Accès membres

Mot de passe perdu? S'inscrire

23-02-2026 11:22

Thomas Læssøe

https://svampe.databasen.org/observations/10584971

29-11-2024 21:47

Yanick BOULANGER

BonjourJ'avais un deuxième échantillon moins mat

07-02-2023 22:28

Ethan Crenson

Hello friends, On Sunday, in the southern part of

19-02-2026 17:49

Salvador Emilio Jose

Hola buenas tardes!! Necesito ayuda para la ident

09-02-2026 22:01

ruiz Jose

Hola, me paso esta colección en madera de pino, t

19-02-2026 13:50

Margot en Geert Vullings

We found this collection on deciduous wood on 7-2-

19-02-2026 12:01

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia (España), recole

17-02-2026 09:41

Maren Kamke Maren Kamke

Good morning, I found a Diaporthe species on Samb

16-02-2026 21:25

Andreas Millinger Andreas Millinger

Good evening,failed to find an idea for this fungu

08-12-2025 17:37

Lothar Krieglsteiner Lothar Krieglsteiner

20.6.25, on branch of Abies infected and thickened

« < 1 2 3 4 5 > »
Hysteropezizella diminuens
Nina Filippova, 12-02-2013 16:44
I saw very beautiful blue coloring of VBs at tips of paraphyses  in Zotto's collection of this species, 9413 (CRB), which i did not succeed to see. Is it vital reaction with cresil blue?

From dead leaves of Eriophorum angustifolium, wet bog hollow.

Apothecia immersed in tissue, ellipsoid, to 350 x 170 mk, opening by ellipsoid slit, disc pale, surrounded by dark brown edge.

Excipulum from textura angularis at base, cells dark brown, about 10 mk in diam, textura prismatica at the edge, end cells (hairs) cylindrical or clavate, 15-20 x 5-8, brown, walls of end cells under immersion with roughness; asci clavate, inamyloid, 59-67 x 12,5-14,6; paraphyses cylindrical with ampulliform end cell, about 3 mk broad and segmented in lower part, ampulliform cell up to 5 mk in broadest part, hyaline, with small guttules (rarely seen in rehydrated), not or some exeeding the asci; spores subfusoid, many-guttulate, 17 (15,7-17,7) x 3,7 (3,4-4,2) (Q=4,67; N=13).
  • message #21842
  • message #21842
  • message #21842
  • message #21842
  • message #21842
  • message #21842
Hans-Otto Baral, 12-02-2013 18:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, these are globose, rather inconspicuous (low-refractive) VBs which stain turquoise-blue in Cresylblue, but you see them only in living state.

Did you pretread your asci with KOH?

Hysteronaevia olivacea would have inamyloid asci, and paraph. as yours, but has much broader spores with acute ends.

Zotto

Nina Filippova, 12-02-2013 19:04
Re : Hysteropezizella diminuens
I used water-lugol, and MLZ and they were inamyloid, now added KOH, heated, and strong amyloid reaction happened. So, this shows they are actually amyloid?

Paraphyses hyaline and brownish in different fbs at one leaf, is seems variable.

I posted Hysteronaevia olivacea some before: http://www.ascofrance.fr/forum?page=1&id=21842
  • message #21851
Hans-Otto Baral, 12-02-2013 21:19
Hans-Otto Baral
Re : Hysteropezizella diminuens
This is the very typical hemiamyloid reaction, and it shows that your Lugol is insufficient. You should get a bright red reaction in water + lugol. Maybe your solution is almost colourless (the photo looks so), or it contains unallowed  ingredients. Hemiamyloid rings in Melzer are negative without KOH.

Sorry, I confused your two postings. So yes, this is H. diminuens. My answer on paraphyses, of course, concerned the Hysteronaevia, I will change it accordingly.

Zotto
Nina Filippova, 13-02-2013 11:55
Re : Hysteropezizella diminuens
There is ascus pore in fresh prepared lugol, it is still not pure red, apparently since J crystals not fully dissolved yet and solution is some weaker. But finally, i can see red reaction too.
  • message #21867
Hans-Otto Baral, 13-02-2013 12:09
Hans-Otto Baral
Re : Hysteropezizella diminuens
Yes, this is fine! Possibly the reaction has a blue component that becomes visible at lower iodine concentrations, resulting in a more dirty red (type RB).