Hyaloscypha ? - Forum ASCOFrance

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Hyaloscypha ?

Ethan Crenson, 11-04-2019 06:24

Found last Sunday in New York City on unknown hardwood. I believe these tiny (.2 - .5mm) glassy cups fringed with delicate hairs are Hyaloscypha. Asci are weakly IKI+, 30 - 43 x 6 - 8µm. Spores are fusiform, hyaline, (6.5-) 7.5 - 9 x 2.5 - 3.5µm. Paraphyses are septate, about 2 - 2.5µm at the tips. Excipulum appeared to be textura angularis. I had a great deal of difficulty isolating the hairs and imaging them properly. Included here is a photo of what I suspect are the hairs of this collection, but I am not entirely sure.

In addition to the features I was expecting to see, I also saw a number of long needle-like spores (conidia perhaps?) measuring 18-30 x 1-1.5µm. Also, a single 1 septate spore slightly constricted at the center (contamination?).

I suspect that I have not provided enough information to ID this collection to species, but perhaps someone can help point me in the right direction.

Thank you!

Kosonen Timo, 11-04-2019 07:39

Re : Hyaloscypha ?

Hi,

Looks like Hyaloscypha all right. I think you have hairs at the margin of the apothecium in picture 11/12. The "hairs" in picture 12 are empty asci. The shape of the whole hair could help us here. Try cutting a tiny piece from the rim in order to get a good "hair to excipulum ratio", otherwise the hairs easily disappear under/on top of the hymenium/excipula. Squeezing might help but you easily just squash everything into a frustrating mess. Congo red might help also and give it some lugol / MLZ just to check the basic reactions.

so far you know:

-hardwood

-spore shape & size

-asci simple septate (no crozier)

-hairs pretty thin walled, not extremely long, smooth(?)

...if you follow the Hyaloscypha key or just glance through the common species and skip the ones with bent/glassy hairs you should be able to narrow it down to a small number of species.

Timo

Ethan Crenson, 11-04-2019 17:35

Re : Hyaloscypha ?

Timo,

Thank you for your very helpful comments. Is the key you recommend the synoptic key in "A monograph of Hyaloscypha and allied genera" by Seppo Huhtinen? I will make another attempt at isolating the hairs using your advice.

Ethan

Kosonen Timo, 12-04-2019 06:21

Re : Hyaloscypha ?

Yep, that's the one.

---taking an another look at the picture 12. It could be a hair in the end, not an asci. But it has had a rough life, so try to find nice & intact hairs somewhere. :-)

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