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27-11-2021 02:38

Laurent LENEL Laurent LENEL

Bonjour à tous, voici ce que je pense être Otide

23-11-2021 15:26

Jennifer Fiorentino

First I supsected this was a slime mould but slime

28-11-2021 11:47

Viktorie Halasu Viktorie Halasu

Hello,I'm trying to find a name for this small yel

26-11-2021 15:21

Joop van der Lee Joop van der Lee

Found on dung of roe deer.In detail it differs fro

26-11-2021 13:43

Enrique Rubio Enrique Rubio

Do you have any idea about this coelomycetous fung

25-11-2021 23:14

S. Rebecca

Hello, we are spending some time between Valencia

23-11-2021 23:46

Valencia Lopez Francisco Javier

Hola a todosEsta Lambertella emergían de entre la

25-11-2021 22:25

Zoltan Lukacs Zoltan Lukacs

It was found in a deciduous forest, In Hungary. A

23-11-2021 20:52

Jannes Jannes Boers

Hello Everyone, The passed year there have been s

25-11-2021 13:47

Georges Greiff

Hello,I have been sent an interesting Octospora fr

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Calycellina spiraeae spores
Chris Yeates, 21-10-2021 13:26
Chris Yeates
Bonjour tous

While processing images of this delightful little fungus I noticed that some of the ascospores show a distinct hyaline "sheath". I dismissed the possibility that it was an artefact, largely because it does not show on all the spores photographed in exactly the same way. As you can see it is also evident in the spore seen "end on".

I can easily see that this might be missed by normal light microscopy but wondered whether any other members have noticed this phenomenon?

Cordialement, Chris
  • message #70392
Hans-Otto Baral, 21-10-2021 17:13
Hans-Otto Baral
Re : Calycellina spiraeae spores
Yes, this is a sheath, and it is also visible without DIC :-)

Perhaps the spore surface staines lilac in CRB, which is due to a gel below this sheath.
Enrique Rubio, 21-10-2021 17:37
Enrique Rubio
Re : Calycellina spiraeae spores
I think that these ephemeral sheaths are very frequent in the ascopores just ejected from the ascus in many Helotiales. What happens is that unless they are very conspicuous they go unnoticed.
Chris Yeates, 21-10-2021 20:23
Chris Yeates
Re : Calycellina spiraeae spores
Thank you both for these comments.

Hans-Otto Baral, 21-10-2021 20:51
Hans-Otto Baral
Re : Calycellina spiraeae spores
Strange is that they are no always seen in a species. Just in C. spiraeae I see them in ERD 4803 but not in ERD 5959. The mentioned lilac stain with CRB seems specific for Calycellina (and some distantly related genera), but absent, e.g. in Calycina. For C. spiraeae, however, I see I once tested CRB and it was negative. But whether this is a good Calycellina is not sure (no sequence).
Enrique Rubio, 21-10-2021 20:59
Enrique Rubio
Re : Calycellina spiraeae spores
What concentration of CRB do you use to try to stain the sheaths?
Hans-Otto Baral, 21-10-2021 21:55
Hans-Otto Baral
Re : Calycellina spiraeae spores
I cannot say. There is no high concentration necessary, The medium may look hyaline but the gel on typical Calycellina spores attracts the dye.
Enrique Rubio, 24-10-2021 09:30
Enrique Rubio
Re : Calycellina spiraeae spores
Thanks, Zotto.