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Trematosphaeria pertusa
Björn Wergen, 10-03-2013 11:10
Björn WergenHi again,

do you also think that this collection can be T. pertusa? I have found several articles and photos of this species but because of several contrarieties I do not really know how T. pertusa should look like. The spores of this collection are hyaline and 1septate in immature state, but they become 3 septate and pale greenish to greenbrown.

Macroscopically the perithecia are about 0,4-0,7 mm broad and sphaerical, with a pointed Ostiolus and covered with brown hairs (?).

Collected on attached twigs of Tilia cordata.

regards,
björn
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Yannick Mourgues, 10-03-2013 11:39
Yannick Mourgues
Re : Trematosphaeria pertusa
Hi Björn .

I think it's not T. pertusa because in this specie (see Zhang & al 2008, Are Melanomma Pulvis-pyrius and Trematosphaeria pertusa congeneric ? - Fungal Diversity 33 : 47-60) spores are larger and longer, and dark brown :

"27.5-32.5x7,5-8,5 , fusiform with broadly to narrowly rounded end, dark brown, 1(-3) septate, (...), smooth to finely verruculose, without gel sheat"

Have your spores a gel sheat ?

May be you should make a vertical section of peridium to see cells structure.
Have you looked for in Massarina or Herpotrichia (subiculum ?) species ?

Yannick
Björn Wergen, 10-03-2013 11:56
Björn Wergen
Re : Trematosphaeria pertusa
Hi Yannick,

Herpotrichia is a good idea. But it is not H. herpotrichoides since I had this species already on Rubus sp. with different characters. I had also look for Massarina but I did not find any matching species.
I did not see a gel coat surrounding the spores.

regards,
björn
Björn Wergen, 10-03-2013 14:25
Björn Wergen
Re : Trematosphaeria pertusa
Here are some more data, especially a cut through a perithecia.

Some of them have longer Ostioles and are crowded together. I have seen no gel coat, also not with Indian Ink.

Wall is 25-32µm thick.

regards,
björn
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Ying Ying Zhang, 11-03-2013 03:13
Re : Trematosphaeria pertusa
??Hi Bjorn,
This species do not fit T. pertusa well. The ascospore of T. pertusa is pigmented. The ascomata do not covered with anything ("naked"). The ostiole (opening) is wide. But  the shape of ascospores and asci of this collection are very similar with T. pertusa. I would like to take it as another species of Trematosphaeria s. s. As Trematosphaeria seems not rare in Europe, thus close relatives might also not rare.

Cheer up!
Ying
Björn Wergen, 11-03-2013 21:40
Björn Wergen
Re : Trematosphaeria pertusa
Hi Ying,

thanks for the respond. I have recently found this species again, on Ulmus.

regards,
björn
Björn Wergen, 08-05-2013 16:44
Björn Wergen
Re : Trematosphaeria pertusa
I have found it again, this time on decorticated Acer stems (~5cm).

The spores are a bit smaller than in the other 2 collections, and I haven't seen any spores with more than 1 septa.
As already seen in the other photos, this collection also had a hyphal layer on the small, black perithecia (diam. ~ 0,6mm, beaked).

I have called it "Trematosphaeria lignicola" for now.

I will add it to my special herbarium for further studies.

regards,
björn
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