13-05-2026 15:26
François Freléchoux
Bonjour,Voici une récolte faite il y a quelques j
12-05-2026 15:41
Nicolas VAN VOOREN
Dear Ascolovers, especially interested in Pezizale
13-05-2026 12:05
Thierry Blondelle
Bonjour à tous,J'aimerais avoir confirmation de c
11-05-2026 12:32
Bernard CLESSE
Pourriez-vous m'aider à identifier cette héloti
10-05-2026 23:17
Andreas Gminder
Hello,today we found in a moist steep decidous for
28-04-2026 20:07
Lothar Krieglsteiner
... on twig in the air at standing Ceratonia siliq
27-04-2026 20:52
Lothar Krieglsteiner
Found on hanging tiwg of Olea europaea in dried-ou
11-05-2026 20:22
Lothar Krieglsteiner
on attached twig of standing Ficus caricaquite uns
29-04-2026 10:44
Lothar Krieglsteiner
growing at moist, drying-out soil at the side of a
Hymenoscyphus?
Josep Torres,
23-12-2025 08:27
Hello.Small, yellowish ascomata, with very short and rudimentary stalks, greenish in mature specimens, sprouting from the surface of decaying leaves of Quercus rotundifolia.
Only 0.2 to 0.4 mm in diameter.
Basal hyphae elongated, arranged parallel to each other, and pigmented.
Hyphae of the excipulum globose-angularis in texture.
Octosporous asci, with an amyloid reaction in their apical apparatus, and although difficult to observe in some of these asci, I thought I saw croziers. Fusiform ascospores with pointed ends, two or three large lipid droplets and several smaller ones scattered throughout, measuring in water:
(14.3) 16 - 19.3 (22) × (2.8) 3.1 - 4.3 (4.5) µm
Q = (4.2) 4.3 - 5.5 (5.7) ; N = 40
Me = 17.8 × 3.6 µm ; Qe = 4.9
Based on these characteristics, I think it could be a species of the Hymenoscyphus/Phaeohelotium complex, but I have no other suggestion that convinces me.
Any opinions you may have would be welcome.
Thank you in advance.
Best regards.
Hans-Otto Baral,
23-12-2025 10:57
Re : Hymenoscyphus?
You don't have living paraphyses? That would be helpful. Surely no Hymenoscyphus, the apical ring is more of the Calycina type. The brown excipulum is remarkable. There are no hairs?
Josep Torres,
23-12-2025 14:22
Re : Hymenoscyphus?
Thanks, Zotto.
I couldn't observe any structures in the prepared samples that could correspond to hairs. The closest thing were the terminal hyphae visible above the image of the excipulum, which might correspond to marginal hyphae. There were no live paraphyses either. The apothecia were past their prime, and there were only asci and a collapse of spores. Since I have quite a bit of material from this sample, if we can't reach any conclusions, I'll send it for sequencing in my next shipment, which would be next year.
Best regards.
I couldn't observe any structures in the prepared samples that could correspond to hairs. The closest thing were the terminal hyphae visible above the image of the excipulum, which might correspond to marginal hyphae. There were no live paraphyses either. The apothecia were past their prime, and there were only asci and a collapse of spores. Since I have quite a bit of material from this sample, if we can't reach any conclusions, I'll send it for sequencing in my next shipment, which would be next year.
Best regards.
Hans-Otto Baral,
23-12-2025 14:57
Re : Hymenoscyphus?
That would be interesting to obtain DNA. It looks a bit like a Hyphodiscus. But such big spores are unknown there.
Josep Torres,
07-03-2026 22:46
Re : Hymenoscyphus?
Hi Zotto.
The sequence came back slightly noisy and didn't clarify much, but the ITS gene sequencing points with a 97.66% match to Calycellina sp., or at most Pezicula.
Best regards.
The sequence came back slightly noisy and didn't clarify much, but the ITS gene sequencing points with a 97.66% match to Calycellina sp., or at most Pezicula.
Best regards.
Hans-Otto Baral,
08-03-2026 08:34
Re : Hymenoscyphus?
Could you please send me the sequence and especially the chromatogram (ab1), then I will try my alignments wit partly unpublished sequences.
Josep Torres,
08-03-2026 14:35
Re : Hymenoscyphus?
Hi Zotto.
They're already in your email.
Best regards. Josep
They're already in your email.
Best regards. Josep
Hans-Otto Baral,
08-03-2026 21:29
Re : Hymenoscyphus?
Thanks, yes, the sequence is noisy, but only at a few positions. The result is clear: It must be a Pezizellaceae. Pezicula is a misID.
I tested ITS and S1506 intron separately:
ITS: 98.5% "Pezicula sp." UDB04294927, 98% Mollisina sp. UDB04221998, 97.8% UDB04222001, many Mollisina sp. 97.4% UDB, 96% Scleropezicula alnicola,
Intron: 91% Helotiales sp. LC064903, 86% Calycellina fagina, 81% Nagrajchalara
I think I see marginal hairs with short somewhat hooked whip. I am also sure there have been VBs inside the hair cells. So Mollisina or especially Calycellina are good options.