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21-10-2021 13:57

Miguel Ãngel Ribes Miguel Ángel Ribes

Buenos díasMe han pasado esta Scutellinia recogid

21-10-2021 15:44

François Bartholomeeusen

Dear forum members,On a rotten piece of wood I fou

21-10-2021 13:26

Chris Yeates Chris Yeates

Bonjour tous While processing images of this deli

21-10-2021 21:09

Enrique Rubio Enrique Rubio

I would be grateful for an opinion on this Orbilia

20-10-2021 08:29

Mirek Gryc

Hello all.I would ask for help in identifying this

21-10-2021 20:14

Ronald Morsink

These i found on the side of a dry creek in the Ne

20-10-2021 21:37

Garcia Susana

Hola, He recogido este ascomyceto que crece de m

10-10-2021 17:35

Marek Capoun Marek Capoun

And one more question from me for today.It was the

18-10-2021 23:10

Jorge Hernanz

Hemos encontrado éste Geoglossum en hábitat alpi

18-10-2021 18:59

Thorben Hülsewig

Hi there,3 days ago I found this ascomycet on leav

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black disco from the Bavarian forest
Lothar Krieglsteiner, 04-09-2021 21:31
Lothar Krieglsteiner... today on lying log of Fagus east of Spiegelau. The spores were about 8-11/5-5,5 µm (rough measurement), with IKI I could not obtain any reaction of the ascus or elsewhere. This was a surprise to me as I had thought of Rhizodiscina or Dactylospora.
What could it be else?
The spores were oil-rich, with hardly visible walls in H20 - it became more clear in IKI. The black globose excipulum and the filiform paraphyses do not give me a lot of nints.
Best, Lothar
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Hans-Otto Baral, 04-09-2021 21:51
Hans-Otto Baral
Re : black disco from the Bavarian forest
Hmm, did you test KOH whether some exudate dissolves? I assume there is no thallus at all?
Lothar Krieglsteiner, 04-09-2021 22:05
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
I just tried KOH - the picture became clear with better visible asci and excipulum (than in water and in IKI), but there was no )(or at least only few) pigment dissolving or anything like this.
The apothecia are nested in a black "slime" - or better "cream" but with no notable structure, so I do not think it is a (true) lichen, although I found algae cells in the "cream" I just examined. 
So - ???
Hans-Otto Baral, 04-09-2021 22:20
Hans-Otto Baral
Re : black disco from the Bavarian forest
Very difficult to say. It reminds me more of something like Agyrium than Helotiales. But Agyrium reacts with IKI (entire hymenium). A section of an apothecium would give additional insights. What is the apo diameter?
Lothar Krieglsteiner, 05-09-2021 07:20
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
thanks! I hope to find the time to try in the evening.
Yours, Lothar
Lothar Krieglsteiner, 05-09-2021 21:47
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
Hi Zotto,
here I made some fotos of a section - I hope it helps (?)
Again: no reaction with IKI.
Best, Lothar
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Hans-Otto Baral, 06-09-2021 11:50
Hans-Otto Baral
Re : black disco from the Bavarian forest
Good, but I fear I have no further idea. Do you think the dark brown spores are overmature ascospores of this fungus? They never occurred inside dead asci?
Lothar Krieglsteiner, 06-09-2021 21:24
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
Hello Zotto,
thanks anyway. I have no idea if the black spores are from the fungus or not.
So - not for the first time - a colletion remains unnamed.
Yours, Lothar
Zdenek Palice, 06-09-2021 21:38
Zdenek Palice
Re : black disco from the Bavarian forest
Hallo , what about Placynthiella lichen (Trapeliaceae), stunted/elderly specimen associated with a slime-cocktail of non-symbiotic algae, fungi and whatever? the asci are quite typical, the exciple should have a paraplectenchymatic structure with pigmented walls of hyphae but from some reason it is not clearly visible, the thallus which discriminates among single species is obscured unfortunately. I guess the brown spores were not released from asci. Zdenek
Lothar Krieglsteiner, 07-09-2021 09:14
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
Hello Zdenek,
thanks for your suggestion and information - I think you are right.
Best regards, Lothar
Hans-Otto Baral, 07-09-2021 09:17
Hans-Otto Baral
Re : black disco from the Bavarian forest
A good idea Zdenek! Morphologically I see much similarities to the photos and drawings in my folder in Baeomycetales (Ostropomycetidae). But the hemiamyloid hymenium would discriminate this genus. Lothar, did you test IKI after KOH-pretreatment? See image of Placynthiella icmalea and P. cf. uliginosa below.
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Lothar Krieglsteiner, 07-09-2021 10:21
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
Hello Zotto,
I tried IKI without KOH pretreatment. I will try later (I hope still today) with, and post again.
Thanks again, Lothar
Lothar Krieglsteiner, 07-09-2021 10:44
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
I just tried KOH and then IKI - no reaction. But possibly it is because the fungus is already quite old (?).
Hans-Otto Baral, 07-09-2021 11:16
Hans-Otto Baral
Re : black disco from the Bavarian forest
No, iodine reactions do not disappear. The material you showed looks quite fine.

I assume you washed the slide with water which must be done after KOH-pretreatment. You see it easily when afzet adding IKI or MLZ the iodine colour disappears because there is still too much KOH in the mount. I2 + KOH -> colourless KI.
Lothar Krieglsteiner, 07-09-2021 17:21
Lothar Krieglsteiner
Re : black disco from the Bavarian forest
hm - then it must be another fungus. I prepared another one (of the few still left) apothecia, put it in KOH 5per cent for some time, then moved it intensively through three successive drops of pure water, and then mounted in IKI. No reaction still. 
So it must remain unclear, I fear
Thanks again to you both, Zotto and Zdenek,
and best regards