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18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

14-07-2025 17:55

Yanick BOULANGER

BonjourAutre dossier laissé en suspendJe viens de

14-07-2025 11:17

Yanick BOULANGER

BonjourJ'ai un dossier Jackrogersella qui est rest

14-07-2025 15:52

Gernot Friebes

Hi,I wanted to share this collection on Rubus idae

14-07-2025 13:37

Gernot Friebes

Hi,do you think this collection could be R. ulmari

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Pyrenopeziza atrata on Angelica silvestris
Thomas Flammer, 28-04-2025 12:51
Substrate: Angelica sylvesris
Spore mass: 8.4 - 11.1 x 2.1 - 2.7 µm - Q: 3.13 - 4.54 (Ø LxB: 9.4 x 2.4 ØQ:3.9 N: 18)
Spore shape: fusiform
Paraphyses: septated
Clamps: yes
Barals: Apical ring IKI+
Determined by: Microfungi on land plants - Ellis, Martin B; Ellis, Pamela J - The Richmond Publishing Co. Ltd. - 978-085546-246-8
  • message #82419
  • message #82419
  • message #82419
Michel Hairaud, 28-04-2025 20:17
Michel Hairaud
Re : Pyrenopeziza plicata on Angelica silvestris
Bonsoir Thomas, Je ne connais pas cette espèce et ne connais pas de littérature récente la mentionnant. 
Pour être certain qu il s'agit d'un Pyrenopeziza et non d'un Mollisia, il faut s'assurer que les paraphyses n'ont pas de contenu vacuolaire homogène. Je crois le deviner sur la photo micro, mais pas certain. 

Sur ce même support, je suis arrivé parfois à P. atrata , qui est peut être une espèce collective. 

AmitiésMichel
Hans-Otto Baral, 29-04-2025 08:56
Hans-Otto Baral
Re : Pyrenopeziza plicata on Angelica silvestris
Michel is right. To me it looked like a Pyrenopeziza, but I am not sure. If you still have the fungus fresh, please try a section or a soft squash mount in order to see living asci and paraphyses. Only then you have a chance to observe the refractive elongated vacuoles characteristic of Mollisia.
Thomas Flammer, 29-04-2025 10:32
Re : Pyrenopeziza plicata on Angelica silvestris
I added a picture of the paraphyses in water. There really is some refractive content.
Hans-Otto Baral, 29-04-2025 10:36
Hans-Otto Baral
Re : Pyrenopeziza plicata on Angelica silvestris
This is good, it is an SCB, not a VB. This means that when you add Cresyl Blue you will get no stain. SCBs are not vacuolar, unlike VBs. Such globose SCBs are typical of Pyrenopeziza, also the lanceolate shape of the paraphyses are not uncommon in that genus..
Thomas Flammer, 29-04-2025 10:54
Re : Pyrenopeziza plicata on Angelica silvestris
Can I also use Patentblau or Baumwollblau? Is there a need to get Cresyl blue?
Hans-Otto Baral, 29-04-2025 10:56
Hans-Otto Baral
Re : Pyrenopeziza plicata on Angelica silvestris
You must use an aqueous stain, otherwise you will kill the cells immediately. Cotton blue is o.k. but not the one in lactophenol. If you have the powder you can make a solution in water. Patent blue is perhaps similar. Cresyl blue has the advantage to be metachromatic, changing the colour from turquoise to lilac, I think cotton blue doesn't.
Thomas Flammer, 29-04-2025 11:11
Re : Pyrenopeziza atrata on Angelica silvestris
Sorry to bother you again with my questions. But I am quite confuesd with the use of the 4 stains. I have also Toluidinblau, but I have never used it. So what is the "Best blue". Cresyl? Do I really need 4 "Blues". Cresyl blue seems quite interesting due to the metachromatic properties.
Hans-Otto Baral, 29-04-2025 11:45
Hans-Otto Baral
Re : Pyrenopeziza atrata on Angelica silvestris
I do not fully remember, but it can be that Toluidine blue also gives the metachromatic colour change. I prinicipally use Cresyl Blue, it was the stain used by Chadefaud and Le Gal as I remember.

I should asdd that CRB slowly enters the cells as all vital dyes, and a very slight addition of an acid facilitates the transfer.

Cotton Blue/lactic acid is surely helpful, but not for the purpose of accumulation and metrachromasy of living cell contents, which depends on the semipermeable plasmalemma and tonoplast.