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23-01-2021 19:42

Sven Heinz Sven Heinz

Hello, i found this fungi on fallen Betula twigs.

24-01-2021 16:13

Pablo Sandoval Pablo Sandoval

Hola amigos!Encontré esta especie de Lamprospora

24-01-2021 20:21

Hardware Tony Hardware Tony

I found under Picea albies branch on the ground a

22-01-2021 06:21

Laurent LENEL Laurent LENEL

Bonjour à tous, cette pézize semblerait être Pe

24-01-2021 11:13

Castillo Joseba Castillo Joseba

Me mandan el materialseco de Galicia, recolectado

24-01-2021 08:51

Castillo Joseba Castillo Joseba

Me mandan esta especie en seco, recolectada en Cr

22-01-2021 19:09

Mirek Gryc

HiThe collection is from 2016, but I only microsco

23-01-2021 19:04

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello.I'm searching for a PDF copy of the followin

21-01-2021 15:59

Mirek Gryc

Hi.Found a few years ago but so far I do not know

21-01-2021 03:47

Khomenko Igor Khomenko Igor

Hi, I found this small white discomycete on Alnu

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Phaeohelotium (?) on Salix caprea "Kilmarnock"
Edvin Johannesen, 28-11-2020 17:00
Edvin Johannesen
Apothecia short-stipitate, pale yellow, 2-5 mm, orange upon drying. Asci ca. 140x8 microns, narrowly club-shaped. IKI+ (blue with Lugol), but visible only in a few asci. Spores (16)-18-20 x 4-5 microns. Measures of live structures in water. Spores multiguttulate, becoming 1-septate. Many spores germinating with a long, narrow hypha. Paraphyses with septa, of uniform thickness. Subhymenium/excipulum gradually from textura intricata/epidermoidea to globosa/prismatica. No apparent gelatinised structures. Growing on Salix caprea "Kilmarnock".

Are we in Phaeohelotium or Hymenoscyphus? What is separating the two genera these days?

Thanks.
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Edvin Johannesen, 28-11-2020 17:03
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Some more photos.
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Lothar Krieglsteiner, 28-11-2020 17:27
Lothar Krieglsteiner
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Hello Edvin,
perhaps Hymenoscyphus conscriptus?
Best regards, Lothar
Hans-Otto Baral, 28-11-2020 18:18
Hans-Otto Baral
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Or H. subferrugineus. Difficult to say in an overmature stage.

presumably the ectal excipulum is prismatica also at the lower flank.
Edvin Johannesen, 29-11-2020 00:11
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Thanks to both.

I'm a bit confused regarding Hymenoscyphus vs. Phaeohelotium.
Hans-Otto Baral, 29-11-2020 10:08
Hans-Otto Baral
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
This  far from easy! And there is Cudoniella in which perhaps various Phaeohelotiums should be placed.
Edvin Johannesen, 29-11-2020 17:26
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
It's comforting to know that it's not only difficult for me!

I have some younger apothecia that may not be as overmature.  There are only two, old, collections of H. subferrugineus from Norway, so it would interesting to try to verify if this is it. Is there a good way to distinguish H. subferrugineus from H. conscriptus?  I see there are som good images of both species at asco-sonneberg.de. Do they reflect the differences?
Hans-Otto Baral, 29-11-2020 17:49
Hans-Otto Baral
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Yes, but you can also look at my calyculus group folder.

H. conscriptus has a rather low spore lipid content, H. subferrugineus a high content with larger LBs, and spore shape is more distinctly scutuloid (heteropolar, basally pointed).
Edvin Johannesen, 29-11-2020 17:58
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
I was looking in your folder, but somehow I overlooked it. Now I see the subferrugineus images. I will have a second look with these characters in mind. Thanks again!
Edvin Johannesen, 29-11-2020 20:54
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
OK, I'll give it a shot with a few more pictures in Congo Red, this time from a smaller apothecium (still probably a bit overmature since there are germinating spores). My judgement is that this fits H. subferrugineus, but there is always the danger of confirmation bias. Images show spores, subhymenium, ectal excipulum, and ascus base/crozier, in that order. The final image shows how the ascus (with spores inside) and paraphyse contents are coloured in Baral'sche Lösung.

Thoughts still appreciated.
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Mirek Gryc, 30-11-2020 09:26
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Hello all
Although I probably won't help you identify it, let me join the discussion :)
I don't know H. subferrugineus very well, but H. conscriptus is a species so common that I even downplayed it and did not make good documentation for my atlas (sorry, lack of time). I just wanted to mention that I have microscoped at least several dozen of his collections but I have never seen spores with such a rich content. The spores in my collections contained at most a few tiny droplets.
Of course, they were all from Salix spp
best regards
Mirek
Hans-Otto Baral, 30-11-2020 10:00
Hans-Otto Baral
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Yes, it is clearly H. subferrugineus. The only question that remains is: what is H. calyculus? So much as I know is that H. subferrugineus is also genetically a clear species, whereas there exist several different sequences for samples identified as H. calyculus, and it is very long ago that I saw a fresh collection of H. calyculus.

I would appreciate collection daat of the present sample.

Zotto
Edvin Johannesen, 30-11-2020 10:23
Edvin Johannesen
Re : Phaeohelotium (?) on Salix caprea "Kilmarnock"
Thank you so much, Mirek and Zotto! I will send you the colletion details, Zotto, by email, as soon as I have it.