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VASILEIOS KAOUNAS

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a spiraled paraphyses tiny Helotiale
Michel Hairaud, 04-06-2012 13:47
Michel HairaudHi to everyone, bonjour à tous
Quelqu'un pourrait il m'orienter pour cette récolte sur feuille de ronce ?
Would someone give me a clue for the following collection on dead Rubus leaves ?

Apos urcéolées, semi immergées dans l'epiderme de la feuille , diamètre 0,1-0.2 mm, gris foncé.
Asques H+, IKI bleu, 43_52 x 6,5-7
Spores 11-15 x 2-2,2 , clavées, G = 0,5-1 par de très fines guttules à ch. pôle
Paraphyses spiralées, dépassant largement les asques, remplies de vauoles réfringentes bleu-vert dans le CRB
EE text prismatica , prolongé par des poils de type ''Phialina''


Merci beaucoup par avance.
Amitiés
Michel

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Thomas Læssøe, 04-06-2012 14:29
Re : a spiraled paraphyses tiny Helotiale
JHP.11-134 from Sheffield/UK  down as Naeviopsis aff. orbilioides, det./del Zotto; photos JHP also on Rubus could be same :-) or the real N. orbilioides

cheers

Thomas
Petersen Jens H., 04-06-2012 15:00
Petersen Jens H.
Re : a spiraled paraphyses tiny Helotiale
On the UK finding (JHP-11.134; HB9515) :

Spores clavate to conical; spores smooth; spores without septae; spores in ligth microscope hyalin; spores not reacting to reagents; spores length 11 µm, width 2 µm; ascus wall unitunicat; ascus apical apparatus J+; ascus length 65 µm; with 8 spores; paraphyses as long as the asci; paraphyses cylindrical; paraphyses with coiled apices; paraphyses width 2 µm; textura prismatica; outer side smooth; excipulum hyalin. On/with members of Rosaceae; substrate/habitat leaves. [Description auto-generated from character input in MycoKey.]
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Hans-Otto Baral, 04-06-2012 15:55
Hans-Otto Baral
Re : a spiraled paraphyses tiny Helotiale
Cher Michel, hi guys

I also just looked at my drawing.... you have certainly the same, substrate is also identical.

There are a couple of described species with curled paraphyses, but they do not perfectly fit:

Pezizella orbiliodes Feltgen on Petasites, but the spores are smaller and the excipulummstains blue in iodine (not so in our fungus - you can test).

Naevia vitellina on Aegopodium is actually similar but the apothecia are elongate and break through the epidermis, though our fungus might also be erumpent.

Naevia lutescens was found in 2000 on Lamiastrum in Luxembourg, there the asci were simple-septate.

Calloria circinella on Cypripedium in China : also simple-septate and spores different.

Michel, did you actually see Phialina-hairs? Any photo? My section looks like this (live and in KOH)

Zotto
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Michel Hairaud, 04-06-2012 18:14
Michel Hairaud
Re : a spiraled paraphyses tiny Helotiale
Thanks, Thomas, Jens and Zotto ! 

I'm glad to hear from you.  Great document, this image of Zotto'hands drawing !

My fungus looks very similar to yours and I agree it doesn't quite match any of the known species cited though P. (or N.) orbilioides appears the closest among them  

Sorry Zotto the phialina type terminal cells of the EE concern another fungus .
Here are images of the ectal and the margin ''cells'' , up to 3,5 µm (only 2 µm fot the paraphyses) large and full with vbs. Only one free spore , which shows the same figure as yours .
My collection data : MH 50512 La Barre de Monts , Vendée, inner side of Rubus fructicosus leave , soc F. dumorum.

Amitiés
Michel
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