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Ascobolus denudatus?
William Slosse, 28-01-2023 23:39
William SlosseGood evening everyone,

I recently bred an Ascobolus on droppings from Roe deer. I found the droppings in Rooiveld in Waardamme (West Flanders, Belgium).

The sessile, yellowish ascomata measure 1 to 2.5mm.
- Ascospores:
° ellipsoid with rounded/blunt tops.
° Smooth with mainly subparallel, longitudinal grooves
° Av(15) (17.22)17.95(18.93) x (9.46)9.74(10.73)µm
- Asus width: 22.66µm.
- Excipulum with subgolobose to pyriform cells with rusty brown walls (no evidence photo of).

Yi-Jian Yao's key brings me to denudatus.
Can anyone confirm/correct this determination?

William
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Michel Delpont, 29-01-2023 09:49
Michel Delpont
Re : Ascobolus denudatus?
Hello William.

If possible, could you show more photos of the spores and the tops of the paraphyses which must be encased in a yellowish-green pigment.

Michel.


William Slosse, 29-01-2023 10:13
William Slosse
Re : Ascobolus denudatus?
Good morning Michael,

I am currently trying to grow some more fruiting bodies.
I don't have many pictures of the spores and even less of the paraphyses.
These here are the rest.
I hope it is of some use to you.
Sincerely, William

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Michel Delpont, 29-01-2023 20:23
Michel Delpont
Re : Ascobolus denudatus?
Hello William.

I'm not sure your mushroom is denudatus; although there seem to be traces of a greenish mucus in places, the ornamentation of the spores does not resemble it. Maybe they are not ripe enough? Monitor your crops for a few days.

Michel.