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13-06-2025 09:41

Josep Torres Josep Torres

Hello.A cerebriform ascomycete sprouting scattered

13-06-2025 16:34

Andgelo Mombert Andgelo Mombert

Bonjour,Un petit discomycète qui me résiste. Il

24-07-2025 19:02

Castillo Joseba Castillo Joseba

De esta mañana en tronco caido de Haya A ver si

21-07-2025 19:22

Ethan Crenson

Hello all,  Here is an Orbilia found by a friend

14-07-2025 11:20

Michel Hairaud Michel Hairaud

Bonjour, Voici une espèce de  (?) Hyaloscyphace

18-07-2025 23:03

Josep Torres Josep Torres

Hello.Fruitings between 51 and 130 microns in tota

17-07-2025 11:55

Castillo Joseba Castillo Joseba

De ayer en bosque de hayas y abetos, en tieraEjemp

16-07-2025 17:34

Bernard Declercq Bernard Declercq

Hello,I have trouble distinguishing above mention

15-07-2025 13:27

Angel Pintos Angel Pintos

Hello, does anyone have access to the following ar

16-01-2023 21:31

Riet van Oosten Riet van Oosten

Hello, Nearby the find of Calycina claroflava on

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Aquatic ascomycete.
Josep Torres, 13-06-2025 09:41
Josep TorresHello.
A cerebriform ascomycete sprouting scattered on the surface of a trunk submerged in a clear stream.
Ascomata measuring up to 4 mm long and 2 to 2.5 mm wide, I was particularly struck by how tough and tenacious these ascomata were.
Very thin conidiophores arranged parallel in a palisade-like pattern produce globose conidia with a large lipid droplet inside that occupies three-quarters of the spore.
An amyloid reaction is observed from the base to the middle of the hymenium with Melzer's Reagent.
No apparent reaction to KOH.
Some of the images show clusters of these supposed conidia, which I wouldn't completely rule out asci.
These presumed conidia measured in water:
(3.6) 3.8 - 4.4 (4.7) × (2.9) 3.2 - 3.8 (4) µm
Q = 1 - 1.3 (1.4) ; N = 40
Me = 4.1 × 3.6 µm ; Qe = 1.1
I admit I don't even know where the air is coming from.
Any opinions would be welcome.
Thank you very much in advance.
Best regards.
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Josep Torres, 17-06-2025 14:09
Josep Torres
Re : Aquatic ascomycete.
Any suggestions.......
Josep Torres, 20-07-2025 14:34
Josep Torres
Re : Aquatic ascomycete.
Hello.
In case anyone is interested, the results of the molecular analysis of the ITS gene have determined that it is an anamorph of Ascocoryne sp. Based on the current data available in Genbank, we cannot be more precise.
I'm adding the phylogenetic tree.
Best regards.
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Francois Guay, 22-07-2025 18:39
Francois Guay
Re : Aquatic ascomycete.
Very interesting, thank you for the follow-up!
Hans-Otto Baral, 26-07-2025 09:29
Hans-Otto Baral
Re : Aquatic ascomycete.
Hi Josep
by accident I looked again in your thread. What a shame that I did not realize this being an Ascocoryne! Now the things are clear thanks to your DNA analysis.
I annotated your tree below but could not name all clades because GenBank is presently out of order.
As your sample is identical with OP023244, I conclude that it is also identical with a Polish sample on Salix not in GenBank (BRA CR32406), which had both conidiomata and apothecia, the latter regrettably overmature. It is so far the only case with apothecia within this "laurisilvae-clade".
A. laurisilvae is only known from its anamorph. It looks very much like yours, despite the genetic distance (see below).
Do you have any idea about the substrate? It looks like a deciduous tree. Do you have the wood to check its anatomy?
I would be pleased to receive the collection data, also the sequence and its ab1 file. And it would be great if we could use your photos in our work. Do you have a scale for your pics?

Very strange is the amyloid reaction, we did not see that in any anamorph of Ascocoryne!

Zotto
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