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18-11-2018 18:41

Sven Heinz Sven Heinz

Hello together, does anyone have any idea what th

18-11-2018 10:49

PASCAL DUBOC

Bonjour j'ai aperçu ces minuscules asco en forme

14-11-2018 19:07

Andgelo Mombert

Bonsoir,Sur bois mort de feuillu. Apothécies sess

17-11-2018 08:51

Tanja Böhning Tanja Böhning

Bonjour,   J´ai un Asco sur mousse. Il mesure

17-11-2018 18:56

Pintos Angel Pintos Angel

Hello anybody has?Fasciatispora bicincta (Syd. &am

17-11-2018 13:23

Castillo Joseba Castillo Joseba

Me mandan el matgerial en seco desde GaliciaEn mad

17-11-2018 16:07

Lothar Krieglsteiner Lothar Krieglsteiner

... collected 29.10.2018 in the Biosphere Reservat

17-11-2018 12:09

Castillo Joseba Castillo Joseba

Me envian este material seco desde GaliciaCreciend

16-11-2018 19:57

Viktorie Halasu Viktorie Halasu

Hello forum,I'm looking for some protocol how to s

16-11-2018 20:32

Marc Detollenaere Marc Detollenaere

Hello forum,   Could someone help me with the f

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Heterosphaeria-like on Prunus padus
Viktorie Halasu, 16-04-2018 11:39
Viktorie HalasuHello forum,

I have an interesting discomycete found on bark of (living?) Prunus padus. First I thought Heterosphaeria cf patella, but:
- medulla is not gelified
- paraphyses apex looks different, not so enlarged, no VBs
- there's a gel layer on receptacle surface 
- different spore size, IKI+bb asci.
- excipulum turns dark brown-olive in KOH

Some characters remind me on Mollisia (KOH reaction, white hymenium when drying), but others on Heterosphaeria. Growing in lowland, former wetland forest (mostly Fraxinus and Populus spp.), Dermea padi and Biscogniauxia granmoi were found nearby. Apothecia 0,5-1 mm wide.
Thank you in advance for any advice.

Viktorie
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Hans-Otto Baral, 16-04-2018 12:11
Hans-Otto Baral
Re : Heterosphaeria-like on Prunus padus
Dear Viktorie

Great, I cannot remember such a fungus. Did you study the anamorph also? How sure does this belong to the apothecia in your opinion?

Zotto
Viktorie Halasu, 16-04-2018 12:30
Viktorie Halasu
Re : Heterosphaeria-like on Prunus padus
Dear Zotto,

thank you very much. I feared I'll get answer like this since I didn't find anything similar in your folder. : ) 

I didn't study it yet in detail, I'll try to do that today evening and test the KOH as well. But here's the upper part of one conidioma (pyknid? don't know correct term) where I measured the conidia and it has the same gel layer. I don't know how common this feature is among inoperculates. 

I wanted to test it with CRB, but still have some problems with this reagent - either it's too concentrated (and staind everything violet) or I get no reaction at all. Could you please advise me the correct way to apply it? I tried it with the medium becoming only light blue, which avoided the overstaining, but never got those beautiful turquise VBs (in other species).

Viktorie
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Hans-Otto Baral, 16-04-2018 13:12
Hans-Otto Baral
Re : Heterosphaeria-like on Prunus padus
I simply add CRB to the waer mount and let it diffuse. The size of the dopr determines the concentration. Of course, CRB must be aqueous, not dissolved in another reagent.

Not sure what you want, maybe staining of the gel layer? That should work, usually.