.net/publication/253340268_A_phylogenetic_re-evaluation_of_Athrinium   I'm no expert for sure and was astounded to see the different species in this genus that could be found. Apprecaite your lead on this and hopefully going somewhere closer to the right ID.   Tony
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Hardware Tony, 13-06-2020 14:13
Hardware Tony
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Angel,Also I couldn't measure the ascospores, none found at the time.  Tony
Angel Pintos, 13-06-2020 15:11
Angel Pintos
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Hi, Tony,

In the article you mention, it shows that different stages of different Arthrinium species can cohabit in the same culm. For this reason we cannot be sure that the conidia  you show in the photographs are of the same species as the stroma of the photographs and therefore we cannot link both stadiums.

We could only link it by isolating the ascospores and comparing the conidia resulting from the culture.

It would be nice to know the measurements of the ascospores because that could help to discard or confirm it, without being definitive.

It is very complex to identify some species of Arthrinium, especially those that have an sexual morph Apiospora-like because the measurements of the ascoporas and the measurements of the conidia overlap and makes it almost impossible to identify it. This is also mentioned in the article by Crous 2013, and before Samuels 1981.

Best wishes

Angel Pintos
Angel Pintos, 13-06-2020 20:49
Angel Pintos
Re : Apiospora montagnei telemorph state alongside Arthrinium state
If you put some stromata in water for one night or one day it  probably produce ascospores
Hardware Tony, 14-06-2020 15:21
Hardware Tony
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Hello Angel,
Firstly, apologises didn't see you were one of the key authors of the paper I quoted. I have learnt a great deal already about this species and next time I will endeavour to match the ascospores with conidia as you suggest. No luck this time, but getting closer perhaps. 
Many thanks for your help, the article was very helpful.

Tony
Angel Pintos, 14-06-2020 18:13
Angel Pintos
Re : Apiospora montagnei telemorph state alongside Arthrinium state
Hi Tony,
you are wellcome