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Jason Karakehian
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Bernard Declercq
Good evening,On Oenanthe aquatica we collected a
Sareomycete?
Stip Helleman,
12-06-2024 22:08
Dear all,
a week ago this cushen formed species was collected as a side-catch with Lilapila jurana on Picea resin in Swiss Jura at 1030m. So far I don't get any further as that the thing microscopically resembles the Sarea species wit asci 110-120x 30-35 µm and filed with thousands spores sphaerical of 2µm.
I am open for any suggestion, thanks in advance.
I am open for any suggestion, thanks in advance.
Stip
Stip Helleman,
12-06-2024 22:12
Re : Sareomycete?
some more photos
Hans-Otto Baral,
13-06-2024 10:01
Re : Sareomycete?
Hoi Stip
You mean the black disc on the lower right or these grey warted ones?
Actually I am not sure with the spores. Alternatively the asci are immature and full of LBs? Did you see free spores?
Sarea reacts deep blue in IKI (entire hymenium), probably turning reddish at high concentration..
Stip Helleman,
16-06-2024 23:32
Re : Sareomycete?
Hoi Zotto,
The grey ones, the spores look smaller in phloxin after KOH, more like 1,5 µm. free spores are seen after the ascus is squeezed broken. Of coarse it can be immature (I don't know any young difformis fruitbodies, in the meantime new fruitbodies are growing.
Stip
Hans-Otto Baral,
17-06-2024 10:03
Re : Sareomycete?
This looks like spores, but the macro-appearance is not that of a Sarea.
Perhaps you could test the KOH-pretreated IKI reaction, if the blue obscures the entire ascus or is only a faint reaction which would be untypical of Sarea.
The apos with their small opening look immature but in section they appear exposed.
Stip Helleman,
17-06-2024 23:19
Re : Sareomycete?
Hoi Zotto,
yes the asci are bleuening after boiled KOH and the fruitbodies are growing in very close contact with Sarea difformis so I assume it wil be its premature stage.
Stip
Hans-Otto Baral,
17-06-2024 23:22
Re : Sareomycete?
Ha, this would surprize me, never seen such apos! Did you compare the black ones with the mic?
Hans-Otto Baral,
18-06-2024 08:55
Re : Sareomycete?
Did you have a look at Mitchell et al. 2021? There Sarea is restricted to black ones and Zythia are the yellow-orange ones.
The ectal excipulum in both is a clearly vertically oriented texture, unlike yours. Also the macro is totally differnt from your grey ones.
I also see differences in the paraphyses, being straight, distinctly thicker and densely septate in Sarea.
I would appreciate James Mitchell's opinion.
Zotto
Stip Helleman,
18-06-2024 22:46
Re : Sareomycete?
Hoi Zotto,
the fruitbody of S. difformis on top of the green one.
I did read the article on Sareomycetes but this did not give a clearence on my collection.
Stip
Hans-Otto Baral,
19-06-2024 14:43
Re : Sareomycete?
Phh, I have now problems to follow. Which green one? Your new micros with a living ascus are from the black ones? It is always a mess with these fungi growing so close together.
Stip Helleman,
19-06-2024 21:57
Re : Sareomycete?
Hoi Zotto,
yes the last micro's are from the S. difformis which is in close contact with this unknown species
Stip Helleman,
19-06-2024 22:20
Re : Sareomycete?
Hoi zotto,
according to James Mitchell it should be an undescribed species and it was on ascofrance before.
Stip
Hans-Otto Baral,
20-06-2024 09:24
Re : Sareomycete?
When they are pseudothecia, why is the disc broadly open in section?
Is there any microscopy of the previous find?